利用纳米金模拟酶在一定条件下能够催化过氧化氢氧化3,3',5,5'-四甲基联苯胺发生颜色反应,同时结合特异性高、亲和力强的核酸适配体建立了单增李斯特菌快速检测方法。通过对实验条件进行优化,建立了单增李斯特菌浓度与特定波长下吸光度值之间的关系式。线性回归方程为y=0.032 1x+0.024 8,R2=0.996 3,检出限约为5 CFU/mL,实际样品测定的回收率为93.5%～105.4%,相对标准偏差小于10%。该文方法特异性强、操作简单、成本低,能快速对食品中单增李斯特菌进行定量检测。
A method for rapid detection of Listeria monocytogenes was established using color reaction of H2O2-oxidized 3,3',5,5'-tetramethylbenzidine (TMB) catalyzed by gold nano-enzyme, combined with high specific and affiliative aptamer. Under optimized experimental conditions, the linear regression equation describing the relation between concentration of L. monocytogenes and absorbance at a specific wavelength was determined and expressed as y=0.032 1x+0.024 8 (R2=0.996 3). The detection limit was calculated as 5 CFU/mL, the sample recovery rate was 93.5%-105.4%, and the relative standard deviation was less than 10%. In summary, the method has the advantage of strong specificity, simple operation, and low cost, and can be used for rapid quantification of L. monocytogenes in foods.
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