综述与专题评论

基因组学及其在白酒酿造基础研究领域中的应用

  • 余晶晶 ,
  • 黄永光
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  • (贵州大学 酿酒与食品工程学院,贵州大学贵州省发酵工程与生物制药重点实验室,贵州 贵阳,550025)
硕士研究生(黄永光研究员为通讯作者,E-mail:772566120@qq.com)

收稿日期: 2020-10-20

  修回日期: 2020-11-23

  网络出版日期: 2021-05-20

基金资助

贵州省科技支撑计划项目(黔科合支撑[2017]2542);贵州省重点基金项目(黔科合基础[2017]1405);贵州省科技平台及人才团队计划项目(黔科合平台人才[2016]5637);贵州省科学技术厅重大专项(黔科合重大专项字[2015]6012);贵州省科技计划项目(黔科合[2019]2169);贵州大学引进人才项目(贵大人基合字[2016]38号)

Genomics and its application in basic research field of Baijiu brewing

  • YU Jingjing ,
  • HUANG Yongguang
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  • (Key Laboratory of Fermentation Engineering and Bio-Pharmacy of Guizhou Province,Guizhou University School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China)

Received date: 2020-10-20

  Revised date: 2020-11-23

  Online published: 2021-05-20

摘要

中国白酒酿造属于典型的多菌种固态发酵模式,因特殊的开放式酿造工艺使得参与发酵的微生物种类极其复杂,然而传统分离培养技术限制了对复杂微生物体系的深入研究。基因组学以其通量高、规模大等特点为解析复杂多样的生物体基因组提供了技术手段,能够实现对传统白酒固态发酵微生物及其表达调控的研究,最终揭示微生物与功能代谢物之间的内在关联性。本文主要综述了基因组学关键技术及其在白酒酿造基础研究中的多方面应用,总结多组学联合技术在白酒酿造基础研究中的应用现状,为实现高产优质、现代化酿造白酒的研究提供参考。

本文引用格式

余晶晶 , 黄永光 . 基因组学及其在白酒酿造基础研究领域中的应用[J]. 食品与发酵工业, 2021 , 47(7) : 278 -284 . DOI: 10.13995/j.cnki.11-1802/ts.025942

Abstract

Chinese Baijiu brewing belongs to typical multi-strain solid fermentation.Microbial species that involved in fermentation are relatively complicated due to its unique open fermentation brewing process.However, research of complex microbial system was limited by the traditional isolation and cutivation techniques.Genomics, characterized by high throughput and large scale, provides a mechanical means for the analysis of complex microbial genomes, which can realize the research on the microorganism of traditional Baijiu solid fermentation and its expression regulation and further reveal the intrinsic relationship between microorganism and functional metabolites.In this paper, the fundamental technologies of genomics, the applications of genomics and multi-omics in the basic research field of liquor-making were summarized, which provides a reference for realizing high yield and quality modern Baijiu.

参考文献

[1] ZHOU X G,REN L F,LI Y T,et al.The next-generation sequencing technology:A technology review and future perspective[J].Science China Life Sciences,2010,53(1):44-57.
[2] 邓杰,卫春会,罗惠波,等.基于高通量测序的浓香型白酒窖池古菌群落结构分析[J].现代食品科技,2015,31(8):205-210.
DENG J,WEI C H,LUO H M,et al.High-throughput sequencing of the archaeal community in cellar pit mud used in Luzhou flavor liquor production[J].Modern Food Science & Technology,2015,31(8):205-210.
[3] ZUO Q C,HUANG Y G,GUO M.Evaluation of bacterial diversity during fermentation process:A comparison between handmade and machine-made high-temperature Daqu of Maotai-flavor liquor[J].Annals of Microbiology,2020,70(1):14-23.
[4] 朱治宇,黄永光.基于高通量测序对茅台镇酱香白酒主酿区域霉菌菌群结构多样性的解析[J/OL].食品科学,2020.http://kns.cnki.net/kcms/detail/11.2206.TS.20200313.1610.044.html.
ZHU Z Y,HUANG Y G.Structural diversity analysis of mold fungi in Maotai Zhenjiu liquor based on high-throughput sequencing[J].Food Science,2020.http://kns.cnki.net/kcms/detail/11.2206.TS.20200313.1610.044.html.
[5] 罗方雯,黄永光,涂华彬,等.基于高通量测序技术对茅台镇酱香白酒主酿区域酵母菌群结构多样性的解析[J].食品科学,2020,41(20):127-133.
LUO F W,HUANG Y G,TU H B,et al.Analysis of the structural diversity of yeast community in Maotai town main brewing area of Maotai liquor based on high-throughput sequencing technology[J].Food Science,2020,41(20):127-133.
[6] 胡小霞, 黄永光,涂华彬,等.酱香型白酒1轮次酿造细菌的菌群结构[J].食品科学,2020,41(14):175-182.
HU X X,HUANG Y G,TU H B,et al.Bacterial diversity structure during the first round of pile and pit fermentation of Moutai-flavor Baijiu[J].Food Science,2020,41(14):175-182.
[7] LEWIN R.Proposal to sequence the human genome stirs debate[J].Science,1986,232(4 758):1 598-1 600.
[8] 王丛茂.高通量基因组数据的处理、分析与建模[D].上海:上海交通大学,2012.
WANG C M.Processing,analysis and modeling on high-throughput genomic data[D].Shanghai:Shanghai Jiao Tong University,2012.
[9] WU Q,PENG S Q,YU Y,et al.Genome sequence of Bacillus licheniformis CGMCC3963,a stress-resistant strain isolated in a Chinese traditional solid-state liquor-making process[J].Genome Announc,2013,1(1).DOI:10.1128/genomeA.00060-12.
[10] GUO M Y,HUO D Q,GHAI R,et al.Metagenomics of ancient fermentation pits used for the production of Chinese strong-aroma liquor[J].Genome Announcements,2014,2(5):e01045-14.
[11] TANJA,MAGO,STEVEN L,et al.FLASH:fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2 957-2 963.
[12] ANTHONY M.BOLGER,MARC L,et al.Trimmomatic:A flexible trimmer for Illumina sequence data[J].Bioinformatics,2014,30(15):2 114-2 120.
[13] 丁啸. 基于序列特征的宏基因组数据分析方法研究[D].南京:东南大学,2016.
DING X.Researches on the sequence-feature-based metagenomic data analysis methods[D].Nanjing:Southeast University,2016.
[14] ALTSCHUL S F.Basic local alignment search tool (BLAST)[J].Journal of Molecular Biology,2012,215(3):403-410.
[15] BAIROCH A,APWEILER R.The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000[J].Nucleic Acids Research,2000,28(1):45-48.
[16] MIMODRI H,JENNIFER D,JANE L,et al.The gene ontology project in 2008[J].Nucleic Acids Research,2007,36:D440-D444.
[17] TATUSOV R,GALPERIN M,NATALE D,et al.The COG database:A tool for genome-scale analysis of protein functions and evolution[J].Nucleic Acids Research,2000,28(1):33-36.
[18] KANEHISA M,GOTO S.KEGG:Kyoto encyclopedia of genes and genomes[J].Nucleic Acids Research,2000,28(1):27-30.
[19] 邓啸. 基因组拓扑网络结构模型的改进及其在B型链球菌泛基因组分析中的应用[D].苏州:苏州大学,2018.
DENG X.The updating of genome topology network and the application to the pan-genome of group B Streptococcus genomes[D].Suzhou:Soochow University,2018.
[20] JIANG J,GU J,ZHANG L,et al.Comparing Mycobacterium tuberculosis genomes using genome topology networks[J].BMC Genomics.2015,16(1):1-10.
[21] 郭静. 地衣芽孢杆菌及其α-聚谷氨酸合成调控机理的系统生物学研究[D].武汉:华中农业大学,2016.
GUO J.Investigating the Bacillus Licheniformis and the synthesis regulation mechanism of ITS α-PGA using system biology method[D].Wuhan:Huazhong Agricultural University,2016.
[22] 葛磊鑫. 基于B族维生素合成与利用特征的湖泊微生物种间关系分析[D].武汉:华中师范大学,2019.
GE L X.Analysis of microbial interspecies relationship based on the properties of B vitamins synthesis and utilization[D].Wuhan:Central China Normal University,2019.
[23] 任爱容, 黄永光.茅台镇不同主酿区域酱香型白酒酿造大曲中细菌菌群结构分析[J].食品科学,2021,42(4):167-174.
REN A R,HUANG Y G.Analysis of bacterial microflora structure in Daqu of Maotai-flavor liquor brewing in different main brewing regions in Moutai town[J].Food Science,2021,42(4):167-174.
[24] 张艳. 酱香型白酒发酵中乳酸菌群结构及功能研究[D].无锡:江南大学,2015.
ZHANG Y.Community structure and function of lactic acid bacteria during Maotai-flavor liquor fermentation[D].Wuxi:Jiangnan University,2015.
[25] WANG L,WANG Y Y,WANG D Q,et al.Dynamic changes in the bacterial community in Moutai liquor fermentation process characterized by deep sequencing[J].Journal of the Institute of Brewing,2015,121(4):603-608.
[26] 勾文君, 田源,孔小勇,等.洋河酒窖泥细菌群落结构与菌株产酸能力分析[J].微生物学通报,2020,47(6):1 651-1 661.
GOU W J,TIAN Y,KONG X Y,et al.Bacterial composition in pit mud of Yanghe liquor and identification of acid producing bacteria[J].Chinese Society for Microbiology,2020,47(6):1 651-1 661.
[27] 王雪山. 不同环境清香类型白酒发酵微生物种群结构比较及溯源解析[D].无锡:江南大学,2018.
WANG X S.Microbial community structure and microbial source tracking of Chinese light-flavor liquor fermentation in different environments[D].Wuxi:Jiangnan University,2018.
[28] 刘芸雅. 绍兴黄酒发酵中微生物群落结构及其对风味物质影响研究[D].无锡:江南大学,2015.
LIU Y Y.Characterization of microflora and their functions on flavor compounds in Shaoxing rice wine[D].Wuxi:Jiangnan University,2015.
[29] LI W W,FAN G S,FU Z L,et al.Effects of fortification of Daqu with various yeasts on microbial community structure and flavor metabolism[J].Food Research International,2019,129:108 837.
[30] 黎瑶依, 胡小霞,黄永光.茅台镇酱香型白酒酿造环境中真菌菌群多样性分析[J/OL].食品科学,2020.http://kns.cnki.net/kcms/detail/11.2206.TS.20200831.0939.012.html.
LI Y Y,HU X X,HUANG Y G.Analysis on the diversity of fungi flora in Maotai-flavor liquor brewing environment in Moutai town[J/OL].Food Science,2020.http://kns.cnki.net/kcms/detail/11.2206.TS.20200831.0939.012.html.
[31] LIU S,CHEN Q,ZOU H,et al.A metagenomic analysis of the relationship between microorganisms and flavor development in Shaoxing mechanized Huangjiu fermentation mashes[J].International Journal of Food Microbiology,2019,303:9-18.
[32] LI D N,HUANG W,WANG C X,et al.The complete genome sequence of the thermophilic bacterium Laceyella sacchari FBKL4.010 reveals the basis for tetramethylpyrazine biosynthesis in Moutai-flavor Daqu[J].Microbiologyopen,2019,8(12):11.
[33] 彭素琴, 吴群,徐岩.产酱香地衣芽孢杆菌CGMCC 3963耐受特征及基于转录组学的耐受机制分析[J].微生物学通报,2014,41(12):2 395-2 403.
PENG S Q,WU Q,XU Y.Tolerance characteristics of Bacillus licheniformis CGMCC 3963 and tolerance mechanisms based on transcriptome analysis[J].Chinese Society for Microbiology,2014,41(12):2 395-2 403.
[34] LU X,WU Q,ZHANG Y,et al.Genomic and transcriptomic analyses of the Chinese Maotai-flavored liquor yeast MT1 revealed its unique multi-carbon co-utilization[J].BMC Genomics,2015,16(1):1 064.
[35] WANG Y S,LI B,DONG H,et al.Complete genome sequence of Clostridium kluyveri JZZ applied in Chinese strong-flavor liquor production[J].Current Microbiology,2018,75(11):1 429-1 433.
[36] BOKULICH N A,BERGSVEINSON J,ZIOLA B,et al.Mapping microbial ecosystems and spoilage-gene flow in breweries highlights patterns of contamination and resistance[J].eLife,2015,4(4):4 364-4 366.
[37] WANG X,DU H,XU Y.Source tracking of prokaryotic communities in fermented grain of Chinese strong-flavor liquor[J].International Journal of Food Microbiology,2017,244:27-35.
[38] 卢建军, 杨帆,杨婧,等.白酒酿造中产正丙醇的微生物溯源研究[J].中国酿造,2019,38(7):151-155.
LU J J,YANG F,YANG J,et al.Source tracing of 1-propanol-producing microorganisms during Baijiu fermentation[J].China brewing,2019,38(7):151-155.
[39] 张伟平. 酿酒酵母氮代谢物阻遏效应系统生物学解析和全局调控[D].无锡:江南大学,2018.
ZHANG W P.System biology study and global regulation of nitrogen catabolite repression in Saccharomyces cerevisiae[D].Wuxi:Jiangnan University,2018.
[40] JACKSON,PETER,MATTEO,et al.Genome evolution across 1,011 Saccharomyces cerevisiae isolates[J].Nature,2018,556(7 701):339-344.
[41] XU Y,ZHI Y,WU Q,et al.Zygosaccharomyces bailii is a potential producer of various flavor compounds in Chinese Maotai-flavor liquor fermentation[J].Frontiers in Microbiology,2017,8:2 609.
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