食品与发酵工业 >

2021 , Vol. 47 >Issue 18: 37 - 45

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027601

研究报告

水分活度对轻腌大黄鱼源蜂房哈夫尼菌碳源利用能力的影响

  • 王晓阳 ,
  • 郭全友 ,
  • 黄海潮 ,
  • 杨絮 ,
  • 张小敏
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  • 1(上海海洋大学 食品学院,上海,201306)
    2(中国水产科学研究院东海水产研究所,上海,200090)
    3(上海理工大学 医疗器械与食品学院,上海,200093)
硕士研究生(郭全友研究员为通讯作者,E-mail:dhsguoqy@163.com)

收稿日期: 2021-04-02

  修回日期: 2021-05-28

  网络出版日期: 2021-10-18

基金资助

国家自然科学基金(31871872);中国水产科学研究院基本科研业务费资助项目(2020TD68);中央级公益性科研院所基本科研业务费专项资金(2018M04)

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Effects of water activity on carbon source utilization ability of Hafnia alvei isolated from lightly salted Larimichthys crocea

  • WANG Xiaoyang ,
  • GUO Quanyou ,
  • HUANG Haichao ,
  • YANG Xu ,
  • ZHANG Xiaomin
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  • 1(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)
    2(East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China)
    3(School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)

Received date: 2021-04-02

  Revised date: 2021-05-28

  Online published: 2021-10-18

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摘要

为探究水分活度(water activity,Aw)对轻腌大黄鱼源蜂房哈弗尼菌碳源代谢的影响,利用Biolog GEN Ⅲ微孔板测定25 ℃、不同Aw(0.92、0.93、0.94、0.95、0.96、0.97、0.98)下该菌碳源利用情况,采用修正的Gompertz模型拟合代谢曲线,获取动力学参数,结合平均颜色变化率及利用面积,探究其不同Aw下碳源利用效果及动力学特征。结果表明,不同Aw(0.92~0.98)下蜂房哈弗尼菌羧酸类(37.3%)和氨基酸类(32.6%)利用率均值较高,Aw为0.98时菌株总体碳源利用能力最强,0.96时次之,剩余组由强到弱依次为0.95、0.92、0.93、0.94、0.97。各Aw下菌株生长利用的优势单一碳源主要有L-岩藻糖、D-岩藻糖、L-鼠李糖、D-葡糖醛酸、乙酰乙酸、乙酸、D-丝氨酸、L-丙氨酸、L-天冬氨酸、N-乙酰-D-葡糖胺、L-组氨酸、D-葡糖-6-磷酸和D-果糖-6-磷酸。随着Aw升高,菌株碳源利用最大比生长速率(μmax)和迟滞期(λ)分别呈现增大和减小的趋势。通过调控底物碳源及环境因子等手段,探究蜂房哈弗尼菌碳源利用和生长动力学,可为靶向抑菌及大黄鱼产品工艺优化提供一定支撑。

关键词： 轻腌大黄鱼; 特定腐败菌; 蜂房哈弗尼菌; 碳源利用; 水分活度; 生长动力学

本文引用格式

王晓阳 , 郭全友 , 黄海潮 , 杨絮 , 张小敏 . 水分活度对轻腌大黄鱼源蜂房哈夫尼菌碳源利用能力的影响[J]. 食品与发酵工业, 2021 , 47(18) : 37 -45 . DOI: 10.13995/j.cnki.11-1802/ts.027601

Abstract

To explore the effect of water activity (Aw) on the carbon metabolism of Hafnia alvei from lightly salted Larimichthys crocea, the carbon source utilization of the strain was measured by Biolog GEN Ⅲ microplate under different Aw (0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98) at 25 ℃. The modified Gompertz model was used to fit the metabolic curve and obtain the kinetic parameters. And combining the average well color development (AWCD) and the utilization area, the utilization effect and dynamic characteristics of carbon source under different Aw were explored. The results showed that for H. alvei, the average utilization rate of carboxylic acids (37.3%) and amino acids (32.6%) was higher under different Aw (0.92-0.98). The overall carbon source utilization capacity was the strongest at Aw of 0.98, followed by 0.96. And the remaining group from strong to weak was 0.95, 0.92, 0.93, 0.94 and 0.97. The dominant single carbon sources for the growth of H. alvei were mainly L-fucose, D-fucose, L-rhamnose, D-glucuronic acid, acetoacetic acid, acetic acid, D-serine, L-alanine, L-aspartic acid, N-acetyl-D-glucosamine, L-histidine, D-glucose-6-PO4 and D-fructose-6-PO4 at Aw of 0.92-0.98. With the increase of Aw, the maximum specific growth rate (μmax) and lag phase duration (λ) of the carbon source utilization of H. alvei showed the trend of increasing and decreasing respectively. Through regulating the carbon source of substrate and environmental factors, the carbon source utilization and growth dynamics of H. alvei from the lightly salted L. crocea were explored, which could provide certain support for targeting bacteriostasis and process optimization of L. crocea.

Key words： lightly salted Larimichthys crocea; specific spoilage organism; Hafnia alvei; carbon source utilization; water activity; growth dynamics

参考文献

[1] 农业农村部渔业渔政管理局. 中国渔业统计年鉴[M].北京:中国农业出版社, 2020:26.
Fisheries Administration of Ministry of Agriculture and Rural Affairs.China Fishery Statistical Yearbook[M].Beijing:China Agriculture Press, 2020:26.
[2] 吴燕燕, 陶文斌, 郝志明, 等.含盐量对腌制大黄鱼鱼肉品质的影响[J].食品与发酵工业, 2019, 45(21):102-109.
WU Y Y, TAO W B, HAO Z M, et al.Effects of different salt concentration on the quality of cultured large yellow croaker (Larimichthys crocea)[J].Food and Fermentation Industries, 2019, 45(21):102-109.
[3] 廖慧琦, 吕春霞, 陆益钡, 等.食盐添加量对养殖大黄鱼鱼糜凝胶特性的影响[J].食品与发酵工业, 2020, 46(20):67-71.
LIAO H Q, LYU C X, LU Y B, et al.Effects of salt concentration on gel properties of cultured large yellow croaker surimi[J].Food and Fermentation Industries, 2020, 46(20):67-71.
[4] GUO Q Y, ZHU Y Q, WANG L M, et al.Shelf life prediction and bacterial flora for the fresh and lightly salted Pseudosciaena crocea stored at different temperatures[J].Emirates Journal of Food and Agriculture, 2018, 30(1):39-48.
[5] WILLIAMS S L, JENSEN R V, KUHN D D, et al.Analyzing the metabolic capabilities of a Vibrio parahaemolyticus strain that causes early mortality syndrome in shrimp[J].Aquaculture, 2017, 476:44-48.
[6] 郭全友, 修艳辉, 王鲁民, 等.鱼源莓实假单胞菌生长动力学与碳源利用分析[J].农业机械学报, 2017, 48(6):319-327.
GUO Q Y, XIU Y H, WANG L M, et al.Growth kinetics and carbon utilization analysis of Pseudomonas fragi from fish[J].Transactions of the Chinese Society for Agricultural Machinery, 2017, 48(6):319-327.
[7] 单珂, 郭全友, 李保国.NaCl和pH对普通变形杆菌和蜂房哈弗尼菌碳源代谢能力的影响[J].食品工业科技, 2019, 40(10):168-173.
SHAN K, GUO Q Y, LI B G.Effects of NaCl and pH on carbon source metabolism ability of Proteus vulgaris and Hafnia alvei[J].Science and Technology of Food Industry, 2019, 40(10):168-173.
[8] 绳金房, 李辉, 马仕洪, 等.浅析水分活度测定在非无菌制剂微生物控制中的应用[J].药物分析杂志, 2018, 38(10):1 837-1 841.
SHENG J F, LI H, MA S H, et al.Application of water activity determination of microbial control in nonsterile pharmaceutical products[J].Chinese Journal of Pharmaceutical Analysis, 2018, 38(10):1 837-1 841.
[9] VERSCHUERE L, FIEVEZ V, VAN VOOREN L, et al.The contribution of individual populations to the biolog pattern of model microbial communities[J].FEMS Microbiology Ecology, 1997, 24(4):353-362.
[10] HOWARD P J A.Analysis of inter-sample distances from BIOLOG plate data in Euclidean and simplex spaces[J].Soil Biology and Biochemistry, 1999, 31(9):1 323-1 330.
[11] MEKONNEN E, KEBEDE A, TAFESSE T, et al.Investigation of carbon substrate utilization patterns of three ureolytic bacteria[J].Biocatalysis and Agricultural Biotechnology, 2019, 22:101 429.
[12] DALGAARD P.Modelling of microbial activity and prediction of shelf-life for packed fresh fish[J].International Journal of Food Microbiology, 1995, 26(3):305-317.
[13] WEBER K P, GROVE J A, GEHDER M, et al.Data transformations in the analysis of community-level substrate utilization data from microplates[J].Journal of Microbiological Methods, 2007, 69(3):461-469.
[14] IVANOVA E P, NEDASHKOVSKAYA O I, ZHUKOVA N V, et al.Shewanella waksmanii sp.nov., isolated from a sipuncula (Phascolosoma japonicum)[J].International Journal of Systematic and Evolutionary Microbiology, 2003, 53(5):1 471-1 477.
[15] 张佩华, 梅子, 傅玉颖, 等.食品级单辛酸甘油酯微乳液的抑菌研究[J].食品工业科技, 2013, 34(13):136-138;142.
ZHANG P H, MEI Z, FU Y Y, et al.Antimicrobial activity evaluation of food grade glycerol mono-octanoate microemulsion[J].Science and Technology of Food Industry, 2013, 34(13):136-138;142.
[16] 郭全友, 董艺伟, 李保国, 等.淡腌青鱼中木糖葡萄球菌碳源代谢能力研究[J].农业机械学报, 2016, 47(10):258-266.
GUO Q Y, DONG Y W, LI B G, et al.Potential of Staphylococcus xylosus isolated from lightly salted Mylopharyngodon piceus to utilize carbon sources[J].Transactions of the Chinese Society for Agricultural Machinery, 2016, 47(10):258-266.
[17] MARINEZ-MOYA P, NIEHAUS K, ALCAíNO J,et al.Proteomic and metabolomic analysis of the carotenogenic yeast Xanthophyllomyces dendrorhous using different carbon sources[J].BMC Genomics, 2015, 16(1):289.
[18] 曹慧, 李洁, 彭少杰, 等.馒头中金黄色葡萄球菌的控制工艺研究[J].食品与发酵科技, 2016, 52(6):52-56;112.
CAO H, LI J, PENG S J, et al.Study on control of Staphylococcus aureus in steamed bread[J].Food and Fermentation Sciences & Technology, 2016, 52(6):52-56;112.
[19] PARK H W, CHEN G Y, HWANG C A, et al.Effect of water activity on inactivation of Listeria monocytogenes using gaseous chlorine dioxide-A kinetic analysis[J].Food Microbiology, 2021, 95(7):103 707.
[20] RADKOV A D, MOE L A.Bacterial synthesis of D-amino acids[J].Applied Microbiology＆Biotechnology, 2014, 98(12):5 363-5 374.
[21] 乐毅全, 王士芬, 朱核光.脱色菌腐败希瓦氏菌的分离及其脱色性能研究[J].环境科学与技术, 2004, 27(3):14-15;37.
LI Y Q, WANG S F, ZHU H G.Isolation of a dye decoloring bacterium and its characteristics[J].Environmental Science & Technology, 2004, 27(3):14-15;37.
[22] 修艳辉, 郭全友, 韩承义,等.鱼源腐败希瓦氏菌碳源利用及动力学分析[J].食品科学, 2018, 39(2):80-87.
XIU Y H, GUO Q Y, HAN C Y,et al.Carbon utilization profile and growth kinetics of Shewanella putrefaciens isolated from spoiling fish[J].Food Science, 2018, 39(2):80-87.
[23] 修艳辉, 郭全友, 姜朝军.pH、水分活度和NaCl对腐败希瓦氏菌生长/非生长界限及生长动力学参数的影响[J].现代食品科技, 2016, 32(6):156-162;199.
XIU Y H, GUO Q Y, JIANG C J.Effect of pH, water activity, and common salt on the growth/no growth boundary and growth kinetic parameters of Shewanella putrefaciens[J].Modern Food Science and Technology, 2016, 32(6):156-162;199.
[24] 朱彦祺, 郭全友, 姜朝军, 等.新鲜大黄鱼优势腐败菌碳源利用的差异性分析[J].食品科学, 2018, 39(16):176-185.
ZHU Y Q, GUO Q Y, JIANG C J, et al.Analysis of the difference in carbon source utilization profiles of the dominant spoilage bacteria in fresh Pseudosciaena crocea stored at different temperatures[J].Food Science, 2018, 39(16):176-185.
[25] 曲同宝, 于淼, 朱悦, 等.松嫩草地豆类草群落土壤细菌碳源利用研究[J].草地学报, 2018, 26(2):356-362.
QU T B, YU M, ZHU Y, et al.Utilization of carbon sources by soil bacteria in different leguminous communities of Songnen Steppe[J].Acta Agrestia Sinica, 2018, 26(2):356-362.
[26] ROOPESH M S, JUMING T, VILLA-ROJAS R,et al.Influence of water activity on thermal resistance of microorganisms in low-moisture foods:A review[J].Comprehensive Reviews in Food Science and Food Safety, 2016, 15(2):353-370.
[27] 马艳, 李婷婷, 崔方超, 等.不同培养条件及群体感应信号分子对蜂房哈夫尼亚菌生物被膜的影响[J].食品科学, 2017, 38(6):42-47.
MA Y, LI T T, CUI F C, et al.Influences of different culture conditions and quorum sensing signaling molecules on the biofilm formation of Hafnia alvei[J].Food Science, 2017, 38(6):42-47.
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