研究4-甲基愈创木酚对酒精性肝损伤小鼠的保护作用。将50只小鼠随机分为5组,分别为空白组、模型组及4-甲基愈创木酚低、中、高3个剂量组,经口灌胃按照分组分别给予等量的生理盐水、50%食用酒精和低、中、高剂量浓度的4-甲基愈创木酚,实验持续 8周,末次灌胃后各组实验动物隔夜禁食12 h;12 h后从眼球采血,测定小鼠血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density liptein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)浓度及谷草转氨酶(aspartate aminotransferase,AST)、谷丙转氨酶(alanine aminotransferase,ALT)的活力;肝脏组织匀浆中总超氧化物歧化酶(superoxide dismutase,SOD)活力、还原型谷胱甘肽(glutathione,GSH)浓度、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力、过氧化氢酶(catalase,CAT)活力、丙二醛(malondialdehyde,MDA)浓度。4-甲基愈创木酚低、中、高剂量组小鼠血清中TC、TG、LDL-C浓度均低于模型组,AST、ALT活力均低于模型组,HDL-C浓度高于模型组;4-甲基愈创木酚低、中、高剂量组小鼠肝组织匀浆中SOD活力、CAT活力、GSH-Px 活力和 GSH 浓度均高于模型组,而MDA浓度均低于模型组(P＜0.05),且与4-甲基愈创木酚浓度有关。4-甲基愈创木酚对酒精性肝损伤具有保护作用且与剂量有关。4-甲基愈创木酚通过降低肝组织的损伤、减少脂质过氧化,提高机体抗氧化能力,防止氧化应激的发生,改善酒精对小鼠造成的脂质代谢紊乱来发挥护肝作用。
To study the protective effect of 4-methyl guaiacol on alcoholic liver injury in mice, fifty mice were randomly divided into five groups: 4-methyl blank group, model group and guaiacol with low, medium and high dose groups. The mice were gavage with the same volume of saline solution, 50% alcohol and low, medium and high dose of 4-methyl guaiacol, experiment lasted eight weeks, the animals were then killed after an overnight fasting of 12 h. After 12 h, blood samples were collected from the eyeballs to determine the concentrations of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and the activities of AST and ALT in serum of mice. Total superoxide dismutase (SOD) activity, reduced glutathione (GSH) concentration, glutathione peroxidase (GSH-Px) activity, catalase (CAT) activity, malondialdehyde (MDA) concentration in liver tissue homogenate were also determined. The concentrations of TC, TG and LDL-C in serum of 4-methylguaiacol treatment groups, as well as the activities of AST and ALT, were lower than those in the model group, and the concentration of HDL-C was higher. SOD activity, CAT activity, GSH-Px activity and GSH concentration in liver tissue homogenate of mice in 4-methylguaiacol treatment groups were higher than those in the model group, while MDA concentration was lower (P<0.05), which was related to 4-methylguaiacol concentration. 4-methylguaiacol had protective effect on alcoholic liver injury and was dose-dependent. 4-methyl guaiacol plays a protective role in liver by reducing liver tissue damage, reducing lipid peroxidation, improving the body′s antioxidant capacity, preventing oxidative stress, and improving the lipid metabolism disorder caused by alcohol in mice.
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