为了检测影响赤霞珠干红葡萄酒苦涩感的几种酚类物质含量,采用高效液相色谱(Shimadzu LC-20AD),选用platisil ODS色谱柱(250 mm×4.6 mm,5 μm),流动相A为V(水)∶V(乙酸)=98∶2,流动相B为乙腈,流速1.0 mL/min,梯度洗脱,柱温30 ℃,检测波长280 nm,样品经乙酸乙酯萃取后液相分析,建立了一种同时检测出葡萄酒中5种黄烷-3-醇单体和2种原花青素二聚体含量的方法。7种组分的浓度与其峰面积在一定范围内有良好的线性关系,相关系数为0.997 3~0.999 9,检测限为0.008 3~0.187 5 mg/L,平均回收率在91.12%~108.84%,标准品和样品保留时间的相对标准偏差均在1%以内,峰面积的相对标准偏差均在4%以内,方法可靠。采用该方法对宁夏贺兰山东麓5个不同子产区的17款干红葡萄酒进行检测分析,结果表明,除个别酒样未检出表儿茶素没食子酸酯外,不同酒庄生产的干红葡萄酒均检出7种酚类物质,各物质中表儿茶素和原花青素B1的含量高,酚类物质总量最高的是美御酒庄2016年开庄珍藏干红葡萄酒(237.528 mg/L),总量最少的是宝源大地酒庄鼎品红赤霞珠蓝钻干红葡萄酒(127.237 mg/L)。
In order to detect the content of several phenolic substances that affect the bitterness and astringency of cabernet Sauvignon dry red wine, five flavane-3-alcohol monomers and two proanthocyanidin dimers in wine were measured by HPLC(Shimadzu LC-20AD) after extraction with ethyl acetate. The detection conditions were as follows: chromatogram column: Platisil ODS(250 mm×4 mm,5 μm), flow rate 1.0 mL/min, column temperature 30℃, wavelength 280 nm, gradient elute, solvent A: V(water)∶V(acetic acid)=98∶2, solvent B: acetonitrile. The concentration of these seven components had good linear relationships with their peak areas within a certain range, the correlation coefficient was between 0.997 3-0.999 9, the detection limit was between 0.008 3-0.187 5 mg/L, and the average recovery was between 91.12%-108.84%. The relative standard deviation of the retention time of standard and sample was within 1%, and that of the peak area was within 4%. Seven phenolic substances were detected in all dry red wines from five different seed regions of the Eastern Foot of Helan Mountain in Ningxia, epigallocatechin was not detected in some wine samples. The contents of epicatechin and procyanidin B1 were higher than other substances. The highest total phenol content was 237.528 mg/L, and the lowest was 127.237 mg/L.
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