以蜡质玉米淀粉为原料,通过普鲁兰酶脱支结合乙醇梯度溶解法制备平均聚合度(degree of polymerization,DP)分别为14.53、16.20和25.25不同链长的直链糊精,采用共沉淀法与维生素E进行络合,制备直链糊精-维生素E复合物。结果显示,随着直链糊精链长增加,其对维生素E负载率由45.97 μg/mg提高至62.71 μg/mg,包埋率从39.07%增加至59.57%。傅里叶红外变换光谱和X-射线衍射仪证明,不同链长直链糊精与维生素E主要通过氢键形成复合物。热重和光稳定性分析证实,复合物的形成提高了维生素E耐热稳定性以及耐光稳定性,经过48 h紫外灯照射,长链直链糊精对维生素E保留率高达89.19%,而游离维生素E保留率仅为59.52%。结果表明,直链糊精链长增加,其与维生素E复合物耐热、耐光稳定性更强,对维生素E的保护效果更佳。研究为不同链长直链糊精在疏水性客体分子包埋领域的应用提供了重要理论依据。
Three fractions of linear dextrin (LD) with average degree of polymerization (DP) of 14.53, 16.20, and 25.25 were obtained by hydrolyzing waxy maize starch with pullulanase and fractionating using the gradient ethanol dissolution method. Subsequently, LD-VE complexes were prepared by the co-precipitation method. The results showed that the payload capability of LD to vitamin E increased from 45.97 μg/mg to 62.71 μg/mg, and the inclusion rate increased from 39.07% to 59.57% with the increase of chain length. Fourier infrared transform spectrum and X-ray diffraction indicated that hydrogen bonding was the main driving force for complex formation. Thermogravimetric and photochemical stability analysis confirmed that the formation of complex improved the thermal and photostability of vitamin E. After 48 h of UV-irradiation, the retention rate of long-chain LD to vitamin E was up to 89.19%, while free vitamin E was only 59.52%. These results suggested that longer LD-VE inclusion compound showed stronger thermal stability and better protective effect on vitamin E and could guide the encapsulation of macromolecular guests by LD with different DP.
[1] CHUNG H J,LIU Q,LEE L,et al.Relationship between the structure,physicochemical properties and in vitro digestibility of rice starches with different amylose contents[J].Food Hydrocolloids,2011,25(5):968-975.
[2] WANG S X,ZHAN J L,JIN Z Y,et al.Enhanced fluorescence of starch-fluorescence guest complexes enables evaluation of the encapsulation properties of maize starches[J].Food Hydrocolloids,2017,63:286-292.
[3] GELDERS G G,VANDERSTUKKEN T C,GOESAERT H,et al.Amylose-lipid complexation:A new fractionation method[J].Carbohydrate Polymers,2004,56(4):447-458.
[4] OBIRO W C,RAY S S,EMMAMBUX M N.V-amylose structural characteristics,methods of preparation,significance,and potential applications[J].Food Reviews International,2012,28(4):412-438.
[5] HU X T,LIU C M,JIN Z Y,et al.Fractionation of starch hydrolysate into dextrin fractions with low dispersity by gradient alcohol precipitation[J].Separation and Purification Technology,2015,151:201-210.
[6] XU J,ZHAO W X,NING Y W,et al.Can helical spring dextrin be composed of higher eight glucose units per turn[J].Journal of Molecular Structure,2013,1 036:303-309.
[7] 冯涛, 曾小兰,王珂,等.短链葡聚糖-姜黄素纳米乳液的制备及结构表征[J].农业工程学报,2019,35(1):303-309.
FENG T,ZENG X L,WANG K,et al.Preparation and structure characterization of short glucan chain-curcumin nano-emulsion[J].Transactions of the Chinese Society of Agricultural Engineering,2019,35(1):311-317.
[8] WANG P P,QIN X S,YANG Q Y,et al.Comparative structural characterization of spiral dextrin inclusion complexes with vitamin E or soy isoflavone[J].Journal of Agricultural and Food Chemistry,2017,65(39):8 744-8 753.
[9] 宋晓燕, 杨天奎.天然维生素E的功能及应用[J].中国油脂,2000,25(6):45-47.
SONG X Y,YANG T K.Function and application of natural vitamin E[J].China Oils and Fats,2000,25(6):45-47.
[10] HATEGEKIMANA J.对维生素E运输体系的设计及其稳定性和生物利用率的研究[D].无锡:江南大学,2016.
HATEGEKIMANA J.Designing delivery systems of vitamin E to enhance its stability and bioaccessibility[D].Wuxi:Jiangnan University,2016.
[11] 龙吉云. 高含量天然维生素E油微胶囊化研究[D].南昌:南昌大学,2011.
LONG J Y.Study on microencapsulation of high content natural Vitamin E[D].Nanchang:Nanchang University,2011.
[12] CHANG R R,XIONG L,LI M,et al.Fractionation of debranched starch with different molecular weights via edible alcohol precipitation[J].Food Hydrocolloids,2018,83:430-437.
[13] HU X T,HUANG Z H,ZENG Z L,et al.Improving resistance of crystallized starch by narrowing molecular weight distribution[J].Food Hydrocolloids,2020,103:105 641.
[14] 王萍萍. 基于疏水性生物活性因子新型糊精超分子体系的构筑及其性质研究[D].广州:华南理工大学,2018.
WANG P P.Study on preparation and characterization of a novel dextrin supermolecule system based on hydrophobic bioactive materials[D].Guangzhou:South China University of Technology,2018.
[15] MA R R,TIAN Y Q,CHEN L,et al.Effects of cooling rate on retrograded nucleation of different rice starch-aromatic molecule complexes[J].Food Chemistry,2019,294:179-186.
[16] CHANG R R,TIAN Y Q,LU H,et al.Effects of fractionation and heat-moisture treatment on structural changes and digestibility of debranched waxy maize starch[J].Food Hydrocolloids,2020,101:105 488.
[17] LU H,XIONG L,LI M,et al.Separation and characterization of linear glucans debranched from normal corn,potato and sweet potato starches[J].Food Hydrocolloids,2019,89:196-206.
[18] SUN B H,TIAN Y Q,CHEN L,et al.Linear dextrin as curcumin delivery system:Effect of degree of polymerization on the functional stability of curcumin[J].Food Hydrocolloids,2018,77:911-920.
[19] CELEBIOGLU A,UYAR T.Antioxidant vitamin E/cyclodextrin inclusion complex electrospun nanofibers:Enhanced water-solubility,prolonged shelf-life and photostability of vitamin E[J].Journal of Agricultural and Food Chemistry,2017,65(26):5 404-5 412.
[20] WANG P P,LUO Z G,PENG X C.Encapsulation of vitamin E and soy isoflavone using spiral dextrin:Comparative structural characterization,release kinetics,and antioxidant capacity during simulated gastrointestinal tract[J].Journal of Agricultural and Food Chemistry,2018,66(40):10 598-10 607.
[21] 马云标, 朱科学,周惠明.维生素E微胶囊的理化性质表征[J].中国油脂,2010,35(1):55-59.
MA Y B,ZHU K X,ZHOU H M.Physical and chemical characterization of vitamin E microcapsules[J].China Oils and Fats,2010,35(1):55-59.
