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β-葡萄糖苷酶活性稳定化技术在柑橘产品增香中的应用

  • 张晨 ,
  • 贾蒙 ,
  • 马亚琴
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  • (西南大学柑桔研究所,国家柑桔工程技术研究中心,重庆,400712)
硕士研究生(马亚琴副研究员为通讯作者,E-mail: maya211@163.com)

收稿日期: 2020-10-20

  修回日期: 2020-11-03

  网络出版日期: 2021-07-16

基金资助

国家现代农业柑橘产业技术体系(CARS-26-06B);中央高校基本业务费专项资金资助(XDJK2019B048)

Application of β-glucosidase activity stabilization technology in citrus flavor enhancement

  • ZHANG Chen ,
  • JIA Meng ,
  • MA Yaqin
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  • (Citrus Research Institute,Southwest University,National Citrus Engineering Research Center,Chongqing 400712,China)

Received date: 2020-10-20

  Revised date: 2020-11-03

  Online published: 2021-07-16

摘要

柑橘加工产品的香气是衡量其品质好坏的评判指标之一,其果实中以键合态形式存在的香气前体物质和柑橘类苦味物质是影响产品香气的直接因素。应用β-葡萄糖苷酶不仅可以水解键合态香气物质以促进香气成分的释放,而且可在鼠李糖苷酶的协同作用下将柚皮苷水解成无苦味的柚皮素来减少产品苦味的形成,进而提升人们对香气的感知能力。但在实际增香环境中,β-葡萄糖苷酶活性并不稳定,各种内外因素都极大地抑制了增香效果。该文综述了目前提高β-葡萄糖苷酶在柑橘加工所处复杂环境中活性稳定化技术方面的研究现状,以期为β-葡萄糖苷酶更好地应用到柑橘加工产品中提供参考。

本文引用格式

张晨 , 贾蒙 , 马亚琴 . β-葡萄糖苷酶活性稳定化技术在柑橘产品增香中的应用[J]. 食品与发酵工业, 2021 , 47(11) : 303 -309 . DOI: 10.13995/j.cnki.11-1802/ts.025919

Abstract

The aroma of citrus processing products is one of the evaluation indexes to measure the quality of citrus products. The aroma precursors and citrus bitter substances existing in the form of bond state are the direct factors affecting the aroma of citrus products. The application of β-glucosidase can not only hydrolyze the bonded aroma substances to promote the release of aroma components, but also hydrolyze naringin into naringenin without bitterness under the synergistic effect of rhamnosidase to reduce the formation of bitterness of products, and then improve people's perception of aroma. However, the activity of β-glucosidase was not stable in the actual environment, which was greatly inhibited by various internal and external factors. In this regard, this paper summarizes the current situation of how to improve the stability of β-glucosidase activity in the complex environment of citrus processing, in order to provide reference for the better application of β-glucosidase in citrus processing products.

参考文献

[1] XU Q,CHEN L L,RUAN X A,et al.The draft genome of sweet orange (Citrus sinensis)[J].Nature Genetics,2013,45(1):59-66.
[2] 国家统计局.中国统计年鉴-主要农产品产量[M].中国统计出版社,北京数通电子出版社,2019.
National Bureau of statistics.China Statistical Yearbook-output of major agricultural products [M].China Statistics Press:Beijing Shutong Electronic Publishing House,2019.
[3] 张放.2018年中国主要柑桔加工品进出口统计简析[J].中国果业信息,2019,36(12):18-31.
ZHANG F.Statistical analysis of China's main citrus processed products import and export in 2018[J].China Fruit News,2019,36(12):18-31.
[4] RODRIGUES P D O,SANTOS B V D,COSTA L,et al.Xylanase and β-glucosidase production by Aspergillus fumigatus using commercial and lignocellulosic substrates submitted to chemical pre-treatments[J].Industrial Crops and Products,2017,95(1):453-459.
[5] ALBERTO G P,WINAI S,PAU L K.The effects of high hydrostatic pressure on β-glucosidase,peroxidase and polyphenoloxidase in red raspberry (Rubus idaells) and strawberry (Fragaria×ananassa)[J].Food Chemistry,2004,88(1):7-10.
[6] 周林芳,江波,张涛,等.糖苷水解酶第3家族β-葡萄糖苷酶的研究进展[J].食品工业科技,2017,38(14):340-345.
ZHOU L F,JIANG B,ZHANG T,et al.Research progress of β-glucosidases of glycoside hydrolase family 3[J].Science and Technology of Food Industry,2017,38(14):340-345.
[7] FRANCIS M J O,ALLCOCK C.Geraniol β-d-glucoside;occurrence and synthesis in rose flowers[J].Phytochemistry,1969,8(8):1 339-1 347.
[8] WINTERHALTER P,SCHREIER P.Biotechnology:Challenge for the Flavour Industry.In:Acree TE,Treanishi R(eds)Flavour science.ACS,Washington,1993:225
[9] 高倩. β-葡萄糖苷酶的提取纯化及其改善廉江红橙果汁风味的研究[D].湛江:广东海洋大学,2017.
GAO Q.Extraction and purification of β-glucosidase and its improvement on the flavor of red-orange juice abstract[D].Zhanjiang:Guangdong Ocean University,2017.
[10] 盛金凤,何雪梅,孙健,等.柚皮苷酶脱苦工艺优化及对金桔汁品质的影响[J].食品工业,2019,40(7):126-131.
SHENG J F,HE X M,SUN J,et al,Optimization of naringinase debittering and effect on nutritional quality of kumquat juice[J].The Food Industry,2019,40(7):126-131.
[11] STROPPIANO M,CALEVO M G,CORSOLINI F,et al.Validity of b-d-glucosidase activity measured in dried blood samples for detection of potential Gaucher disease patients[J].Clinical Biochemistry Ottawa Then New York Pergamon Press Elsevier Science,2014,47(13-14):1 293-1 296.
[12] CHAN A K N,NG A K L,NG K K Y,et al.Cloning and characterization of two novel β-glucosidase genes encoding isoenzymes of the cellobiase complex from Cellulomonas biazotea[J].Gene,2018,642:367-375.
[13] 陈守文,陈九武,赵山.利用黑曲霉β-葡萄糖苷酶改善葡萄酒的风味[J].中国酿造,1999,18(3):17-19.
CHEN S W,CHEN J W,ZHAO S.Improving wine flavor by Aspergillus niger β-glucosidase[J].China Brewing,1999,18(3):17-19.
[14] ROBINSON D.The fluorimetric determination of β-glucosidase:Its occurrence in the tissues of animals,including insects[J].The Biochemical Journal,1956,63(1):39-44.
[15] SERDIUK I,RESZKA M,MYSZKA H,et al.Flavonol-based fluorescent indicator for determination of β-glucosidase activity[J].RSC Advances,2016,6(48):42 532-42 536.
[16] CAMERON R G,MANTHEY J A,BAKER R A,et al.Purification and characterization of a beta-glucosidase from Citrus sinensis var.valencia fruit tissue[J].Journal of Agricultural & Food Chemistry,2001,49(9):4 457-4 462.
[17] WANG L W,MA J M,CHENG X,et al.Determination of β-glucosidase activity based on enzyme-triggered click chemistry[J].Chinese Journal of Organic Chemistry,2018,38(10):2775.
[18] 罗小娟,杨娟,陈锦权,等.鲍鱼脏器β-葡萄糖苷酶提取工艺的优化[J].安徽农学通报,2016,22(18):16-19;48.
LUO X J,YANG J,CHEN J Q,et al.Purification of β-glucosidase from abalone viscera and its enzymological character[J].Anhui Agricultural Science Bulletin,2016,22(18):16-19;48.
[19] BARBAGALLO R N,PALMERI R,FABIANO S,et al.Characteristic of β-glucosidase from Sicilian blood oranges in relation to anthocyanin degradation[J].Enzyme & Microbial Technology,2007,41(5):570-575.
[20] BHALLA T C,ASIF M,SMITA K.Purification and characterization of cyanogenic β-glucosidase from wild apricot (Prunus armeniaca L.)[J].Process Biochemistry,2017,58:320-325.
[21] HATIBE ERTURK KARA,SINAN S,TURAN Y.Purification of beta-glucosidase from olive (Olea europaea L.) fruit tissue with specifically designed hydrophobic interaction chromatography and characterization of the purified enzyme[J].Journal of Chromatography.B,Analytical Technologies in the Biomedical and Life Sciences,2011,879(19):1 507-1 512.
[22] ABDELLA A,EL-BAZ A F,IBRAHIM I A,et al.Biotransformation of soy flour isoflavones by Aspergillus niger NRRL 3122 β-glucosidase enzyme[J].Natural Product Research,2017,32(2):1-10.
[23] KUO H P,WANG R,HUANG C Y,et al.Characterization of an extracellular β-glucosidase from Dekkera bruxellensis for resveratrol production[J].Journal of Food & Drug Analysis,2018,26(1):163-171.
[24] KO J A,PARK J Y,KWON H J,et al.Purification and functional characterization of the first stilbene glucoside-specific β-glucosidase isolated from Lactobacillus kimchi[J].Enzyme and Microbial Technology,2014,67(12):59-66.
[25] FANSI K,JINGWEN Y,ZHEN Z,et al.Gene cloning and molecular characterization of a β-glucosidase from Thermotoga naphthophila RUK-10:An effective tool for synthesis of galacto-oligosaccharide and alkyl galactopyranosides[J].Chemical Research in Chinese University,2015,31(5):774-780.
[26] 王松林,彭荣,崔榕,等.类柠檬苦素生物转化与脱苦研究进展[J].食品科学,2015,36(9):279-283.
WANG S L,PENG R,CUI R,et al.Biotransformation and debittering of limonoids:An overview[J].Food Science,2015,36(9):278-283.
[27] 晏敏,周宇,贺肖寒,等.柑橘籽中柠檬苦素及类似物的生物活性研究进展[J].食品与发酵工业,2018,44(2):290-296.
YAN M,ZHOU Y,HE X H,et al.Research progress on the bioactivity of limonin and its analogues in Citrus[J].Food and Fermentation Industries,2018,44(2):290-296.
[28] MCINTOSH C A,MANSELL R L.Distribution of limonin during the growth and development of leaves and branches of Citrus paradisi[J].Journal of Agricultural & Food Chemistry,1983,31(2):319-325.
[29] 姚辉.产柚苷酶菌株的选育及柚子汁酶法脱苦工艺的研究[D].福州:福建农林大学,2009.
YAO H.Screening and mutation breeding of the naringinase producing strain and the study of the debittering technology by naringinase[D].Fuzhou:Fujian Agriculture and Forestry University,2009.
[30] 张阳,江璐,郭志君,等.利用β-葡萄糖苷酶提高葡萄酒香气的研究进展[J].现代食品科技,2020,36(4):316-324.
ZHANG Y,JIANG L,GUO Z J,et al.Improving wine aroma by using beta-glucosidase:A review[J].Modern Food Science and Technology,2020,36(4):316-324.
[31] AGRAWAL R,VERMA A K,SATLEWAL A.Application of nanoparticle-immobilized thermostable β-glucosidase for improving the sugarcane juice properties[J].Innovative Food Science & Emerging Technologies,2016,33(2):472-482.
[32] ÜNAL MÜ,ŞENER A.Biochemical properties of β-glucosidase from Turkish Hacıhaliloğlu apricot (Prunus armenica L.) as affected by harvest year[J].LWT-Food Science and Technology,2017,79(6):190-196.
[33] LIU R,ZHANG Q H,CHEN F S,et al.Analysis of culturable yeast diversity in spontaneously fermented orange wine,orange peel and orangery soil of a Ponkan plantation in China[J].Annals of Microbiology,2015,65(4):2 387-2 391.
[34] BELDA I,RUIZ J,ALASTRUEY-IZQUIERDO A,et al.Unraveling the enzymatic basis of wine “Flavorome”:A phylo-functional study of wine related yeast species[J].Frontiers in Microbiology,2016,7(1):12.
[35] SABEL A,MARTENS S,PETRI A,et al.Wickerhamomyces anomalus AS1:A new strain with potential to improve wine aroma[J].Annals of Microbiology,2014,64(2):483-491.
[36] HU L,LIU R,WANG X,ZHANG X.The sensory quality improvement of citrus wine through Co-Fermentations with selected non-saccharomyces yeast strains and Saccharomyces cerevisiae[J].Microorganisms,2020,8(3):323.
[37] HU L L,WANG J,JI X A,et al.Selection of non-Saccharomyces yeasts for orange wine fermentation based on their enological traits and volatile compounds formation[J].Journal of Food Science and Technology,2018,55(10):4 001-4 012.
[38] MARIA A V,JUAN U I,ANA B P.Enhancement of aroma in white wines using a β-glucosidase preparation from Debaryomyces pseudopolymorphus (A-77)[J].Food Biotechnology,2007,21(2):181-194.
[39] BAFFI M A,TOBAL T,LAGO J H G,et al.Wine aroma improvement using a β-glucosidase preparation from Aureobasidium pullulans[J].Applied Biochemistry & Biotechnology,2013,169(2):493-501.
[40] ZHOU Y,PAN S,WU T,et al.Optimal immobilization of β-glucosidase into chitosan beads using response surface methodology[J].Electronic Journal of Biotechnology,2013,16(6):6.
[41] BODAKOWSKA-BOCZNIEWICZ J,GARNCAREK Z.Immobilization of naringinase from Penicillium decumbens on chitosan microspheres for debittering grapefruit juice[J].Molecules,2019,24(23):4 234.
[42] PAULA GONZÁLEZ-POMBO,FARI?A L,CARRAU F,et al.A novel extracellular β-glucosidase from Issatchenkia terricola:Lsolation,immobilization and application for aroma enhancement of white Muscat wine[J].Process Biochemistry,2011,46(1):385-389.
[43] ZHANG Y,MIN Z,QIN Y,et al.Efficient display of Aspergillus niger β-glucosidase on Saccharomyces cerevisiae cell wall for aroma enhancement in wine[J].Journal of Agricultural and Food Chemistry,2019,67(18):5 169-5 176.
[44] KAYA M,ITO J,KOTAKA A,,et al.Isoflavone aglycones production from isoflavone glycosides by display of β-glucosidase from Aspergillus oryzae on yeast cell surface[J].Applied Microbiology and Biotechnology,2008,79(1):51-60.
[45] SAUER M,STEIGER M,MATTANOVICH D,et al.Bioprocessing of Renewable Resources to Commodity Bioproducts[M].Bisaria VS,2014.
[46] 王佳堃,孙中远,刘建新.酵母细胞表面展示技术[J].动物营养学报,2011,23(11):1 847-1 853.
WANG J K,SUN Z Y,LIU J X.Recent advances in yeast cell-surface display technology[J].Chinese Journal of Animal Nutrition,2011,23(11):1 847-1 853.
[47] FANG W,YANG Y,ZHANG X,et al.Improve ethanol tolerance of β-glucosidase Bgl1A by semi-rational engineering for the hydrolysis of soybean isoflavone glycosides[J].Journal of Biotechnology,2016,227:64-71.
[48] GIUSEPPE P O D,SOUZA T D A C B,SOUZA F H M,et al.Structural basis for glucose tolerance in GH1 β-glucosidases[J].Acta Crystallographica Section D,Biological Crystallography,2014,70(Pt 6):1 631-1 639.
[49] KONAR S,SINHA SK,DATTA S,et al.Probing the effect of glucose on the activity and stability of β-glucosidase:An all-atom molecular dynamics simulation investigation[J].ACS omega,2019,4(6):11 189-11 196.
[50] SINHA SK,DAS S,KONAR S,et al.Elucidating the regulation of glucose tolerance in a β-glucosidase from Halothermothrix orenii by active site pocket engineering and computational analysis[J].International Journal of Biological Macromolecules,2020,156:621-632.
[51] 杨芮,吕珍,文彦,等.酒类酒球菌中β-葡萄糖苷酶性质研究[J].食品科学,2013,34(23):206-211.
YANG R,LYU Z,WEN Y,et al.β-glycosidase activity of Oenococcus oeni[J].Food Science,2013,34(23):206-211.
[52] DOUKYU N,OGINO H.Organic solvent-tolerant enzymes[J].Biochemical Engineering Journal,2010,48(3):270-282.
[53] KARNAOURI A,TOPAKAS E,PASCHOS T,et al.Cloning,expression and characterization of an ethanol tolerant GH3 β-glucosidase from Myceliophthora thermophila[J].Peerj,2013,1(1):e46.
[54] MAO X X,HONG Y Z,SHAO Z Z,et al.A novel cold-active and alkali-stable β-glucosidase gene isolated from the marine bacterium martelella mediterranea[J].Applied Biochemistry and Biotechnology,2010,162(8):2 136-2 148.
[55] MIAO L L,HOU Y J,FAN H X,et al.Molecular structural basis for the cold-adaptedness of the psychrophilic β-glucosidase BglU in Micrococcus antarcticus[J].Applied & Environmental Microbiology,2016,82(7):2 021-2 030.
[56] SOUZA V P,CECÍLIA M,IKEGAMI,et al.Protein thermal denaturation is modulated by central residues in the protein structure network[J].The FEBS Journal,2016,283(6):1 124-1 138.
[57] SUN J J,WANG W,YAO Y,et al.Overexpression and characterization of a novel cold-adapted and salt-tolerant GH1 β-glucosidase from the marine bacterium Alteromonas sp.L82[J].Journal of Microbiology,2018,56(9):656-664.
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