研究报告

海参酶解物促SD大鼠皮肤创面愈合效果的研究

  • 程敏君 ,
  • 胡锦华 ,
  • 唐雪 ,
  • 杨亭亭 ,
  • 周鹏 ,
  • 胡炜
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  • 1(食品科学与技术国家重点实验室(江南大学),江苏 无锡,214122)
    2(江南大学 食品学院,江苏 无锡,214122)
    3(山东好当家海洋发展股份有限公司,山东 威海,264200)
硕士研究生(周鹏教授为通讯作者,E-mail:zhoupeng@jiangnan.edu.cn)

收稿日期: 2020-10-18

  修回日期: 2020-11-08

  网络出版日期: 2021-05-20

基金资助

食品科学与技术国家一级学科计划项目(JUFSTR20180201)

Effect of sea cucumber enzymatic hydrolysate on skin wound healing in SD rats

  • CHENG Minjun ,
  • HU Jinhua ,
  • TANG Xue ,
  • YANG Tingting ,
  • ZHOU Peng ,
  • HU Wei
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  • 1(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
    2(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
    3(Shandong Homey Aquatic Development Co.Ltd.,Weihai 264200,China)

Received date: 2020-10-18

  Revised date: 2020-11-08

  Online published: 2021-05-20

摘要

目的:为探究海参酶解物(sea cucumber enzymatic hydrolysate,SCH)对SD大鼠皮肤创面愈合的影响,为SCH应用于创面愈合提供理论依据。方法:设立模型组、3个SCH剂量组和阳性组,建立大鼠全层皮肤切除模型,于术后第4、8和12天分批处死。通过计算创面愈合率、进行组织切片伊红染色(hematoxylin-eosin,H&E)和马松三色染色(Masson’s trichrome method,Masson),以及结合血液中白细胞介素-8(interleukin-8,IL-8)和白细胞介素-10(interleukin-10、IL-10)、创面处羟脯氨酸(hydroxyproline,Hyp)和血管内皮生长因子(vascular endothelial growth factor,VEGF)、表皮生长因子(epidermal growth factor,EGF)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的含量,综合分析SCH对创面愈合的影响。结果表明:SCH各剂量组的创面愈合率均高于模型组;H&E结果显示SCH可以调节炎性细胞浸润、促进血管新生,且中剂量组表皮恢复最快;IL-8和IL-10结果显示SCH可通过调节其分泌量来缩短炎症反应时间;Masson染色观察到SCH显著促进创面处胶原纤维的生成,且Hyp含量相应增加。此外,SCH促创面愈合机制可能与生长因子分泌量显著增加有关。结论:SCH具有促SD大鼠创面愈合的功效,中剂量组(0.50 g/kg)的促愈合效果最佳。

本文引用格式

程敏君 , 胡锦华 , 唐雪 , 杨亭亭 , 周鹏 , 胡炜 . 海参酶解物促SD大鼠皮肤创面愈合效果的研究[J]. 食品与发酵工业, 2021 , 47(7) : 94 -101 . DOI: 10.13995/j.cnki.11-1802/ts.025916

Abstract

The impact of sea cucumber hydrolysate (SCH) on skin wound healing in SD rats was investigated, aiming to provide basis for the application of SCH in wound healing.A rat model of full-thickness cutaneous wounds was established in a model group, three SCH dose groups and a positive group, and the rats were sacrificed in batches on the 4th, 8th and 12nd day after surgery.The effect of SCH on wound healing was comprehensively analyzed in combination with the results of wound healing rate, observation of tissues stained with hematoxylin-eosin (H&E) and Masson’s trichrome method (Masson), changes of interleukin-8 (IL-8) and interleukin-10 (IL-10) in the blood, detection of hydroxyproline (Hyp), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) in wounds.Results showed that the wound healing rate of each dose group of SCH was faster than that of the model group.H&E results showed that SCH could regulate the infiltration of inflammatory cells and promote angiogenesis, and the epidermis recovered fastest in the medium dose group.Based on the results of IL-8 and IL-10, SCH could regulate their secretion to reduce the inflammatory reaction duration.Collagen fibers formation in the wound significantly promoted by SCH was observed in Masson’s trichrome stained tissues, and Hyp content increased accordingly.In addition, the mechanism of SCH wound healing may be related to the significant increase of growth factors secretion.The prepared SCH could effectively promote wound healing in SD rats, with best performance observed in the medium dose group (0.50 g/kg).

参考文献

[1] 穆琳.加工对海参营养和功能成分的研究[D].大连:大连海洋大学,2016.
MU L.Studies of processing method on nutritional and functional compositions of instant sea cucumber products[D].Dalian:Dalian Ocean University,2016.
[2] 耿瑞,岳冬冬,刘龙腾,等.产业上升期企业经营活动对海参市场扩大的影响—基于大连加工企业的调查[J].安徽农业科学,2017,45(30):229-233.
GENG R,YUE D D,LIU L T,et al.Impact of business activities on the expansion of sea cucumber industry-An investigation on the processing enterprises in Dalian[J].Journal of Anhui Agricultural Sciences,2017,45(30):229-233.
[3] 何舟.不同饲料对刺参生长、体成分、免疫相关酶和消化酶活性的影响[D].大连:大连海洋大学,2014.
HE Z.Studies on the effects of different diets on the growth,body composition,non-specific immunity and digestive enzymes activities of sea cucumber[D].Dalian:Dalian Ocean University,2014.
[4] KIANI N,HEIDARI B,RASSA M,et al.Antibacterial activity of the body wall extracts of sea cucumber (Invertebrata;Echinodermata) on infectious oral streptococci[J].Journal of Basic and Clinical Physiology and Pharmacology,2014,25(4):367-373.
[5] GUAN R,PENG Y,ZHOU L,et al.Precise structure and anticoagulant activity of fucosylated glycosaminoglycan from Apostichopus japonicus:Analysis of its depolymerized fragments[J].Marine Drugs,2019,17(4):195.
[6] 张晓羽,李楠,董秀芳,等.海参水煮液多糖提取物体外抗炎活性的研究[J].食品与药品,2018,20(2):81-85.
ZHANG X Y,LI N,DONG X F,et al.Study on anti-inflammatory activity of polysaccharide extracts from sea cucumber processing liquor[J].Food and Drug,2018,20(2):81-85.
[7] 何丽霞,陈启贺,刘睿,等.海参寡肽海参寡肽:免疫调节作用及机制研究[J].科技导报,2016,34(11):42-47.
HE L X,CHEN Q H,LIU R,et al.Sea cucumber oligopeptides:Immunomodulatory effects and its mechanism[J].Science & Technology Review,2016,34(11):42-47.
[8] BROWN K L,PHILLIPS T J.Nutrition and wound healing[J].Clinics in Dermatology,2010,28(4):432-439.
[9] HE M,SUN L,FU X,et al.Biodegradable amino acid-based poly (ester amine) with tunable immunomodulating properties and their in vitro and in vivo wound healing studies in diabetic rats’wounds[J].Acta Biomaterialia,2019,84:114-132.
[10] BONVINI A,COQUEIRO A Y,TIRAPEGUI J,et al.Immunomodulatory role of branched-chain amino acids[J].Nutrition Reviews,2018,76(11):840-856.
[11] 宋思媛,李美琪,王泽旭,等.海参多糖对小鼠肠道菌群紊乱的恢复作用[J].中国微生态学杂志,2019,31(9):1 023-1 026.
SONG S Y,LI M Q,WANG Z X,et al.Restoration effect of sea cucumber polysaccharides on intestinal microbiota disorder in mice[J].Chinese Journal of Microecology,2019,31(9):1 023-1 026.
[12] 高芮.海参加工液中蛋白及多糖的提取工艺[D].大连:大连理工大学,2019.
GAO R.Extraction process of polysaccharide and protein from waste liquor of processing[D].Dalian:Dalian University of Technology,2019.
[13] 付学军,金海珠.酶解时间对海参肽抗氧化活性影响的研究[J].食品科技,2013,38(9):193-197.
FU X J,JIN H Z.Effect of enzymatic hydrolysis time on antioxidant activity of sea cucumber peptide[J].Food Science and Technology,2013,38(9):193-197.
[14] 郑志鸿,章超桦,林海生,等.方格星虫酶解物对小鼠皮肤创伤修复的作用[J].广东海洋大学学报,2020,40(1):97-103.
ZHENG Z H,ZHANG C H,LIN H S,et al.Promoting the skin wound healing in mice using Sipunculus nudus hydrolysate[J].Journal of Guangdong Ocean University,2020,40(1):97-103.
[15] 李林,李迪,徐腾,等.海参胶原低聚肽对糖尿病小鼠术后伤口愈合的促进作用[J].中国食物与营养,2017,23(7):71-75.
LI L,LI D,XU T,et al.Effect of sea cucumber collagen oligopeptide in diabetic wound heating[J].Food and Nutrition in China,2017,23(7):71-75.
[16] 黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004,9(9):1 069-1 072.
HUANG J H,HUANG X H,CHEN Z Y,et al.Dose conversion among different animals and healthy volunteers in pharmacological study[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2004,9(9):1 069-1 072.
[17] YANG T,ZHANG K,LI B,et al.Effects of oral administration of peptides with low molecular weight from Alaska Pollock (Theragra chalcogramma) on cutaneous wound healing[J].Journal of Functional Foods,2018,48:682-691.
[18] YAN J,LIAO X,LI S,et al.Effects of carbon arc lamp irradiation on wound healing in a rat cutaneous full-thickness wound model[J].Photobiomodulation Photomedicine and Laser Surgery,2019,37(1):17-24.
[19] CHE HAMZAH N,MOHAMMED A,SIRAJUDEEN K,et al.Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats[J].Asian Pacific Journal of Tropical Biomedicine,2019,9(4):140-149.
[20] 高菲.刺参(Apostichopus japonicus)营养成分,食物来源及消化生理的季节变化[D].山东:中国科学院海洋研究所,2008.
GAO F.Seasonal variations of nutritional composition,food resources,and digestive physiology in sea cucumber (Apostichopus japonicus)[D].Shandong:Institute of Oceanology,Chinese Academy of Sciences,2008.
[21] BHUIMBAR M V,BHAGWAT P K,DANDGE P B.Extraction and characterization of acid soluble collagen from fish waste:Development of collagen-chitosan blend as food packaging film[J].Journal of Environmental Chemical Engineering,2019,7(2):102 983
[22] XU Q,HONG H,WU J,et al.Bioavailability of bioactive peptides derived from food proteins across the intestinal epithelial membrane:A review[J].Trends in Food Science & Technology,2019,86:399-411.
[23] 王祖哲,马普,王军琦,等.刺参低聚肽的质量评价和抗疲劳作用研究[J].食品研究与开发,2019,40(18):5-11.
WANG Z Z,MA P,WANG J Q,et al.Quality evaluation and anti-fatigue effect of Apostichopus japonicus oligopeptides[J].Food Research and Development,2019,40(18):5-11.
[24] MUKAIDA N,HARADA A,MATSUSHIMA K.Interleukin-8 (IL-8) and monocyte chemotactic and activating factor (MCAF/MCP-1),chemokines essentially involved in inflammatory and immune reactions[J].Cytokine & Growth Factor Reviews,1998,9(1):9-23.
[25] COUPER K N,BLOUNT D G,RILEY E M.IL-10:The master regulator of immunity to infection[J].The Journal of Immunology,2008,180(9):5 771-5 777.
[26] LI P,WU G.Roles of dietary glycine,proline,and hydroxyproline in collagen synthesis and animal growth[J].Amino Acids,2018,50(1):29-38.
[27] SHIMOMURA Y,KITAURA Y.Physiological and pathological roles of branched-chain amino acids in the regulation of protein and energy metabolism and neurological functions[J].Pharmacological Research,2018,133:215-217.
[28] NAPAVICHAYANUN S,ARAMWIT P.Effect of animal products and extracts on wound healing promotion in topical applications:A review[J].Journal of Biomaterials Science,Polymer Edition,2017,28(8):703-729.
[29] 张梦,樊光辉,张宜,等.云南白药在创伤中的新应用[J].中国药房,2014,25(23):2 194-2 196.
ZHANG M,FAN G H,ZHANG Y,et al.New application of Yunnan Baiyao in trauma[J].China Pharmacy,2014,25(23):2 194-2 196.
[30] JANIS J E,HARRISON B.Wound Healing[J].Plastic and Reconstructive Surgery,2014,133(2):199e-207e.
[31] EMING S A,MARTIN P,TOMIC-CANIC M.Wound repair and regeneration:Mechanisms,signaling,and translation[J].Science Translational Medicine,2014,6(265):265s-266s.
[32] WANG J,XU M,LIANG R,et al.Oral administration of marine collagen peptides prepared from chum salmon (Oncorhynchus keta) improves wound healing following cesarean section in rats[J].Food & Nutrition Research,2017,59(1):26 411.
[33] SLEMP A E,KIRSCHNER R E.Keloids and scars:A review of keloids and scars,their pathogenesis,risk factors,and management[J].Current Opinion in Pediatrics,2006,18(4):396-402.
[34] CONSTANTINO ROSA SANTOS S,MIGUEL C,DOMINGUES I,et al.VEGF and VEGFR-2 (KDR) internalization is required for endothelial recovery during wound healing[J].Experimental Cell Research,2007,313(8):1 561-1 574.
[35] CHOI J K,JANG J,JANG W,et al.The effect of epidermal growth factor (EGF) conjugated with low-molecular-weight protamine (LMWP) on wound healing of the skin[J].Biomaterials,2012,33(33):8 579-8 590.
[36] 王晓,赵静,杜梦颖,等.白芨胶载外源性碱性成纤维细胞生长因子促进伤口愈合的实验研究[J].中国组织工程研究,2017,21(34):5 481-5 486.
WANG X,ZHAO J,DU M Y,et al.Experimental research on bletilla carrying exogenous basic fibroblast growth factor that promotes wound healing[J].Chinese Journal of Tissue Engineering Research,2017,21(34):5 481-5 486.
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