食品与发酵工业 >

2021 , Vol. 47 >Issue 10: 203 - 206

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025904

分析与检测

基于染料Genefinder的荧光传感体系检测玉米赤霉烯酮

  • 郭婷 ,
  • 陈金航 ,
  • 周鸿媛 ,
  • 张宇昊 ,
  • 马良
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  • (西南大学 食品科学学院,重庆,400715)
博士,讲师(马良教授为通讯作者,E-mail:zhyhml@163.com)

收稿日期: 2020-10-20

  修回日期: 2020-12-03

  网络出版日期: 2021-06-17

基金资助

中央高校基本科研业务费专项资金(XDJK2020C052;XDJK2020B044)

收起

A fluorescent sensing system based on Genefinder for detection of zearalenone

  • GUO Ting ,
  • CHEN Jinhang ,
  • ZHOU Hongyuan ,
  • ZHANG Yuhao ,
  • MA Liang
Expand
  • (College of Food Science, Southwest University, Chongqing 400715, China)

Received date: 2020-10-20

  Revised date: 2020-12-03

  Online published: 2021-06-17

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摘要

玉米赤霉烯酮(zearalenone,ZEN)是由镰刀菌属真菌产生的一种毒素,具有生殖毒性、免疫毒性、肝肾毒性及致癌性,对人类健康造成严重威胁。该研究通过构建一种简单、灵敏的荧光传感体系检测ZEN,其中核酸适配体作为识别元件,对目标物ZEN具有特异性识别作用;Genefinder染料作为荧光传感信号,当染料与ZEN适配体及其互补链形成的双链结构作用时,染料荧光信号增强,然而加入ZEN后,适配体特异性识别ZEN,导致双链打开,染料荧光强度降低。研究结果表明,该传感体系对ZEN具有较好的选择性,在最优条件下,这种简单快速的荧光传感体系的线性范围是0.1~200 μg/L,实际检出限为0.1 μg/L。以玉米粉为实际样品,ZEN的加标回收率为100.4％~105.8％,该荧光传感体系成功用于实际样品检测,具有广阔的应用前景。

关键词： 玉米赤霉烯酮; 核酸适配体; 染料Genefinder; 荧光传感体系; 高灵敏

本文引用格式

郭婷 , 陈金航 , 周鸿媛 , 张宇昊 , 马良 . 基于染料Genefinder的荧光传感体系检测玉米赤霉烯酮[J]. 食品与发酵工业, 2021 , 47(10) : 203 -206 . DOI: 10.13995/j.cnki.11-1802/ts.025904

Abstract

Zearalenone (ZEN) is a kind of toxin produced by Fusarium fungi. It has reproductive toxicity, immunotoxicity, hepatorenal toxicity and carcinogenicity, and poses a serious threat to human health. In this work, a facile and sensitive fluorescence sensing system was developed for the detection of ZEN. Aptamer as recognition element could recognize the target ZEN. Genefinder was chosen as the sensing signal, the fluorescence signal will increase due to the interaction between Genefinder and double DNA which formed by ZEN aptamer and its complementary strand. But in the presence of ZEN, the aptamer could recognize the ZEN, leading to decrease of fluorescence. The results showed that the sensing system was high selectivity for ZEN, and the detection linear range was 0.1-200 μg/L with 0.1 μg/L the detection limit under the optimized condition. For real corn flour sample, the standard recovery ranges from 100.4% to 105.8%. The fluorescence sensing system has been successfully used for the detection of ZEN in the real sample which showed a wide application potential.

Key words： zearalenone; aptamer; Genefinder; fluorescence sensing system; high sensitive

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