食品与发酵工业 >

2021 , Vol. 47 >Issue 16: 90 - 96

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026807

研究报告

外源基因表达对解脂亚罗酵母糖醇代谢影响的初步研究

  • 周芸琪 ,
  • 李红娇 ,
  • 李克文 ,
  • 郭蓓 ,
  • 裴疆森
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  • 1(中国食品发酵工业研究院有限公司,北京,100015)
    2(保龄宝生物股份有限公司,山东 禹城,251200)
    3(北京农学院 生物与资源环境学院,北京,102206)
硕士研究生(裴疆森教授级高级工程师,E-mail:pei21cn@163.com)

收稿日期: 2021-01-20

  修回日期: 2021-02-28

  网络出版日期: 2021-09-10

基金资助

山东省重大科技创新工程项目(2019JZZY011005)

收起

Effect of exogenous gene expression on sugar alcohol metabolism of Yarrowia lipolytica

  • ZHOU Yunqi ,
  • LI Hongjiao ,
  • LI Kewen ,
  • GUO Bei ,
  • PEI Jiangsen
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  • 1(China National Research Institute of Food and Fermentation Industries, Beijing 100015, China)
    2(Bao Ling Bao Biology Company Limited, Yucheng 251200, China)
    3(College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing 102206, China)

Received date: 2021-01-20

  Revised date: 2021-02-28

  Online published: 2021-09-10

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摘要

解脂亚罗酵母是重要的真核表达宿主,也是当前国内赤藓糖醇发酵的主流菌种。该文将克隆异源酵母中提取的一个推定具有糖磷酸化功能的基因,通过构建表达载体并转入解脂亚罗酵母中进行表达,考察重组菌株与赤藓糖醇合成相关底物利用和转化的影响。结果显示,解脂亚罗酵母重组菌株与亲本菌株相比在摇瓶发酵实验中对赤藓糖醇和核糖的同化存在差异性,重组菌株在基质中含有葡萄糖和赤藓糖醇或核糖时,同化能力至少为亲本菌株的2倍,该功能基因为具有赤藓糖醇合成调控能力的新基因;将重组菌株用于赤藓糖醇母液的处理试验表明,重组菌株与对照菌株相比可同化母液中的多种糖醇用于细胞的生长,能改变结晶母液组分降低母液黏度和麦芽糖浓度,为工业废液的综合利用提供了新的解决方案。

关键词： 解脂亚罗酵母; 异源表达; 赤藓糖醇; 单拷贝整合; 母液

本文引用格式

周芸琪 , 李红娇 , 李克文 , 郭蓓 , 裴疆森 . 外源基因表达对解脂亚罗酵母糖醇代谢影响的初步研究[J]. 食品与发酵工业, 2021 , 47(16) : 90 -96 . DOI: 10.13995/j.cnki.11-1802/ts.026807

Abstract

Yarrowia lipolytica is a widely used eukaryotic expression host and a major strain for erythritol fermentation in China. In this research, a putative gene with sugar phosphorylation function cloned from Zygoascus hellenicus was constructed into an expression vector and transformed into Y. lipolytica. The effect of the recombinant strain on substrate utilization and conversion related to erythritol synthesis was investigated. The results showed that in the shake flask fermentation, different assimilation of erythritol and ribose was observed in the recombinant Y. lipolytica compared with the original strain. With glucose and erythritol/ribose in the medium, the assimilation ability of the recombinant strain was at least twice that of the original strain. This functional gene was a new gene with the ability to regulate erythritol synthesis. The treatment experiment of erythritol waste liquor showed that the recombinant strain could assimilate various sugar alcohols for cell growth compared with the control strain, and change the composition of the crystallization waste liquor to reduce the viscosity and maltose concentration, providing a new solution for the comprehensive utilization of industrial waste liquor.

Key words： Yarrowia lipolytica; heterologous expression; erythritol; single copy integration; waste liquor

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