食品与发酵工业 >

2021 , Vol. 47 >Issue 12: 83 - 89

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026920

研究报告

茅莓根提取物的体外抗氧化活性和α-葡萄糖苷酶、乙酰胆碱酯酶抑制能力研究

  • 林紫兰 ,
  • 沙小梅 ,
  • 张志斌 ,
  • 夏其乐 ,
  • 孙蕊 ,
  • 王振兴
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  • 1(西南林业大学 林学院,云南 昆明,650224)
    2(江西师范大学 生命科学学院,江西 南昌,330022)
    3(浙江省农业科学院 食品科学研究所,浙江 杭州,310021)
    4(西南山地森林资源保育与利用教育部重点实验室(西南林业大学),云南 昆明,650224)
    5(西南林业大学 生命科学学院,云南 昆明,650224)
硕士研究生(孙蕊讲师和王振兴助理研究员为共同通讯作者,E-mail:sunruithree@126.com;wangzhenxingfood@163.com)

收稿日期: 2021-01-29

  修回日期: 2021-02-16

  网络出版日期: 2021-07-22

基金资助

国家自然科学基金地区科学基金(31960078);云南省教育厅科学研究基金(2016ZZX150)

收起

In vitro antioxidant activities and inhibitory activities of α-glucosidase and acetylcholinesterase in Rubus parvifolius L. root extract

  • LIN Zilan ,
  • SHA Xiaomei ,
  • ZHANG Zhibin ,
  • XIA Qile ,
  • SUN Rui ,
  • WANG Zhenxing
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  • 1(College of Forestry, Southwest Forestry University, Kunming 650224, China)
    2(College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China)
    3(Zhejiang Academy of Agricultural Sciences, Food Science Institute, Hangzhou 310021, China)
    4(Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China,Ministry of Education,
    Southwest Forestry University, Kunming 650224, China)
    5(College of Life Sciences, Southwest Forestry University, Kunming 650224, China)

Received date: 2021-01-29

  Revised date: 2021-02-16

  Online published: 2021-07-22

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摘要

为进一步深入了解茅莓根的功能活性,以茅莓根为原材料,利用超声波辅助甲醇溶液提取制备茅莓根提取物(Rubus parvifolius L.root extract,RRE)。测定RRE中的总酚、总黄酮含量,采用DPPH自由基、ABTS阳离子自由基、超氧阴离子自由基清除能力和铁还原能力评价其体外抗氧化能力,并利用荧光光谱法和紫外光谱法测定其α-葡萄糖苷酶抑制能力和乙酰胆碱酯酶抑制能力。结果如下,RRE的总酚含量为446.392 mg GAE/g,总黄酮含量为638.337 mg Rutin/g;RRE的DPPH自由基清除能力为871.878 mg Trolox/g,ABTS阳离子自由基清除能力为1 177.854 mg Trolox/g,超氧阴离子自由基清除能力为419.928 mg Trolox/g,铁还原能力为1 827.378 mg FeSO4/g;RRE抑制α-葡萄糖苷酶的IC50值为1.314 mg/mL,抑制乙酰胆碱酯酶的IC50值为403.347 μg/mL。说明RRE具有一定的体外抗氧化能力和抑制α-葡萄糖苷酶和乙酰胆碱酯酶的能力,具有开发成天然抗氧化剂、降血糖和防止老年痴呆药物的潜力。该研究可为茅莓资源的开发利用提供科学依据。

关键词： 茅莓根; 提取物; 体外抗氧化; α-葡萄糖苷酶抑制能力; 乙酰胆碱酯酶抑制能力

本文引用格式

林紫兰 , 沙小梅 , 张志斌 , 夏其乐 , 孙蕊 , 王振兴 . 茅莓根提取物的体外抗氧化活性和α-葡萄糖苷酶、乙酰胆碱酯酶抑制能力研究[J]. 食品与发酵工业, 2021 , 47(12) : 83 -89 . DOI: 10.13995/j.cnki.11-1802/ts.026920

Abstract

To further investigate the function of Rubus parvifolius L. Root, an ultrasound-assisted methanol extraction assay was used to prepare Rubus parvifolius L. extract (RRE). The total phenolic content (TPC) and total flavonoid content (TFC) were determined, and the in vitro antioxidant activity was also assessed by measuring DPPH, ABTS, and superoxide anions radical scavenging abilities, as well as ferric reducing antioxidant power. In addition, the α-glucosidase and acetylcholinesterase inhibition activity of RRE were evaluated using fluorescence spectroscopy and UV-VIS spectrometry. The results showed that the TPC and TFC of RRE were 446.392 mg GAE/g and 638.337 mg Rutin/g. RRE had the antioxidant activities with values of 871.878 mg Trolox/g, 1 177.854 mg Trolox/g, 419.928 mg Trolox/g, and 1 827.378 mg FeSO4/g for DPPH, ABTS, superoxide anions radical scavenging abilities, and ferric reducing antioxidant power, respectively, and also inhibited α-glucosidase and acetylcholinesterase with the IC50 values of 1.314 mg/mL and 403.347 μg/mL. Taken together, RRE had certain in vitro antioxidant, α-glucosidase and acetylcholinesterase inhibition capacities, and could be developed as natural antioxidants, hypoglycemic and anti-dementia drugs. This research can provide scientific evidence for the development and utilization of Rubus parvifolius L.

Key words： Rubus parvifolius L.Root; extract; in vitro antioxidation; α-glucosidase inhibition; acetylcholinesterase inhibition

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