食品与发酵工业 >

2021 , Vol. 47 >Issue 4: 15 - 20

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025071

研究报告

好食脉孢霉发酵产类胡萝卜素的鉴定、抗氧化性及稳定性研究

  • 邓永平 ,
  • 车鑫 ,
  • 艾瑞波 ,
  • 刘晓兰 ,
  • 辛嘉英 ,
  • 王晓杰
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  • 1(齐齐哈尔大学 食品与生物工程学院,黑龙江 齐齐哈尔,161006)
    2(黑龙江省玉米深加工理论与技术重点实验室,黑龙江 齐齐哈尔,161006)
    3(哈尔滨商业大学,省高校食品科学与工程重点实验室,黑龙江 哈尔滨,150076)
博士,教授(辛嘉英教授为通讯作者,E-mail:xinjiayingvip@163.com)

收稿日期: 2020-07-15

  修回日期: 2020-09-10

  网络出版日期: 2021-03-16

基金资助

黑龙江省省属高等学校基本科研业务费科研项目“粮头食尾”专项(LTSW201740);黑龙江省省属高等学校基本科研业务费科研项目校地合作项目(135409507);黑龙江省自然科学基金项目(LH2020C063)

收起

Identification, antioxidant and stability of carotenoids from a GRAS fungus Neurospora sitophila

  • DENG Yongping ,
  • CHE Xin ,
  • AI Ruibo ,
  • LIU Xiaolan ,
  • XIN Jiaying ,
  • WANG Xiaojie
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  • 1(College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China)
    2(Key Laboratory of Corn Deep Proessing Theory and Techology, Qiqihar University, Qiqihar 161006, China)
    3(Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China)

Received date: 2020-07-15

  Revised date: 2020-09-10

  Online published: 2021-03-16

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摘要

利用液相色谱-质谱联用技术对好食脉孢霉(Neurospora sitophila)固态发酵生产的类胡萝卜素进行初步鉴定,研究了类胡萝卜素粗提物的抗氧化活性和稳定性。N.sitophila来源的类胡萝卜素的主要成分之一为β-胡萝卜素;当质量浓度为6 μg/mL时,类胡萝卜素的DPPH自由基清除率显著高于α-生育酚、丁基羟基茴香醚(butyl hydroxylanisole, BHA)和β-胡萝卜素(P＜0.05),羟自由基清除率显著高于α-生育酚、BHA、特丁基对苯二酚(tert-butyl hydroquinone, TBHQ)和β-胡萝卜素(P＜0.05),总还原力显著高于α-生育酚和β-胡萝卜素(P＜0.05);类胡萝卜素在避光、低温及不含K+、Na+、Mg2+、Ca2+、Ba2+、Cu2+、Fe3+的条件下较稳定。研究结果表明好食脉孢霉类胡萝卜素具有较强的抗氧化活性,具有开发为天然抗氧化剂的巨大潜力。

关键词： 好食脉孢霉; 类胡萝卜素; 液相色谱-质谱技术; 抗氧化活性

本文引用格式

邓永平 , 车鑫 , 艾瑞波 , 刘晓兰 , 辛嘉英 , 王晓杰 . 好食脉孢霉发酵产类胡萝卜素的鉴定、抗氧化性及稳定性研究[J]. 食品与发酵工业, 2021 , 47(4) : 15 -20 . DOI: 10.13995/j.cnki.11-1802/ts.025071

Abstract

A liquid chromatography-mass spectrometry was developed for preliminary identification of carotenoids produced by Neurospora sitophila through solid-state fermentation, then antioxidant activity and stability of carotenoids were investigated. One of the main components of carotenoids from N. sitophila was β-carotene. When the carotenoid concentration was 6 μg/mL, the DPPH· clearance rate of carotenoids was significantly higher than that of α-tocopherol, butyl hydroxylanisole(BHA) and β-carotene (P<0.05);the ·OH clearance rate was significantly higher than that of α-tocopherol, BHA, tert-butyl hydroquinone(TBHQ) and β-carotene (P<0.05); the total reduction capacity of carotenoids was significantly higher than that of α-tocopherol and β-carotene (P<0.05). Carotenoids were relatively stable under the conditions of avoiding light, low temperature and free of some ions such as K+, Na+, Mg2+, Ca2+, Ba2+, Cu2+ and Fe3+. The above results showed that carotenoids from N. sitophila have excellent antioxidant activities, indicating great potential as natural antioxidants.

Key words： Neurospora Sitophila; carotenoids; liquid chromatography-mass spectrometry technology; antioxidant activity

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