食品与发酵工业 >

2021 , Vol. 47 >Issue 15: 213 - 219

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027054

生产与科研应用

魔芋葡甘聚糖-大豆分离蛋白-辣椒红色素复合有色凝胶的制备及其性能研究

  • 朱坤 ,
  • 刘缘勤 ,
  • 范盛玉 ,
  • 段雅洁 ,
  • 陈厚荣 ,
  • 张甫生
展开
  • (西南大学 食品科学学院,重庆,400715)
硕士研究生(张甫生副教授为通讯作者,E-mail:zfsswu@163.com)

收稿日期: 2021-02-16

  修回日期: 2021-03-22

  网络出版日期: 2021-08-23

基金资助

重庆市自然科学基金项目(cstc2018jcyjAX0002)

收起

Preparation and characteristics of konjac glucomannan-soybean protein isolate-capsanthin coloring composite gel

  • ZHU Kun ,
  • LIU Yuanqin ,
  • FAN Shengyu ,
  • DUAN Yajie ,
  • CHEN Hourong ,
  • ZHANG Fusheng
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  • (College of Food Science,Southwest University,Chongqing 400715,China)

Received date: 2021-02-16

  Revised date: 2021-03-22

  Online published: 2021-08-23

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摘要

为解决凝胶素食色泽单一、难赋色等问题,拓展凝胶素食在食品领域的应用,该研究以魔芋葡甘聚糖(konjac glucomannan,KGM)、大豆分离蛋白(soybean protein isolate,SPI)和辣椒红色素(capsanthin,CAP)为研究对象,以KGM、SPI、氢氧化钙质量分数为单因素,以色素保留率和凝胶强度为响应值,通过响应面试验得到复合有色凝胶的最佳制备工艺条件。同时利用质构、傅里叶转换红外光谱和扫描电子显微镜对优化制备前后复合有色凝胶的质构和微观结构进行比较分析。结果表明,当 KGM、SPI和氢氧化钙的质量分数分别为6.5%、0.2%和0.15%时,复合有色凝胶性能最佳,在此条件下复合有色凝胶的色素保留率可达到84.26%,凝胶强度达到376.65 g,与预测值相近。同时优化制备后复合有色凝胶的凝胶强度、硬度、咀嚼性显著增大,质构性能提高。电镜结果表明优化制备后复合有色凝胶空间网络连续均匀分布,结构更稳定,具有更好的质构性能。

关键词： 魔芋葡甘聚糖; 大豆分离蛋白; 辣椒红色素; 复合有色凝胶; 微观结构

本文引用格式

朱坤 , 刘缘勤 , 范盛玉 , 段雅洁 , 陈厚荣 , 张甫生 . 魔芋葡甘聚糖-大豆分离蛋白-辣椒红色素复合有色凝胶的制备及其性能研究[J]. 食品与发酵工业, 2021 , 47(15) : 213 -219 . DOI: 10.13995/j.cnki.11-1802/ts.027054

Abstract

To improve the status of the single color and expand the development of konjac gel vegetarian food, this study explored the formulation of coloring composite gel mixed by konjac glucomannan (KGM), soybean protein isolate (SPI) and capsanthin(CAP). The preparation conditions of coloring composite gel were optimized by using KGM concentration, SPI concentration and Ca(OH)2 concentration as the independent variables, while the pigment retention rate and gel strength as the response values. Moreover, the texture properties and microstructure of the composite gel were characterized by TA. XT plus texture, Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). Results showed that the optimum conditions were determined as follows, the concentration of KGM, SPI and Ca(OH)2 were 6.5%, 0.2% and 0.15% respectively. Under these conditions, the pigment retention rate and the gel strength could go up to 84.26% and 376.65 g which close to the predicted value. At the same time the gel strength, hardness and chewiness of the optimization were significantly increased, and its microstructure of the composite gel observed by SEM was uniformly distributed continuously and more stable, both leading to better texture properties compared with control groups.

Key words： konjac glucomannan; soybean protein isolate; capsanthin; coloring composite gel; microstructure

参考文献

[1] RUBY M B.Vegetarianism.A blossoming field of study[J].Appetite,2012,58(1):141-150.
[2] ROSENFELD D L.The psychology of vegetarianism:Recent advances and future directions[J].Appetite,2018,131:125-138.
[3] 吴元浩, 徐婧婷,刘欣然,等.植物基仿肉原料的应用与加工现状[J].食品安全质量检测学报,2020,11(17):5 955-5 963.
WU Y H,XU J T,LIU X R,et al.Application and processing status of plant-based meat analogue ingredients[J].Journal of Food Safety and Quality,2020,11(17):5 955-5 963.
[4] MORENO H M,HERRANZ B,MATEOS M P,et al.New alternatives in seafood restructured products[J].Critical Reviews in Food Science and Nutrition,2016,56(2):237-248.
[5] SOLO-DE-ZAKDÍVAR B S,TOVAR C A,BORDERÍAS A J,et al.Effect of deacetylation on the glucomannan gelation process for making restructured seafood products[J].Food Hydrocolloids,2014,35:59-68.
[6] 李崇高, 黄建初,黄泳梅.魔芋多糖仿生食品工艺的研究[J].西南大学学报(自然科学版),2008,30(1):119-125.
LI C G,HUANG J C,HUANG Y M.Studies on technology of konjac polysaccharide simulated food[J].Journal of Southwest University(Natural Science),2008,30(1):119-125.
[7] 汪师帅, 杨金初.辣椒红色素对魔芋葡甘聚糖凝胶性质的影响[J].食品研究与开发,2018,39(17):21-25.
WANG S S,YANG J C.Effect of capsanthin on properties of konjac glucomannan gels[J].Food Research and Development,2018,39(17):21-25.
[8] HENG L,VAN KONINGSVELD G.A,GRUPPEN H,et al.Protein-flavour interactions in relation to development of novel protein foods[J].Trends in Food Science & Technology,2004,15(3):217-224.
[9] ZHENG L,WANG Z J,KONG Y,et al.Different commercial soy protein isolates and the characteristics of Chiba tofu[J].Food Hydrocolloids,2020,110:106 115.
[10] ZHAO C B,CHU Z J,MIAO Z C,et al.Ultrasound heat treatment effects on structure and acid-induced cold set gel properties of soybean protein isolate[J].Food Bioscience,2021,39:100 827.
[11] 郭顺堂, 徐婧婷,刘欣然,等.我国植物蛋白资源高效利用途径与技术创新[J].食品科学技术学报,2019,37(6):8-15.
GUO S T,XU J J,LIU X R,et al.Efficient utilization and technological innovation of plant-based protein resources in China[J].Journal of Food Science and Technology,2019,37(6):8-15.
[12] TAPAL A,TIKU P K.Complexation of curcumin with soy protein isolate and its implications on solubility and stability of curcumin[J].Food Chemistry,2012,130(4):960-965.
[13] CHEN Z Q,WANG C,GAO X D,et al.Interaction characterization of preheated soy protein isolate with cyanidin-3-O-glucoside and their effects on the stability of black soybean seed coat anthocyanins extracts[J].Food Chemistry,2019,271:266-273.
[14] CHEN G,WANG S T,FENG B,et al.Interaction between soybean protein and tea polyphenols under high pressure[J].Food Chemistry,2019,277:632-638.
[15] 黎智华, 王恬.辣椒红素的生物利用度、生理功能及机制研究进展[J].食品科学,2020,41(11):259-266.
LI Z H,WANG T.Progress in understanding the bioavailability,physiological functions and mechanisms of action of capsanthin[J].Food Science,2020,41(11):259-266.
[16] 陈菁, 黄明.影响辣椒红色素稳定性的因素研究[J].食品工业科技,2014,35(4):287-290.
CHEN J,HUANG M.Study on factors affecting the stability of Capsicum red pigment[J].Science and Technology of Food Industry,2014,35(4):287-290.
[17] 马嫄, 付晓,孟晓,等.辣椒红色素微胶囊化的工艺研究[J].食品科技,2010,35(4):228-231.
MA Y,FU X,MENG X,et al.Study on the capsanthin microencapsulation technology[J].Food Science and Technology,2010,35(4):228-231.
[18] 唐永富, 査勇,李积华,等.微胶囊化辣椒红色素工艺研究[J].食品科学,2009,30(20):207-210.
TANG Y F,ZHA Y,LI J H,et al.Optimization of microencapsulation of paprika oleoresin[J].Food Science,2009,30(20):207-210.
[19] 徐晓萍, 张亚宁,樊巧,等.响应面法优化制备魔芋葡甘聚糖-大豆分离蛋白混合凝胶[J].食品科学,2016,37(4):44-50.
XU X P,ZHANG Y N,FAN Q,et al.Optimization of the formulation of konjac glucomannan-soybean protein isolate mixed gel by response surface methodology[J].Food Science,2016,37(4):44-50.
[20] 陈洁, 林华庆,余楚钦,等.辣椒红色素色价与含量的关系及提高稳定性的方法[J].安徽农业科学,2015,43(10):16-18.
CHEN J,LIN H Q,YU C Q,et al.Relationship of color value of capsanthin and content and method to improve stability[J].Journal of Anhui Agricultural Sciences,2015,43(10):16-18.
[21] ZHANG Y T,TAN C,ABBAS S,et al.The effect of soy protein structural modification on emulsion properties and oxidative stability of fish oil microcapsules[J].Colloids and Surfaces B:Biointerfaces,2014,120:63-70.
[22] NIETO T V N,WANG Y X,OZIMEK L,et al.Improved thermal gelation of oat protein with the formation of controlled phase-separated networks using dextrin and carrageenan polysaccharides[J].Food Research International,2016,82:95-103.
[23] 杨莉莉, 熊善柏,孙建清.制备条件对鱼肉-魔芋胶复合凝胶品质的影响[J].食品科学,2010,31(3):55-59.
YANG L L,XIONG S B,SUN J Q.Effect of processing technology on properties of surimi-konjac compound gel[J].Food Science,2010,31(3):55-59.
[24] 龚加顺, 幸治梅,彭春秀,等.高浓度魔芋葡苷聚糖与大豆分离蛋白混合凝胶质地特性研究[J].食品科学,2005,26(7):35-39.
GONG J S,XING Z M,PENG C X,et al.Study on the textural properties of the gel formed with mixtures of konjac glucomannan and soybean isolate protein at high concentration[J].Food Science,2005,26(7):35-39.
[25] 田学智, 龙昌洲,王子宇,等.高静压处理对魔芋葡甘聚糖/大豆分离蛋白复合溶胶特性的影响[J].食品与发酵工业,2017,43(11):153-158.
TIAN X Z,LONG C Z,WANG Z Y,et al.Effect of high hydrostatic pressure treatment on the functional properties of konjac glucomannan/soybean protein composite sol[J].Food and Fermentation Industries,2017,43(11):153-158.
[26] LIU J J,ZHU K K,YE T,et al.Influence of konjac glucomannan on gelling properties and water state in egg white protein gel[J].Food Research International,2013,51(2):437-443.
[27] LUO X G,HE P,LIN X Y.The mechanism of sodium hydroxide solution promoting the gelation of konjac glucomannan (KGM)[J].Food Hydrocolloids,2013,30(1):92-99.
[28] DU X Z,LI J,CHEN J,et al.Effect of degree of deacetylation on physicochemical and gelation properties of konjac glucomannan[J].Food Research International,2012,46(1):270-278.
[29] WILLIAMS M A K,FOSTER T J,MARTIN D R,et al.A molecular description of the gelation mechanism of konjac mannan[J].Biomacromolecules,2000,1(3):440-450.
[30] WANG L,XIAO M,DAI S H,et al.Interactions between carboxymethyl konjac glucomannan and soy protein isolate in blended films[J].Carbohydrate Polymers,2014,101:136-145.
[31] TANG R P,DU Y M,ZHENG H,et al.Preparation and characterization of soy protein isolate-carboxymethylated konjac glucomannan blend films[J].Journal of Applied Polymer Science,2003,88(5):1 095-1 099.
[32] CHEN Z J,WANG S S,SHANG L C,et al.An efficient and simple approach for the controlled preparation of partially degraded konjac glucomannan[J].Food Hydrocolloids,2020,108:106 017.
[33] 朱建华, 邹秀容,刘日斌,等.超声对蔗糖共溶质场中大豆分离蛋白/魔芋胶共混体系流变及凝胶性质的影响[J].中国粮油学报,2019,34(9):46-52.
ZHU J H,ZOU X R,LIU R B,et al.Effects of ultrasonication on the rheological and gel properties of soybean isolated protein/konjac gum blend under sucrose cosolute environment[J].Journal of the Chinese Cereals and Oils Association,2019,34(9):46-52.
[34] CAKIR E,KHAN S A,FOEGEDING E A.The effect of pH on gel structures produced using protein-polysaccharide phase separation and network inversion[J].International Dairy Journal,2012,27(1-2):99-102.
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