食品与发酵工业 >

2021 , Vol. 47 >Issue 16: 253 - 257

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027330

分析与检测

KI-罗丹明6G荧光猝灭法测定火腿中的亚硝酸盐

  • 庞海霞 ,
  • 强瑾 ,
  • 姜敏 ,
  • 熊海涛 ,
  • 史娟
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  • (陕西理工大学 化学与环境科学学院,陕西省催化基础与应用重点实验室,陕西 汉中,723001)
硕士,讲师(本文通讯作者,E-mail:phx124@126.com)

收稿日期: 2021-03-15

  修回日期: 2021-04-29

  网络出版日期: 2021-09-10

基金资助

陕西省科技厅社会发展科技攻关项目(2018SF-307);陕西省科技厅自然科学研究项目(2019JM-545);陕西理工大学人才启动项目(SLGRC07)

收起

Determination of nitrite in ham sausage by KI-rhodamine 6G fluorescence quenching method

  • PANG Haixia ,
  • QIANG Jin ,
  • JIANG Min ,
  • XIONG Haitao ,
  • SHI Juan
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  • (Shaanxi Key Laboratory of Catalysis, School of Chemical and Environmental Sciences, Shaanxi University of Technology, Hanzhong 723001, China)

Received date: 2021-03-15

  Revised date: 2021-04-29

  Online published: 2021-09-10

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摘要

酸性条件下,NO-2与过量的KI反应生成I-3阴离子,I-3可与罗丹明6G形成离子缔合物,使得罗丹明6G发生荧光猝灭,且体系的荧光猝灭值ΔF与NO-2的质量浓度呈良好的线性关系,该文据此原理建立了一种测定NO-2的新方法。通过控制变量法确定了该方法的最佳测定条件为:0.1 mol/L H2SO4溶液2.0 mL、0.1 mol/L KI溶液2.0 mL、1.0 g/L吐温-80溶液0.5 mL、5×10-5 mol/L罗丹明6G溶液2.0 mL,荧光激发波长477 nm、发射波长552 nm,在此条件下测定水溶液中NO-2的线性范围为30~240 μg/L,回归方程为ΔF=1.944 4ρ-25.178,相关系数r=0.997 5,检出限为20.3 μg/L,相对标准偏差为2.7%(ρ=160 μg/L,n=11),回收率为95.5%~105.3%。将该方法用于火腿肠中亚硝酸盐含量的测定,效果良好。

关键词： KI-罗丹明6G; 荧光猝灭; 亚硝酸盐; 测定; 火腿

本文引用格式

庞海霞 , 强瑾 , 姜敏 , 熊海涛 , 史娟 . KI-罗丹明6G荧光猝灭法测定火腿中的亚硝酸盐[J]. 食品与发酵工业, 2021 , 47(16) : 253 -257 . DOI: 10.13995/j.cnki.11-1802/ts.027330

Abstract

Under acidic conditions, NO-2 reacts with excess KI to form I-3anion. And I-3 forms an ion association with rhodamine 6G which quenched the fluorescence quenching of the complex, and the fluorescence quenching values of the system were F and NO-2. The mass concentration had a good linear relationship, and a new method for determining NO-2 was established accordingly. The optimal measurement conditions of the method were determined by the controlled variable method. These conditions were 0.1 mol/L H2SO4 solution 2.0 mL, 0.1 mol/L KI solution 2.0 mL, 1.0 g/L Tween-80 solution 0.5 mL, 5.0×10-5 mol/L Rhodamine 6G solution 2.0 mL, fluorescence excitation wavelength 477 nm, emission wavelength 552 nm. Under these conditions, the linear range of NO-2 in aqueous solution was 30-240 μg/L and the regression equation was ΔF=1.944 4ρ-25.178. In addition, the coefficient r=0.997 5 and the detection limit was 20.3 μg/L. Moreover, the precision relative standard deviation (RSD) was 2.7%(ρ=160 μg/L, n=11) and the recovery was between 95.5% and 105.3%. This method can be used for the determination of nitrite content in ham sausage with satisfactory results.

Key words： KI-rhodamine 6G; fluorescence quenching; nitrite; determination; ham sausage

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