采用葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导急性结肠炎模型,评价青霉素干预对结肠炎发展的影响,并考察青霉素干预后灌胃植物乳杆菌AR113的效果。选取雄性SPF级C57BL/6 J鼠进行青霉素干预处理3周后进行DSS诱导,随后进行AR113灌胃治疗,测定了AR113处理和抗生素干预处理对小鼠结肠炎症状、炎症水平以及肠道屏障的影响。结果显示,青霉素干预组的疾病活动指数、体重、髓过氧化物酶活性、组织损伤及炎症因子的变化显著低于模型组,即青霉素干预处理能够钝化DSS诱导结肠炎的敏感性。益生菌联合处理组(青霉素干预后灌胃植物乳杆菌AR113)的结肠炎症状也显著优于模型组,但是与青霉素干预组相比无显著差异,青霉素与AR113联合处理并不能提高对DSS结肠炎的治疗效果。进一步分析显示,青霉素干预组以及联合处理对肠道黏液层的恢复效果无显著差异,但是显著低于植物乳杆菌AR113单独灌胃组。即抗生素处理后,灌胃植物乳杆菌AR113并不会干扰肠道上皮细胞黏液层的恢复,但是抗生素会降低黏液层的恢复效果。
The model group of acute colitis was induced by dextran sulfate sodium (DSS). The effect of penicillin and Lactobacillus plantarum AR113 on model group were studied by detect the symptoms of colitis, inflammation level and intestinal barrier. The results showed that the disease activity index (DAI), body weight, myeloperoxidase (MPO) activity, tissue damage and inflammatory in penicillin group were lower than the model group, which suggesting that the sensitivity of DSS-induced colitis was inactivated by penicillin. The combined treatment of penicillin and AR113 group exhibited lower colitis symptom than that of the model group, but no significant different to the penicillin group. In addition, the recovery of intestinal mucus layer showed no significant difference between the penicillin group and the combined treatment group. However, the recovery ability on intestinal mucus layer in AR113 group was better than the penicillin group and combined treatment group. In conclusion the treatment of AR113 gavage affect little on the recovery of intestinal mucus layer after penicillin intervention, while penicillin will reduce the recovery effect of the mucus layer.
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