食品与发酵工业 >

2021 , Vol. 47 >Issue 13: 274 - 279

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027073

分析与检测

异硫氰酸苯酯柱前衍生高效液相色谱法测定薏苡仁中17种氨基酸的含量

  • 伍清芳 ,
  • 王敏 ,
  • 陈鸿平 ,
  • 陈林 ,
  • 胡媛 ,
  • 刘友平
展开
  • (成都中医药大学 药学院,四川 成都,611137)
硕士研究生(刘友平研究员为通讯作者,E-mail:liu_youping@163.com)

收稿日期: 2021-02-23

  修回日期: 2021-03-15

  网络出版日期: 2021-08-02

基金资助

成都中医药大学西南特色中药资源重点实验室开放研究基金资助项目(2020XSGG003);2018年度“杏林学者”学科人才科研提升计划研究专项(YYZX20180023)

收起

Determination of 17 amino acids in Coix lacryma-jobi seed by HPLC with pre-column derivatization of PITC

  • WU Qingfang ,
  • WANG Min ,
  • CHEN Hongping ,
  • CHEN Lin ,
  • HU Yuan ,
  • LIU Youping
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  • (Pharmacy College,Chengdu University of TCM,Chengdu 611137,China)

Received date: 2021-02-23

  Revised date: 2021-03-15

  Online published: 2021-08-02

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摘要

为研究薏苡仁中氨基酸的种类及含量,采用柱前衍生化结合HPLC测定,以异硫氰酸苯酯(phenyl isothiocyanate,PITC)为衍生剂,色谱柱采用SVEA C18(250 mm×4.6 mm,5 μm),流动相A为乙腈-水(80∶20,体积比),流动相B为乙腈-0.1 mol/L醋酸钠溶液(94∶6,体积比),流速0.8 mL/min,检测波长254 nm。结果显示,该方法下17种氨基酸色谱峰分离效果良好,且均具有良好的线性关系(R2>0.999),薏苡仁中氨基酸平均加样回收率为97%~101%,相对标准偏差为0.89%~4.86%。薏苡仁中共检测到17种氨基酸,其中7种为人体必需氨基酸。以氨基酸总含量为指标,贵州省兴仁县的薏苡仁质量最优。该方法具有成本低、操作简单等优点,可用于薏苡仁中氨基酸的含量测定。

关键词： 薏苡仁; 氨基酸; 高效液相色谱法; 柱前衍生; 主成分分析

本文引用格式

伍清芳 , 王敏 , 陈鸿平 , 陈林 , 胡媛 , 刘友平 . 异硫氰酸苯酯柱前衍生高效液相色谱法测定薏苡仁中17种氨基酸的含量[J]. 食品与发酵工业, 2021 , 47(13) : 274 -279 . DOI: 10.13995/j.cnki.11-1802/ts.027073

Abstract

To investigate the varieties and contents of hydrolyzed amino acids in Coix lacryma-jobi seed, the pre-column derivatization HPLC method was explored. The following conditions were used which including phenyl isothiocyanate (PITC) as derivatization agent with the SVEA C18 column (250 mm×4.6 mm,5 μm), and the mobile phase A was acetonitrile-water (80∶20, V/V); the mobile phase B was acetonitrile-0.1 mol/L sodium acetate solution (94∶6, V/V), the flow rate was 0.8mL/min, and the detection wavelength was 254 nm. The results showed that 17 kinds of amino acids were separated by this method with good linear relationship (R2 > 0.999). The average recovery of Coix lacryma-jobi seed samples was 96.62%-102.62%, RSD was 0.87%-4.86%. A total of 17 amino acids were detected in Coix lacryma-jobi seed, including 8 essential amino acids. Taking the total amino acid content as the index, the quality of Coix lacryma-jobi seed from Xingren County of Guizhou Province was the best. The method with the advantages of low cost and simple operation could be used in the determination of amino acids in Coix lacryma-jobi seed.

Key words： Coix Lacryma-Jobi seed; amino acid; high performance liquid chromatography; pre-column derivatization; principal component analysis

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