食品与发酵工业 >

2021 , Vol. 47 >Issue 13: 43 - 50

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026564

研究报告

产胞外多糖植物乳杆菌WHH589的免疫活性及其在发酵乳中的应用

  • 孙盛 ,
  • 陈作国 ,
  • 俞赟霞 ,
  • 曲冬梅 ,
  • 余腾斐 ,
  • 李言郡 ,
  • 陈苏
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  • (杭州娃哈哈集团有限公司,杭州娃哈哈科技有限公司,浙江省食品生物工程重点实验室,浙江 杭州,310018)
硕士,中级工程师(陈苏高级工程师为通讯作者,E-mail: chensu@wahaha.com.cn)

收稿日期: 2021-01-08

  修回日期: 2021-01-28

  网络出版日期: 2021-08-02

基金资助

国际合作技术研发与示范推广项目-产细菌素优质乳酸菌的精准筛选及产业化关键技术(2020C04002)

收起

The immunomodulatory activity of Lactobacillus plantarum WHH589 with extracellular polysaccharide-producing property and its application in fermented milk

  • SUN Sheng ,
  • CHEN Zuoguo ,
  • YU Yunxia ,
  • QU Dongmei ,
  • YU Tengfei ,
  • LI Yanjun ,
  • CHEN Su
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  • (Key Laboratory of Food and Biological Engineering of Zhejiang Province,Hangzhou Wahaha Technology Co.Ltd.,Hangzhou Wahaha Group Co.Ltd.,Hangzhou 310018,China)

Received date: 2021-01-08

  Revised date: 2021-01-28

  Online published: 2021-08-02

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摘要

从我国传统发酵食品中分离、筛选高产胞外多糖的乳酸菌,对乳酸菌的基础特性和免疫活性进行研究,并探索其在发酵乳中的应用。采用菌落拉丝法和硫酸-苯酚法筛选高产胞外多糖的乳酸菌,并采用MTT方法(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT)检测菌株及其胞外多糖对小鼠脾淋巴细胞增殖效果。结果显示,从四川泡菜中分离得到1株植物乳杆菌WHH589产胞外多糖能力最强,产量可达0.58 g/L。MTT实验结果显示,植物乳杆菌WHH589促进小鼠脾淋巴细胞增殖在79.79%~127.55%,其胞外多糖促进小鼠脾淋巴细胞增殖在80.15%~164.42%,具有良好的免疫活性。同时,植物乳杆菌WHH589表现出优良的生长特性以及耐酸、耐胆盐和黏附能力,且发酵乳质构坚实,拉丝明显,长度为25.57 cm,感官和风味良好,活菌数可达4.97×108 CFU/g。说明植物乳杆菌WHH589是1株发酵和益生功能兼具的乳酸菌,具有巨大的开发利用价值。

关键词： 植物乳杆菌WHH589; 胞外多糖; 免疫活性; 酸和胆盐耐受性; 黏附性; 发酵乳

本文引用格式

孙盛 , 陈作国 , 俞赟霞 , 曲冬梅 , 余腾斐 , 李言郡 , 陈苏 . 产胞外多糖植物乳杆菌WHH589的免疫活性及其在发酵乳中的应用[J]. 食品与发酵工业, 2021 , 47(13) : 43 -50 . DOI: 10.13995/j.cnki.11-1802/ts.026564

Abstract

Lactic acid bacteria (LAB) were isolated from Chinese traditional fermented foods to screen the specific strains with high exopolysaccharide yields. The basic characteristics, the immunomodulatory ability and the application in fermented milk were further explored of these LAB. Colony wire drawing test as well as sulfuric acid-phenol method had been utilized to screen the LAB with high exopolysaccharide production, and the effect of LAB on proliferation of mouse spleen lymphocytes were also determined through MTT assay. The results showed that Lactobacillus plantarum WHH589, which separated from Sichuan pickles, exhibited the strongest ability to produce exopolysaccharide. The yield reached 0.58 g/L. The results of MTT assay suggested that L. plantarum WHH589 and exopolysaccharide could significantly promote the proliferation of mouse spleen up to 79.79%-127.55% and 80.15%-164.42%, respectively. which lead to beneficial immunomodulatory effects. In the meanwhile, L. plantarum WHH589 exhibited good growth characteristics, acid, bile salt resistance and intestinal adhesion ability. Moreover, the yogurt fermented by L. plantarum WHH589 possessed a solid texture and obvious drawing with the length reached 25.57 cm. The sensory and flavor were good. The viable count reached 4.97×108 CFU/g. In summary, L. plantarum WHH589 showed great development and utilization value because of fermentation and probiotic properties.

Key words： Lactobacillus plantarum WHH589; extracellular polysaccharides; immunomodulatory; acidic resistance and bile salt tolerance; adherence ability; fermented milk

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