为探究魏斯氏菌10d-17的代谢途径,初步了解其基因功能,该研究基于转录组测序技术对魏斯氏菌10d-17进行转录组测序和生物信息学相关分析。试验结果表明,共获得10.41 GB的数据,对其进行过滤去冗余后得到Unigene 6 947条,其中Q20(Phred 数值大于20碱基占总体碱基的百分比)均在98.36%,高质量reads数为983万~1 359万条;共得到1 018个显著差异表达基因,其中实验组中有509个基因表达上调,509个基因表达下调;差异表达基因中772个基因获得GO(Gene Ontology)功能注释信息,主要富集于RNA代谢过程和药物结合;KEGG(Kyoto Encyclopedia of Genes and Genomes)代谢通路注释发现,差异表达基因主要富集在生物化学代谢、次生代谢物的生物合成等相关代谢通路,其中富集于糖酵解和丙酮酸代谢通路的显著差异表达基因有13个;该研究为探究魏斯氏菌10d-17的代谢途径,初步了解其基因功能奠定了基础。
In order to explore the metabolic pathways of Weissella 10d-17 and preliminarily understand its gene functions, transcriptogram sequencing and bioinformatics correlation analysis of 10d-17 bacteria based on RNA-Seq technology were conducted. The results showed that 10.41 GB of data was obtained, and 6 947 Unigene lines were obtained after filtering out the redundancy. Among them, the number of Q20 (Phred value greater than the percentage of 20 bases in the total base) was 98.36%, and the number of high-quality reads was between 9.83 million and 13.59 million. A total of 1 018 significantly differentially expressed genes (DEGs) were obtained, among which 509 genes were up-regulated and 509 were down-regulated in the experimental group. Gene Ontology (GO) functional annotation information was obtained for 772 genes of DEGs, which were mainly concentrated in RNA metabolism process and drug binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway annotation found that DEGs were mainly concentrated in biochemical metabolism, biosynthesis of secondary metabolites and other related metabolic pathways, among which 13 genes were significantly differentially expressed in glycolysis and pyruvate metabolic pathways.
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