食品与发酵工业 >

2021 , Vol. 47 >Issue 11: 1 - 7

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026159

研究报告

组胺表面分子印迹聚合物的制备及其在酸奶中的应用

  • 李欣培 ,
  • 王秀君 ,
  • 徐斐 ,
  • 曹慧 ,
  • 袁敏 ,
  • 喻东威 ,
  • 刘振民
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  • 1(上海理工大学 医疗器械与食品学院,上海食品快速检测工程技术研究中心,上海,200093)
    2(山东黄金矿业股份有限公司,山东 济南,250014)
    3(内蒙古蒙牛乳业集团股份有限公司,内蒙古 呼和浩特,011500)
    4(光明乳业股份有限公司乳业研究院,上海,200436)
硕士研究生(袁敏副教授为通讯作者,E-mail: yuanmin1371@126.com)

收稿日期: 2020-11-12

  修回日期: 2021-02-03

  网络出版日期: 2021-07-16

基金资助

十三五国家重点研发计划项目(2018YFC1604202)

收起

Preparation of histamine surface molecularly imprinted polymer and its application in yoghurt

  • LI Xinpei ,
  • WANG Xiujun ,
  • XU Fei ,
  • CAO Hui ,
  • YUAN Min ,
  • YU Dongwei ,
  • LIU Zhenmin
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  • 1(School of Medical Instruments and Food Engineering,Shanghai Engineering Research Center for Food Rapid Detection,University of Shanghai for Science and Technology,Shanghai 200093,China)
    2(Shandong Gold Mining Co.Ltd.,Jinan 250014,China)
    3(Inner Mongolia MengNiu Dairy Products Co.Ltd,Hohhot 011500,China)
    4(Dairy Research Institute,Bright Dairy and Food Co.Ltd.,Shanghai 200436,China)

Received date: 2020-11-12

  Revised date: 2021-02-03

  Online published: 2021-07-16

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摘要

组胺广泛存在于发酵食品中,当人体误食高含量组胺食物时会引起严重过敏反应,因此需要建立对食品中组胺进行吸附与去除的方法。以组胺为模板分子、甲基丙烯酸为功能单体、二甲基丙烯酸乙二醇酯为交联剂,按1:10:40的体积比通过表面聚合方法在二氧化硅表面制备组胺分子印迹聚合物,并进行了吸附动力学和等温吸附表征。结果表明,该印迹聚合物对组胺具有优异的吸附性能,吸附容量高达13.1 mg/g,印迹因子达到4。相对于组氨酸、酪胺、苯乙胺等结构类似物,该印迹聚合物亦对组胺展现了良好的特异性吸附能力,其吸附容量是组氨酸的13倍。该印迹聚合物已成功应用于酸奶中组胺的吸附与去除。

关键词： 表面聚合; 分子印迹; 组胺; 高特异性吸附

本文引用格式

李欣培 , 王秀君 , 徐斐 , 曹慧 , 袁敏 , 喻东威 , 刘振民 . 组胺表面分子印迹聚合物的制备及其在酸奶中的应用[J]. 食品与发酵工业, 2021 , 47(11) : 1 -7 . DOI: 10.13995/j.cnki.11-1802/ts.026159

Abstract

Histamine usually exists in fermented food. However, it may cause serious allergic reaction if people intake food with high content of histamine. Therefore, it is necessary to establish a method for the adsorption and removal of histamine in food. In this study, a histamine molecularly imprinted polymer (MIP) on silica surface was prepared by surface polymerization. Histamine was used as the template molecule, methacrylic acid as functional monomer, and ethylene glycol dimethacrylate as crosslinker with a ratio of 1∶10∶40. The property of prepared polymers was characterized by adsorption kinetics and isothermal adsorption experiment. The results showed that the prepared MIP had good adsorption to histamine with a highest adsorption capacity of 13.1 mg/g, and the imprint factor reached 4. The prepared MIP also had an excellent specific adsorption to histamine in comparison with histidine, tyramine and phenylethylamine. The imprinted polymer was successfully applied to adsorb and remove histamine in yoghurt.

Key words： surface polymerization; molecular imprinting polymer; histamine; high specific adsorption

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