食品与发酵工业 >

2021 , Vol. 47 >Issue 11: 208 - 215

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026327

生产与科研应用

竹笋加工剩余物中笋篼黄酮提取、结构鉴定及生物活性研究

  • 杨波 ,
  • 益莎 ,
  • 施锴芸 ,
  • 李琴 ,
  • 杨光 ,
  • 贺亮
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  • 1(上海理工大学 医疗器械与食品学院,上海,200931)
    2(国家林业局竹笋工程技术研究中心,浙江省林业科学研究院,浙江省竹类研究重点实验室,浙江 杭州,310231)
副研究员(通讯作者,E-mail:yangbo6899@126.com)

收稿日期: 2020-12-03

  修回日期: 2021-01-01

  网络出版日期: 2021-07-16

基金资助

竹笋加工剩余物增值关键技术及功能产品研发(2019F1065-12)

收起

Extraction, structural identification and biological activity study of flavonoids from bamboo shoot processing residue (BSDF)

  • YANG Bo ,
  • YI Sha ,
  • SHI Kaiyun ,
  • LI Qin ,
  • YANG Guang ,
  • HE Liang
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  • 1(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200931,China)
    2(Bamboo Shoots Engineering Research Center of the State Forestry Bureau,Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Bamboo Shoot Research,Hangzhou 310231,China)

Received date: 2020-12-03

  Revised date: 2021-01-01

  Online published: 2021-07-16

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摘要

为了探索黄酮的降糖作用,以竹笋加工剩余物中笋篼为原料提取黄酮类物质,分析乙醇体积分数、料液比、提取时间、提取温度等对笋篼黄酮(flavonoids from bamboo shoot processing residue,BSDF)提取率的影响,通过响应面设计优化提取工艺,确定最佳提取条件:乙醇体积分数55%、液料比27:1 (mL:g)、提取时间66 min,此条件下最优提取率为0.33%。液质联用分析结果表明,BSDF中碳苷类物质主要有4种:夏佛塔苷、白杨素-6-C-阿拉伯糖-8-C-葡萄糖苷、芹菜素-6,8-C-二那阿拉伯糖苷、白杨素-6-C-β-D-葡萄糖苷-8-C-α-L-阿拉伯糖苷。BSDF体外活性研究结果表明,低、中、高浓度的BSDF均对人肝癌细胞HepG2有抑制作用,且随着浓度增加而增强,证明BSDF具有一定的抗肿瘤活性;建立了HepG2细胞胰岛素(insulin,IR)抵抗模型(HepG2-IR)。研究BSDF的降血糖活性,结果表明,BSDF作用于HepG2-IR细胞(15 μg/mL胰岛素诱导),在400 μg/mL时葡萄糖消耗量达到(3.51±0.28)mmol/L,大于胰岛素抵抗模型组,小于二甲双胍阳性对照组。证明BSDF可改善高浓度胰岛素诱导的HepG2细胞葡萄糖消耗量,具有降糖作用。

关键词： 竹笋加工剩余物; 笋篼; 黄酮; 抗肿瘤活性; 降血糖活性

本文引用格式

杨波 , 益莎 , 施锴芸 , 李琴 , 杨光 , 贺亮 . 竹笋加工剩余物中笋篼黄酮提取、结构鉴定及生物活性研究[J]. 食品与发酵工业, 2021 , 47(11) : 208 -215 . DOI: 10.13995/j.cnki.11-1802/ts.026327

Abstract

In this study, flavonoids were extracted from bamboo shoot processing residue (BSDF) using alcohol. The extraction rate of total flavonoids was taken as the response to analyzing the influences of ethanol concentration, solid-liquid ratio and extraction time on the extraction effect. Then, the extraction process of BSDF was optimized by response surface to determine the optimal extraction conditions,which were 55% ethanol, material-to-liquid ratio of 27∶1 (mL∶g) extraction for 66 min and the optimal extraction rate was 0.33%. The results of liquid chromatography-mass spectrometry (UPLC-Triple TOF/MS) showed that there were four main types of carbon glycosides in BSDF which were schaftoside, chrysin 6-C-arabinoside 8-C-glucoside, apigenin-6,8-C-arabinoside, chrysin 6-C-β-D-glucoside-8-C-α-L-arabinoside. Moreover, in vitro biological activity of BSDF showed that low, medium and high concentrations of BSDF had inhibitory effects on human hepatoma (HepG2), which were enhanced with the increase of BSDF concentration. It was proved that BSDF had certain anti-tumor activity. Meanwhile, the model of HepG2 cells insulin resistance (HepG2-IR) was established to detect the hypoglycemic activity of BSDF extracts in vitro. Results showed that when BSDF extract on HepG2-IR cells (15 μg/mL insulin-induced) in 400 μg/mL, the glucose consumption were (3.51±0.28) mmol/L. It was greater than insulin treatment group and less than metformin treatment group. This indicates that BSDF extract can improve the glucose consumption of HepG2-IR cells.

Key words： bamboo shoots processing residue; bamboo shoots roots; flavonoids; antitumor activity; hypoglycemic activity

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