综述与专题评论

荧光探针在高通量筛选技术上的应用进展

  • 刘宜嵩 ,
  • 王壬 ,
  • 钮成拓 ,
  • 郑飞云 ,
  • 王金晶 ,
  • 李崎 ,
  • 刘春凤
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  • 1(江南大学 生物工程学院,工业生物技术教育部重点实验室,江苏 无锡,214222)
    2(江南大学,酿酒科学与工程研究室,江苏 无锡,214222)
硕士研究生(刘春凤高级实验师为通讯作者,E-mail:liuchunfeng@jiangnan.edu.cn)

收稿日期: 2020-09-17

  修回日期: 2020-10-27

  网络出版日期: 2021-07-16

基金资助

国家自然科学基金(31601558)

Application of fluorescence probe in high throughput screening

  • LIU Yisong ,
  • WANG Ren ,
  • NIU Chengtuo ,
  • ZHENG Feiyun ,
  • WANG Jinjing ,
  • LI Qi ,
  • LIU Chunfeng
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  • 1(Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China)
    2(Lab of Brewing Science and Technology,School of Biotechnology,Jiangnan University,Wuxi 214122,China)

Received date: 2020-09-17

  Revised date: 2020-10-27

  Online published: 2021-07-16

摘要

荧光探针是一种分析检测工具,针对特定的化学物质可以将微观的识别信息转化为宏观的荧光信号,具有高灵敏度、高选择性、检测体系干扰小、操作简便等优点。基于荧光探针的优势,其在高通量筛选技术领域有着重要的应用。该文主要综述了荧光探针的起源、作用机理以及国内外关于荧光探针在高通量筛选技术上的应用进展,旨在为拓宽荧光探针技术的应用领域,推动高通量筛选技术发展提供参考。

本文引用格式

刘宜嵩 , 王壬 , 钮成拓 , 郑飞云 , 王金晶 , 李崎 , 刘春凤 . 荧光探针在高通量筛选技术上的应用进展[J]. 食品与发酵工业, 2021 , 47(11) : 266 -271 . DOI: 10.13995/j.cnki.11-1802/ts.025699

Abstract

A fluorescent probe is an analytical tool that converts microscopic recognition information into macroscopic fluorescence signals for specific chemicals.The utility model has the advantages of high sensitivity,high selectivity,little interference to the detection system and simple operation,etc.Based on the advantages of fluorescence probe,it has important application value in the field of high throughput screening technology.This article mainly summarizes the origin,mechanism of action of fluorescent probes and the application progress of fluorescent probes in high-throughput screening technology at home and aboard,aiming to expand the application field of fluorescent probe technology and provide references for the development of high-throughput screening technology.

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