食品与发酵工业 >

2021 , Vol. 47 >Issue 14: 123 - 129

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025828

生产与科研应用

耐高温菌株大曲高温放线菌CICC 10681培养条件的响应面优化

  • 刘红强 ,
  • 于学健 ,
  • 翟磊 ,
  • 白飞荣 ,
  • 姚粟
展开
  • (中国食品发酵工业研究院有限公司,中国工业微生物菌种保藏管理中心,北京, 100015)
硕士研究生(姚粟教授为通讯作者,E-mail:milly@china-cicc.org)

收稿日期: 2020-10-12

  修回日期: 2020-11-04

  网络出版日期: 2021-08-20

基金资助

国家重点研发计划资助(2016YFD0400502)

收起

Response surface optimization of the culture conditions of thermotolerant Thermoactinomyces daqus CICC 10681

  • LIU Hongqiang ,
  • YU Xuejian ,
  • ZHAI Lei ,
  • BAI Feirong ,
  • YAO Su
Expand
  • (China National Research Institute of Food and Fermentation Industries Co.Ltd., China Center of Industrial Culture Collection, Beijing 100015, China)

Received date: 2020-10-12

  Revised date: 2020-11-04

  Online published: 2021-08-20

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摘要

为了获得耐高温菌株大曲高温放线菌(Thermoactinomyces daqus, T.daqus) CICC 10681的最佳培养条件,以生物量(OD620nm)为响应值,通过单因素试验、Plackett-Burman、响应面试验对菌株CICC 10681的生长条件进行优化。研究结果表明,T.daqus CICC 10681的最佳培养条件为可溶性淀粉4.5 g/L、大豆蛋白胨8.4 g/L、硫酸镁5 g/L、53 ℃、接种量2%、装液量60 mL/250mL、转速180 r/min,在此条件下进行验证试验,菌株OD620nm达到1.98±0.029,较优化前提高了6.2倍,因此运用响应面试验预测的该模型有一定的实践指导意义,为后续菌株在高温大曲堆积发酵生产中的应用提供理论基础。

关键词： 大曲高温放线菌; 嗜高温; 培养条件; 响应面优化; 高温酶

本文引用格式

刘红强 , 于学健 , 翟磊 , 白飞荣 , 姚粟 . 耐高温菌株大曲高温放线菌CICC 10681培养条件的响应面优化[J]. 食品与发酵工业, 2021 , 47(14) : 123 -129 . DOI: 10.13995/j.cnki.11-1802/ts.025828

Abstract

To obtain the optimal culture conditions of Thermoactinomyces daqus CICC 10681, single-factor test, Plackett-Burman and response surface test were conducted using biomass OD620nm as the response value. The results showed that the optimal culture parameters of T. daqus CICC 10681 were: soluble starch 4.5 g/L, soy peptone 8.4 g/L, MgSO4 5 g/L, 53 ℃, inoculation volume 2%, loaded volume 60 mL/250mL and rotate speed at 180 r/min. Under this condition, the OD620nm value was up to 1.98±0.029, which was 6.2-fold higher than the value before optimization. Therefore, the model has certain practical guidance significance, and provides a theoretical basis for the application of subsequent strains in Daqu accumulation fermentation.

Key words： Thermoactinomyces daqus; thermostable; culture conditions; response surface optimization; thermostable enzymes

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