食品与发酵工业 >

2021 , Vol. 47 >Issue 16: 258 - 262

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027120

分析与检测

气相色谱-正化学源质谱法同时测定焦糖色素中2-甲基咪唑和4-甲基咪唑

  • 乔青青 ,
  • 郝莉花 ,
  • 巩凡 ,
  • 葛静 ,
  • 方裕科
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  • 1(河南省产品质量监督检验院,河南 郑州,450000)
    2(河南省食品安全数据智能重点实验室,河南 郑州,450000)
    3(仲恺农业工程学院,广东 广州,510000)
硕士,高级工程师(郝莉花高级工程师为通讯作者,E-mail:522007712@qq.com)

收稿日期: 2021-02-25

  修回日期: 2021-04-16

  网络出版日期: 2021-09-10

基金资助

河南省重点研发与推广专项(科技攻关)项目(202102310295);河南省市场监督管理局科技计划项目(2020sj22);河南省市场监督管理局科技计划项目(2021sj24)

收起

Determination of 2-methylimidazole and 4-methylimidazole in caramel pigment using GC-PCI MS/MS

  • QIAO Qingqing ,
  • HAO Lihua ,
  • GONG Fan ,
  • GE Jing ,
  • FANG Yuke
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  • 1(Henan Institute of Quality Supervision and Inspection, Zhengzhou 450000, China)
    2(Henan key laboratory of food safety data intelligence, Zhengzhou 450000, China)
    3(Zhongkai University of Agriculture and Engineering, Guangzhou 510000, China)

Received date: 2021-02-25

  Revised date: 2021-04-16

  Online published: 2021-09-10

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摘要

建立了采用气相色谱-正化学源质谱法同时检测焦糖色素中2-甲基咪唑和4-甲基咪唑的方法。样品经碱化处理后,用硅藻土净化,乙酸乙酯洗脱,采用气相色谱-正化学源质谱法测定。结果表明,在0.5~8.0 μg/mL线性良好,2-甲基咪唑R2=0.999 7,4-甲基咪唑R2=0.999 4;2种物质的方法检出限均为20 mg/kg;在20、80、320 mg/kg的3个水平下,2-甲基咪唑的回收率为102.1%~107.9%,相对标准偏差(n=3)为1.33%~5.79%。该方法简单、快速、高效,可用于焦糖色素中2-甲基咪唑和4-甲基咪唑的同时测定。

关键词： 气相色谱-正化学源质谱; 焦糖色素; 2-甲基咪唑; 4-甲基咪唑

本文引用格式

乔青青 , 郝莉花 , 巩凡 , 葛静 , 方裕科 . 气相色谱-正化学源质谱法同时测定焦糖色素中2-甲基咪唑和4-甲基咪唑[J]. 食品与发酵工业, 2021 , 47(16) : 258 -262 . DOI: 10.13995/j.cnki.11-1802/ts.027120

Abstract

Gas chromatography-positive chemical ionization-tandem triple quadruple mass spectrometry (GC-PCI MS/MS) for determination of 2-methylimidazole and 4-methylimidazole residue in caramel pigment was established. The samples were first alkalized and purified by kieselguhr. And then they were eluted with ethyl acetate. The detection was carried out by GC-PCI MS/MS. A good linear relationship was obtained in the concentrations of 0.5-8.0 μg/mL, while the correlation coefficient of 2-methylimidazole was 0.999 7 and 4-methylimidazole was 0.999 4 respectively. The detection limit of this method was 20 mg/kg. The recoveries of three spiked levels (20, 80 and 320 mg/kg) were in the range of 102.1%-107.9% with RSDs (n=3) which between 1.33%-5.79%. This method developed here was simple, rapid, sensitive and accurate to meet the needs of the analysis. And it could be used for the detection of 2-methylimidazole and 4-methylimidazole in caramel pigment.

Key words： gas chromatography-positive chemical ionization-tandem triple quadruple mass spectrometry (GC-PCI MS/MS); caramel pigment; 2-methylimidazole; 4-methylimidazole

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