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植物来源的黄酮类化合物抗体的制备和应用研究进展

  • 赵静 ,
  • 秦艳 ,
  • 左巍 ,
  • 崔永亮 ,
  • 焦必宁
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  • 1(西南大学,柑桔研究所,重庆,400712)
    2(农业部柑桔及苗木质量监督检测测试中心,重庆,400712)
硕士研究生(焦必宁研究员为通讯作者,E-mail:jiaobining@cric.cn)

收稿日期: 2020-10-09

  修回日期: 2020-11-10

  网络出版日期: 2021-03-16

基金资助

国家现代农业(柑桔)产业技术体系建设专项(CARS-26);国家农产品质量安全风险评估重大专项(GJFP2019043);重庆市自然科学基金(cstc2018jcyjAX0662)

Research progress on preparation and application of flavonoids antibody of plants

  • ZHAO Jing ,
  • QIN Yan ,
  • ZUO Wei ,
  • CUI Yongliang ,
  • JIAO Bining
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  • 1(Citrus Research Institute,Southwest University,Chongqing 400712,China)
    2(Quality Supervision and Testing Center for Citrus and Seedling,Ministry of Agriculture,Chongqing 400712,China)

Received date: 2020-10-09

  Revised date: 2020-11-10

  Online published: 2021-03-16

摘要

黄酮类化合物是一类重要的天然产物。流行病学研究表明,黄酮类化合物可以降低罹患多种慢性病及癌症的风险,且对新冠肺炎COVID-19也有显著的辅助疗效。抗体是现代生命科学研究中极其重要的工具,黄酮类化合物的抗体在中医药及食品营养科学研究领域具有广阔的应用前景。该文简述了黄酮类化合物的药理作用,总结了国内外黄酮类化合物半抗原、完全抗原和抗体的制备进展,并对黄酮类化合物抗体的应用(主要包括酶联免疫分析、免疫亲和色谱方法、荧光免疫分析方法、免疫层析试纸条和Eastern blotting法)进行了阐述,为黄酮类化合物抗体的进一步研究提供参考。

本文引用格式

赵静 , 秦艳 , 左巍 , 崔永亮 , 焦必宁 . 植物来源的黄酮类化合物抗体的制备和应用研究进展[J]. 食品与发酵工业, 2021 , 47(4) : 284 -292 . DOI: 10.13995/j.cnki.11-1802/ts.025784

Abstract

Flavonoids are important natural products. Epidemiological studies have shown that flavonoids can reduce the risk of a variety of chronic diseases and cancer, they also have a significant auxiliary effect on COVID-19. Antibodies are extremely important tools in modern life science research. Flavonoid antibody technology has broad application prospects in the fields of Chinese medicine and food nutrition science research. This article introduced the pharmacological effects of flavonoids and the research progress of the preparation of flavonoid haptens, complete antigen and antibodies. Besides, the application of flavonoid antibody which mainly includes enzyme-linked immunoassay, immunoaffinity chromatography method, fluorescence immunoassay method, immunochromatographic test strips and eastern blotting were illustrated, thus supporting the reference for further research of flavonoid antibodies.

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