食品与发酵工业 >

2021 , Vol. 47 >Issue 3: 120 - 127

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024941

生产与科研应用

黑米中合成着色剂亮蓝的提取工艺优化及含量测定

  • 廖若宇 ,
  • 张春娥 ,
  • 刘新保 ,
  • 孙悦 ,
  • 田建文
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  • 1(宁夏回族自治区粮油产品质量检测中心,宁夏 银川,750001)
    2(宁夏农林科学院,宁夏 银川,750001)
硕士,工程师(张春娥高级工程师为通讯作者,E-mail:zhchune@163.com)

收稿日期: 2020-07-04

  修回日期: 2020-08-10

  网络出版日期: 2021-03-08

基金资助

宁夏回族自治区重点研发项目(2018BEG03070)

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Extraction process optimization and content determination of synthetic colorant bright blue in black rice

  • LIAO Ruoyu ,
  • ZHANG Chune ,
  • LIU Xinbao ,
  • SUN Yue ,
  • TIAN Jianwen
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  • 1(Ningxia Hui Autonomous Region Grain and Oil Product Quality Inspection Center, Yinchuan 750001, China)
    2(Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750001, China)

Received date: 2020-07-04

  Revised date: 2020-08-10

  Online published: 2021-03-08

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摘要

该文建立了一种黑米中亮蓝的提取与测定方法。采用NaHCO3为辅助提取试剂,样品经体积分数40%甲醇水萃取、离心,固相萃取小柱Strata-X-AW净化后采用高效液相色谱法定量分析。经验证,样品中加入0.52 g辅助提取剂NaHCO3,40%甲醇水提取液,涡旋12 min后离心,3次重复,能够将黑米中添加的亮蓝完全提取出来。合成着色剂亮蓝线性关系良好(R≥0.999 9),方法的检出限为0.02 mg/kg,定量限为0.05 mg/kg。加标回收率为81.70%～91.52%,相对标准偏差为0.39%～1.75%。该方法较GB 5009.35—2016操作方便快捷,简单准确,改进后的提取方法更适用于黑米及遇水易膨胀的谷物粉类制品中亮蓝的快速测定。

关键词： 黑米; 亮蓝; 碳酸氢钠; 液相色谱法; 固相萃取

本文引用格式

廖若宇 , 张春娥 , 刘新保 , 孙悦 , 田建文 . 黑米中合成着色剂亮蓝的提取工艺优化及含量测定[J]. 食品与发酵工业, 2021 , 47(3) : 120 -127 . DOI: 10.13995/j.cnki.11-1802/ts.024941

Abstract

This paper established a method for the extraction and determination of bright blue in black rice. Sodium bicarbonate was used as an auxiliary extraction reagent. The sample was firstly extracted with 40% methanol-water solution. Then after the centrifugation, it was further purified with a solid phase extraction column Strata-X-AW and followed by analysis using HPLC. It was verified that, with the addition of 0.52 g sodium bicarbonate and 40% methanol-water as extraction solvent, then vortex treatment for 12 minutes with three repetitions, the bright blue added in the black rice could be completely extracted. The results showed that the quantification method had a good linear relationship (R≥0.999 9), with the detection limit of 0.02 mg/kg and the qualification limit of 0.05 mg/kg. The recovery rate of standard addition was between 81.70% and 91.52%, and the relative standard deviation (RSD) was between 0.39 % and 1.75%. Compared with the method in GB 5009.35—2016, the current method is more convenient, quicker, simpler and more accurate. The improved extraction method is more suitable for the rapid determination of bright blue in black rice and cereal flour products, which are easy to swell in water.

Key words： black rice; bright blue; sodium bicarbonate; HPLC; solid phase extraction

参考文献

[1] 李静娜,黄常刚,梁高道,等.固相萃取-高效液相色谱法测定食品中的靛蓝和亮蓝[J].卫生研究,2017,46(2):318-323.
LI J N,HUANG C G,LIANG G D,et al.Determination of indigo and brilliant blue in different types of food products by high performance liquid chromatography with solid phase extraction[J].Journal of Hygiene Research,2017,46(2):318-323.
[2] WALESKA DE A S B,BRUNO P L,ANA P S P.Simultaneous determination of synthetic colorants in yogurt by HPLC[J].Food Chemistry,2016,197:1 347-1 347.
[3] MINIOTI K S,SAKELLARIOU C F,THOMAIDIS N S.Determination of 13 synthetic food colorants in water-soluble foods by reversed-phase high-performance liquid chromatography coupled with diode-array detector[J].Analytica Chimica Acta,2007,583(1):103-110.
[4] 邓六爱,张念英,郭冰,等.HPLC法测定茶叶中的6种合成着色剂[J].农产品加工,2019(14):49-51;54.
DENG L A,ZHANG N Y,GUO B,et al.Simultaneous analysis of synthetic colorants in tea by HPLC[J].Farm Products Processing,2019(14):49-51;54.
[5] MUHAMMAD I K M,MANAF O,SUJITH A,et al.Polymer thin films for chromatographic separation of plant pigments[J].Materials Letters,2019,252:321-324.
[6] 张倩茹,佟玲,田冬,等.薄层色谱法分离菠菜中天然色素的研究[J].蚌埠学院学报,2017,6(6):67-70.
ZHANG Q R,TONG L,TIAN D,et al.Study of preparation natural pigments in spinach with thin layer chromatography[J].Journal of Bengbu University,2017,6(6):67-70.
[7] 李丹梅. 高效液相法同时测定饮料中5种合成着色剂[J].食品安全导刊,2018(27):129.
LI D M.Simultaneous determination of 5 synthetic colorants in beverages by HPLC[J].China Food Safety Magazine,2018(27):129.
[8] 吕志勇,岳虹,陈静,等.高效液相色谱法测定发酵乳中着色剂的含量[J].乳业科学与技术,2018,41(5):20-23.
LV Z Y,YUE H,CHEN J,et al.Determination of food colorants in fermented milk by high performance liquid chromatography[J].Journal of Dairy Science and Technology,2018,41(5):20-23.
[9] MATHIYALAGAN S,MANDAL B K,LING Y C.Determination of synthetic and natural colorants in selected green colored foodstuffs through reverse phase-high performance liquid chromatography[J].Food Chemistry,2019,278:381-387.
[10] ARRIZABALAGA L A,RODRÍGUEZ P,MEDINA M,et al.Simultaneous analysis of natural pigments and E-141i in olive oils by liquid chromatography-tandem mass spectrometry[J].Analytical and Bioanalytical Chemistry,2019,411(21):5 577-5 591.
[11] 曾凯,罗明标,唐俊,等.改进QuEChERS-液质联用法测定食品中6种非食用色素[J].现代化工,2017,37(11):198-201.
ZENG K,LUO M B,TANG J,et al.Simultaneous determination of six inedible pigments in food by improved QuEChERS-UPLC-MS/MS method[J].Modern Chemical Industry,2017,37(11):198-201.
[12] ADIL E.Interference-free determination of carmine in food samples using ultrasonic assisted cloud point extraction coupled with spectrophotometry[J].Cumhuriyet Science Journal,2019,40(2):305-316.
[13] MEILING W,MINGZHU C,MIN Z,et al.Sensitive determination of Amaranth in foods using graphene nanomeshes[J].Journal of Electroanalytical Chemistry,2018,809:117-124.
[14] WENSHENG H,MIAO Z,WEIBING H.N-methyl-2-pyrrolidone-exfoliated graphene nanosheets as sensitive determination platform for amaranth at the nanomolar level[J].Ionics,2017,23(1):241-246.
[15] 王乐,郑新华,尚贞海,等.SERS法快速检测食品中新红[J].食品科技,2018,43(3):310-314.
WANG L,ZHENG X H,SHANG Z H,et al.Rapid screening newred in food by SERS[J].Food Science and Technology,2018,43(3):310-314.
[16] YUN F X,TING C,YA H G,et al.Rapid SERS detection of acid orange II and brilliant blue in food by using Fe3O4@Au core-shell substrate[J].Food Chemistry,2019(270):173-180.
[17] AI Y J,LIANG P,WU Y X,et al.Rapid qualitative and quantitative determination of food colorants by both Raman spectra and Surface-enhanced Raman Scattering (SERS)[J].Food chemistry,2018,241:427-433.
[18] KATERINA S M,CHRISTINA F S,NIKOLAOS S T.Determination of 13 synthetic food colorants in water-soluble foods by reversed-phase high-performance liquid chromatography coupled with diode-array detector[J].Analytica Chimica Acta,2006,583(1):103-110.
[19] 马先红,许海侠,韩昕纯.黑米的营养保健价值及研究进展[J].食品工业,2018,39(3):264-267.
MA X H,XU H X,HAN X C.Nutritional health value and research progress of black rice[J].The Food Industry,2018,39(3):264-267.
[20] 刘长姣,熊湘炜,郑霞,等.HPLC法测定黑米矢车菊素-3-葡萄糖苷[J].中国食品添加剂,2017(9):210-215.
LIU C J,XIONG X W,ZHENG X,et al.Detection of cyanidin-3-glucoside in black rice by HPLC[J].China Food Additives,2017(9):210-215.
[21] 刘长姣,郑霞,熊湘炜,等.分光光度法测定黑米花青素方法的建立[J].粮食与油脂,2019,32(1):73-77.
LIU C J,ZHENG X,XIONG X W,et al.Detection of anthocyanin in black rice by spectrophotometry[J].Cereals & Oils,2019,32(1):73-77.
[22] 晏晓清,张艳芳,陈芩,等.超声提取-液相色谱法检测黑米中花青素[J].食品安全导刊,2019(21):75-76.
YAN X Q,ZHANG Y F,CHEN Q,et al.Extraction of anthocyanins from rice by ultrasonic chromatography[J].China Food Safety Magazine,2019(21):75-76.
[23] 王治博,宋顺宗,张子波.黑米花色苷通过激活Nrf2/HO-1信号通路保护四氯化碳所致急性肝损伤的机制[J].时珍国医国药,2019,30(5):1 097-1 100.
WANG Z B,SONG S Z,ZHANG Z B.AEBR protects against carbon tetrachloride-induced liver injury via Nrf2/HO-1 activation[J].Lishizhen Medicine and Materia Medica Research,2019,30(5):1 097-1 100.
[24] 李祥,张晓寒,韩英,等.黑米花青素延缓果蝇衰老作用研究[J].食品科学技术学报,2020,38(1):74-79.
LI X,ZHANG X H,HAN Y,et al.Delaying aging effect of black rice anthocyanin on drosophila melanogaster[J].Journal of Food Science and Technology,2020,38(1):74-79.
[25] 厉成玲,周东浩.黑米多酚对四氧嘧啶诱导糖尿病小鼠的影响[J].食品安全质量检测学报,2020,11(10):3 189-3 193.
LI C L,ZHOU D H.Effect of black rice polyphenols on alloxan induced diabetes in mice[J].Journal of Food Safety & Quality,2020,11(10):3 189-3 193.
[26] 郭玲玲,杨悦,周寅生.马齿苋黑米蛋糕的研制[J].江苏调味副食品,2017(4):4-6;10.
GUO L L,YANG Y,ZHOU Y S.The study on processing technology of purslane and black rice cake[J].Jiangsu Condiment and Subsidiary Food,2017(4):4-6;10.
[27] 刘世娟,范业文,刘崇万,等.黑米红豆复合饮料的研制[J].食品研究与开发,2018,39(1):95-99.
LIU S J,FAN Y W,LIU C W,et al.Research on black rice and adzuki bean compound beverage[J].Food Research and Development,2018,39(1):95-99.
[28] 中华人民共和国国家卫生和计划生育委员会.GB 5009.35—2016,食品中合成着色剂的测定[S].北京:中国标准出版社,2016.
State Health and Family Planning Commission of the People's Republic of China.GB 5009.35—2016,Determination of synthetic colorants in food[S].Beijing:China Standard Press,2016.
[29] 周澍堃,曹雁平,黄正华.黑米色素在超声场中稳定性研究[J].中国食品添加剂,2015(3):71-76.
ZHOU S K,CAO Y P,HUANG Z H.Study on stability of the pigments from black rice under ultrasonic condition[J].China Food Additives,2015(3):71-76.
[30] 吴嘉彦,戴辉.食品中9种人工合成着色剂的检测[J].广东化工,2019,46(11):196-198.
WU J Y,DEI H.The Determination of nine synthetic colors in food[J].Guangdong Chemical Industry,2019,46(11):196-198.
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