分离、纯化获得1株高产β-葡聚糖的黄伞菌株PA-008,为进一步提高该菌株中β-葡聚糖的产量和产率,通过单因素和正交试验,对培养基成分及液态发酵条件进行优化。优化得到的发酵条件为:碳源为葡萄糖,氮源为酵母浸出粉,发酵温度25 ℃,初始pH值6.0。同时,采用体外消化模型对黄伞菌丝体中主要营养物质(氨基酸、矿物质、β-葡聚糖)的生物可利用率进行分析。结果表明,黄伞菌丝体中的氨基酸及矿物质均有一定程度的释放,且重金属矿物质(Pb、Cd、As)经体外模拟消化后均低于安全值,消化液中β-葡聚糖含量为41.92 mg/g,生物可利用率达15.50%,实验所获数据为更科学和深入地评估黄伞菌株提供了理论基础。
韩宛芸
,
张长懿
,
顾泽鹏
,
段小雨
,
孙庆杰
,
邱立忠
,
卞希良
,
邬应龙
,
刘韫滔
. 高产β-葡聚糖的黄伞菌株分离、鉴定及其体外模拟消化[J]. 食品与发酵工业, 2021
, 47(7)
: 51
-57
.
DOI: 10.13995/j.cnki.11-1802/ts.025773
A Pholiota adiposa strain PA-008 with a high β-glucan-producing property (GenBank accession number:MF687867) was obtained by isolation and purification.In order to further improve the production and yield of β-glucan of strain, single-factor and orthogonal experiments were carried out to design and optimize the medium composition and liquid fermentation conditions of PA-008.The results showed that the optimal fermentation conditions of the strains of PA-008 were as follows:glucose, yeast powder, fermentation temperature 25 ℃, initial pH value 6.0.Meanwhile, an in vitro digestion model was used to analyze the bioavailability of the main nutrients (amino acids, minerals, and β-glucans) in the P.adiposa.The results showed that there was a certain degree of amino acids and minerals release in the mycelium of the P.adiposa.And the release of heavy metal minerals (Pb, Cd, As) were lower than the safe value after in vitro simulated digestion.The β-glucose content in the digestive juice was 41.92 mg/g, and its bioavailability reached 15.50%.The above data provided a theoretical basis for a more scientific and in-depth evaluation of the P.adiposa.
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