综述与专题评论

新型纳米材料与噬菌体展示技术在真菌毒素检测中的应用

  • 冯林 ,
  • 陈雪岚
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  • 江西师范大学 生命科学学院,江西 南昌,330022
硕士研究生(陈雪岚教授为通讯作者,E-mail:xuelanchen162@163.com)

收稿日期: 2020-06-22

  修回日期: 2020-09-01

  网络出版日期: 2021-03-08

基金资助

国家自然科学基金项目(31960014);国家自然科学基金项目(31660019)

Application of new nanomaterials and phage display technology in mycotoxin detection

  • FENG Lin ,
  • CHEN Xuelan
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  • School of Life Science, Jiangxi Normal University, Nanchang 330022, China

Received date: 2020-06-22

  Revised date: 2020-09-01

  Online published: 2021-03-08

摘要

真菌毒素是真菌在生长繁殖过程中产生的次级有毒代谢产物,具有致癌、致畸、致突变的毒性作用,严重危害人体健康,因此对其检测与控制非常重要。现有检测方法普遍存在样品预处理复杂、成本高、操作繁琐等缺点,且会对操作人员和环境造成潜在二次危害。新型纳米材料可以提高真菌毒素的检测灵敏度,噬菌体展示技术可以筛选真菌毒素的模拟表位和抗真菌毒素的重组抗体,降低检测成本,达到无毒检测真菌毒素的目的。文章综述了近年来不同种类的纳米材料与噬菌体展示技术以及两者相互结合在真菌毒素检测方面的应用,并对其未来的发展进行了展望。

本文引用格式

冯林 , 陈雪岚 . 新型纳米材料与噬菌体展示技术在真菌毒素检测中的应用[J]. 食品与发酵工业, 2021 , 47(3) : 230 -236 . DOI: 10.13995/j.cnki.11-1802/ts.024842

Abstract

Mycotoxins are toxic secondary metabolites produced by some fungi during growth and reproduction. They have carcinogenic, teratogenic and mutagenic effects, which seriously endanger human health. Therefore, their detection and control are of great importance. However, the existing detection methods generally have the disadvantages of complex sample pretreatment, high cost and cumbersome operation, and may cause potential secondary hazards to operators and the environment. The detection sensitivity of mycotoxins can be improved with new nanomaterials, and the mimic epitopes of mycotoxins and the recombinant antibodies against mycotoxins can be screened through phage display technology, hence reducing the detection cost and achieving the purpose of non-toxic detection. The recent application of different nanomaterials and phage display technology and their combination in mycotoxin detection were reviewed, and the future development is prospected.

参考文献

[1] PINTO A P A V.Prevalence of mycotoxins in foods and decontamination[J].Current Opinion in Food Science,2017,14:50-60.
[2] AGRIOPOULOU S,STAMATELOPOULOU E,VARZAKAS T.Advances in occurrence,importance,and mycotoxin control strategies:Prevention and detoxification in foods[J].Foods (Basel,Switzerland),2020,9(2):137.
[3] PEREIRA V L F J O C.Mycotoxins in cereals and related foodstuffs:A review on occurrence and recent methods of analysis[J].Trends in Food Science&Technology,2014,36(2):96-136.
[4] ALSHANNAQ A,YU J.Occurrence,toxicity,and analysis of major mycotoxins in food[J].International Journal of Environmental Research and Public Health,2017,14(6):632.
[5] CHEN C,WU F.The need to revisit ochratoxin A risk in light of diabetes,obesity,and chronic kidney disease prevalence[J].Food and Chemical Toxicology,2017,103:79-85.
[6] MCMILLAN A,RENAUD J B,BURGESS K M N,et al.Aflatoxin exposure in Nigerian children with severe acute malnutrition[J].Food and Chemical Toxicology,2018,111:356-362.
[7] YANG Y,LI G,WU D,et al.Recent advances on toxicity and determination methods of mycotoxins in foodstuffs[J].Trends in Food Science & Technology,2020,96:233-252.
[8] KRSKA R,WELZIG E,BOUDRA H.Analysis of Fusarium toxins in feed[J].Animal Feed Science and Technology,2007,137(3-4):241-264.
[9] MARIN S,RAMOS A J,CANO-SANCHO G,et al.Mycotoxins:Occurrence,toxicology,and exposure assessment[J].Food and Chemical Toxicology,2013,60:218-237.
[10] RIZVI S A A,SALEH A M.Applications of nanoparticle systems in drug delivery technology[J].Saudi Pharmaceutical Journal,2018,26(1):64-70.
[11] KAITTANIS C,SANTRA S,PEREZ J M.Emerging nanotechnology-based strategies for the identification of microbial pathogenesis[J].Advanced Drug Delivery Reviews,2010,62(4):408-423.
[12] JANS H,HUO Q.ChemInform Abstract:Gold-nanoparticle-enabled biological and chemical detection and analysis[J].ChemInform,2012,41(7):2 849-2 866.
[13] JEEVANANDAM J,BARHOUM A,CHAN Y S,et al.Review on nanoparticles and nanostructured materials:History,sources,toxicity and regulations[J].Beilstein Journal of Nanotechnology,2018,9(1):1 050-1 074.
[14] EL-SAYED A,KAMEL M.Advances in nanomedical applications:Diagnostic,therapeutic,immunization,and vaccine production[J].Environmental Science and Pollution Research,2020,27(16):19 200-19 213.
[15] PIRO B,REISBERG S.Recent advances in electrochemical immunosensors[J].Sensors,2017,17(4):794.
[16] LAN L Y,YAO Y,PING J F,et al.Recent progress in nanomaterial-based optical aptamer assay for the detection of food chemical contaminants[J].ACS Applied Materials & Interfaces,2017,9(28):23 287-23 301.
[17] LAN L Y,YAO Y,PING J F,et al.Recent advances in nanomaterial-based biosensors for antibiotics detection[J].Biosens Bioelectron,2017,91:504-514.
[18] DAI S,WU S,DUAN N,et al.A luminescence resonance energy transfer based aptasensor for the mycotoxin ochratoxin A using upconversion nanoparticles and gold nanorods[J].Microchimica Acta,2016,183(6):1 909-1 916.
[19] MORENO V,MORENO V,ZOUGAGH M,et al.Hybrid nanoparticles based on magnetic multiwalled carbon nanotube-nano C18SiO2 composites for solid phase extraction of mycotoxins prior to their determination by LC-MS[J].Microchimica Acta,2016,183(2):871-880.
[20] 马小明, 孙密,林悦,等.基于金纳米材料的可视化生物传感器的研究进展[J].分析化学,2018,46(1):1-10.
MA X M,SUN M,LIN Y,et al.Progress of visual biosensor based on gold nanoparticles[J].Chinese Journal of Analytical Chemistry,2018,46(1):1-10.
[21] URUSOV A E,ZHERDEV A V,DZANTIEV B B.Use of gold nanoparticle-labeled secondary antibodies to improve the sensitivity of an immunochromatographic assay for aflatoxin B1[J].Microchimica Acta,2014,181(15-16):1 939-1 946.
[22] HE H,SUN D,PU H,et al.Bridging Fe3O4@Au nanoflowers and Au@Ag nanospheres with aptamer for ultrasensitive SERS detection of aflatoxin B1[J].Food Chemistry,2020,324:126 832.
[23] ZHANG J,XU X,QIANG Y.Ultrasensitive electrochemical aptasensor for ochratoxin A detection using AgPt bimetallic nanoparticles decorated iron-porphyrinic metal-organic framework for signal amplification[J].Sensors and Actuators B:Chemical,2020,312:127 964.
[24] HUANG X,AGUILAR Z P,XU H,et al.Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection:A review[J].Biosensors and Bioelectronics,2016,75:166-180.
[25] YANG C,DENNO M E,PYAKUREL P,et al.Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules:A review[J].Analytica Chimica Acta,2015,887:17-37.
[26] LIU N,NIE D,TAN Y,et al.An ultrasensitive amperometric immunosensor for zearalenones based on oriented antibody immobilization on a glassy carbon electrode modified with MWCNTs and AuPt nanoparticles[J].Microchimica Acta,2017,184(1):147-153.
[27] SHAO M,YAO M,SAEGER S D,et al.Carbon quantum dots encapsulated molecularly imprinted fluorescence quenching particles for sensitive detection of zearalenone in corn sample[J].Toxins,2018,10(11):438.
[28] LI Z,XUE N,MA H,et al.An ultrasensitive and switch-on platform for aflatoxin B1 detection in peanut based on the fluorescence quenching of graphene oxide-gold nanocomposites[J].Talanta,2018,181:346-351.
[29] CAO M,LI Z,WANG J,et al.Food related applications of magnetic iron oxide nanoparticles:Enzyme immobilization,protein purification,and food analysis[J].Trends in Food Science & Technology,2012,27(1):47-56.
[30] SHARIFI S V S Z A.Detection of pathogenic bacteria via nanomaterials-modified aptasensors[J].Biosensors & Bioelectronics,2019,150:111 933.
[31] WANG C Q J W K.Colorimetric aptasensing of ochratoxin A using Au@Fe3O4 nanoparticles as signal indicator and magnetic separator[J].Biosens.Bioelectro,2016,77:1 183-1 191.
[32] URUSOV A E,PETRAKOVA A V,VOZNIAK M V,et al.Rapid immunoenzyme assay of aflatoxin B1 using magnetic nanoparticles[J].Sensors (Basel,Switzerland),2014,14(11):21 843-21 857.
[33] HAO N,JIANG L,QIAN J,et al.Ultrasensitive electrochemical ochratoxin a aptasensor based on cdte quantum dots functionalized graphene/Au nanocomposites and magnetic separation[J].Journal of Electroanalytical Chemistry,2016,781:332-338.
[34] HENDRICKSON O D,CHERTOVICH J O,ZHERDEV A V,et al.Ultrasensitive magnetic ELISA of zearalenone with pre-concentration and chemiluminescent detection[J].Food Control,2018,84:330-338.
[35] BONILLA J C,BOZKURT F,ANSARI S,et al.Applications of quantum dots in food science and biology[J].Trends in Food Science & Technology,2016,53:75-89.
[36] ZHANG L,YING Y B,LI Y B,et al.Integration and synergy in protein-nanomaterial hybrids for biosensing:Strategies and in-field detection applications[J].Biosensors and Bioelectronics,2020.DOI:10.1016/j.bios.2020.112036.
[37] EL-SAYED A,KAMEL M.Advanced applications of nanotechnology in veterinary medicine[J].Environmental Science and Pollution Research International,2018,27(16):19 073-19 086.
[38] DUAN H,LI Y,SHAO Y,et al.Multicolor quantum dot nanobeads for simultaneous multiplex immunochromatographic detection of mycotoxins in maize[J].Sensors and Actuators B:Chemical,2019,291:411-417.
[39] GUO P,YANG W,HU H,et al.Rapid detection of aflatoxin B1 by dummy template molecularly imprinted polymer capped CdTe quantum dots[J].Analytical and Bioanalytical Chemistry,2019,411(12):2 607-2 617.
[40] ZHANG X,YU X,WANG J,et al.One-step core/multishell quantum dots-based fluoroimmunoassay for screening of deoxynivalenol in maize[J].Food Analytical Methods,2018,11(9):2 569-2 578.
[41] ZHANG J,CHENG F,LI J,et al.Fluorescent nanoprobes for sensing and imaging of metal ions:Recent advances and future perspectives[J].Nano Today,2016,11(3):309-329.
[42] WU S,DUAN N,ZHU C,et al.Magnetic nanobead-based immunoassay for the simultaneous detection of aflatoxin B1 and ochratoxin A using upconversion nanoparticles as multicolor labels[J].Biosensors and Bioelectronics,2011,30(1):35-42.
[43] SUN C,LI H,KOIDIS A,et al.Quantifying aflatoxin B1 in peanut oil using fabricating fluorescence probes based on upconversion nanoparticles[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2016,165:120-126.
[44] 张莹莹, 钱志娟,谢正军,等.基于上转换荧光纳米粒子和金纳米粒子间荧光共振能量转移的高灵敏赭曲霉毒素A检测方法研究[J].分析测试学报,2018,37(1):31-38.
ZHANG Y Y,QIAN Z J,XIE Z J,et al.Highly sensitive detection of ochratoxin a based on FRET from upconversion nanoparticles to gold nanoparticles[J].Journal of Instrumental Analysis,2018,37(1):31-38.
[45] BAGHERI N,KHATAEE A,HABIBI B,et al.Mimetic Ag nanoparticle/Zn-based MOF nanocomposite (AgNPs@ZnMOF) capped with molecularly imprinted polymer for the selective detection of patulin[J].Talanta,2018,179:710-718.
[46] GU Y,WANG Y,WU X,et al.Quartz crystal microbalance sensor based on covalent organic framework composite and molecularly imprinted polymer of poly(o-aminothiophenol) with gold nanoparticles for the determination of aflatoxin B1[J].Sensors and Actuators B:Chemical,2019,291:293-297.
[47] LI M,QIAO S,ZHENG Y,et al.Fabricating covalent organic framework capsules with commodious microenvironment for enzymes[J].Journal of the American Chemical Society,2020,142(14):6 675-6 681.
[48] 邬亭亭, 刘平,罗永艾.噬菌体在癌症治疗研究中的应用[J].世界科技研究与发展,2012,34(3):482-484;496.
WU T T,LIU P,LUO Y A.Applications of phases in cancer treatment studies[J].World Sci-Tech R & D,2012,34(3):482-484;496.
[49] ZANGANEH S,ROUHANI NEJAD H,MEHRABADI J F,et al.Rapid and sensitive detection of staphylococcal enterotoxin B by recombinant nanobody using phage display technology[J].Applied Biochemistry and Biotechnology,2019,187(2):493-505.
[50] 何庆华, 刘仁荣,许杨.利用噬菌体肽库淘选玉米赤霉烯酮的模拟表位[J].食品科学,2007,28(8):241-243.
HE Q H,LIU R R,XU Y.Biopanning of zearalenone mimotope by phage displayed peptide bank[J].Food Science,2007,28(8):241-243.
[51] HE Z,HE Q,XU Y,et al.Ochratoxin A mimotope from second-generation peptide library and its application in immunoassay[J].Analytical Chemistry,2013,85(21):10 304-10 311.
[52] ZOU X Q,CHEN C C,HUANG X L,et al.Phage-free peptide ELISA for ochratoxin A detection based on biotinylated mimotope as a competing antigen[J].Talanta,2016,146:394-400.
[53] WANG X,HE Q,XU Y.Anti-idiotypic VHH phage display-mediated immuno-PCR for ultrasensitive determination of mycotoxin zearalenone in cereals.[J].Talanta,2016:410-415.
[54] HE T W Y L.Nanobody-based enzyme immunoassay for aflatoxin in agro-products with high tolerance to cosolvent methanol[J].Analytucal Chemistry,2014,86(17):8 873-8 880.
[55] SOMPUNGA P,PRUKSAMETANAN N,RANGNOI K,et al.Generation of human and rabbit recombinant antibodies for the detection of Zearalenone by phage display antibody technology[J].Talanta,2019,201:397-405.
[56] NGO-DUC T,PLANK J M,CHEN G,et al.M13 bacteriophage spheroids as scaffolds for directed synthesis of spiky gold nanostructures[J].Nanoscale,2018,10(27):13 055-13 063.
[57] YI H,GHOSH D,HAM M,et al.M13 Phage-functionalized single-walled carbon nanotubes as nanoprobes for second near-infrared window fluorescence imaging of targeted tumors[J].Nano Letters,2012,12(3):1 176-1 183.
[58] NGUYEN A H,SHIN Y,SIM S J.Development of SERS substrate using phage-based magnetic template for triplex assay in sepsis diagnosis[J].Biosensors and Bioelectronics,2016,85:522-528.
[59] HUANG S,QI J,DEQUILETTES D W,et al.M13 Virus-based framework for high fluorescence enhancement[J].Small,2019,15(28):1 901 233.
[60] GUO Y,LIANG X,ZHOU Y,et al.Construction of bifunctional phage display for biological analysis and immunoassay[J].Analytical Biochemistry,2010,396(1):155-157.
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