建立了一种基于刷状纳米级ZnO富集,适配体特异性识别的电化学传感技术用于果品中展青霉素(patulin,PAT)的高灵敏度检测。ZnO纳米刷的引入可以有效增大PAT特异性适配体的负载量,进而增强对待测体系中目标物PAT的识别和捕获能力,实现PAT高灵敏度检测。结果表明,该方法检测PAT的线性范围是0.5~1×103 μg/L,最低检出限为0.15 μg/L,检测苹果汁和山楂片样品的平均加标回收率为94.75%～110.84%,对于赭曲霉毒素A具有较好的抗干扰能力。该方法结合可控形貌纳米材料富集了PAT适配体的生物识别能力,从而实现了对PAT的快速、准确、高灵敏度检测,为其他真菌毒素传感器的构建提供了一个新的思路。
In this paper, an electrochemical sensor technology based on brush-shaped nano-ZnO enrichment and aptamer specific recognition was established for high-sensitivity detection of patulin (PAT) in fruits. It was introduced to increase the load of PAT-specific aptamers, thereby enhancing the identification and capture capabilities of this sensing system for the target PAT, and realizing high-sensitivity detection of PAT. Results showed that the linear range of this method was 0.5-1×103 μg/L with the detection limit of 0.15 μg/L. And the average recovery of spiked apple juice and hawthorn tablets was between 94.75% and 110.84 % which has a good anti-interference ability for ochratoxin A. This method combined with the controllable morphology nanomaterials enriched the biorecognition ability of PAT aptamers, thereby could realize the rapid, accurate and high sensitivity detection of PAT, and provide a new idea for the construction of other mycotoxin sensors.
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