食品与发酵工业 >

2021 , Vol. 47 >Issue 18: 119 - 126

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027306

研究报告

长松藻多糖降解、结构表征及降血糖活性测定

  • 严尚隆 ,
  • 潘创 ,
  • 杨贤庆 ,
  • 陈胜军 ,
  • 戚勃 ,
  • 黄卉
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  • 1(上海海洋大学 食品学院,上海,201306)
    2(中国水产科学研究院南海水产研究所,农业农村部水产品加工重点实验室,国家水产品加工技术研发中心,广东 广州,510300)
硕士研究生(潘创助理研究员和杨贤庆研究员为共同通讯作者,E-mail:silverpfoxc@hotmail.com;yxqgd@163.com)

收稿日期: 2021-03-15

  修回日期: 2021-04-29

  网络出版日期: 2021-10-18

基金资助

广东特色海洋生物制品共性关键技术研发及产业应用(2020B1111030004);国家海藻产业技术体系岗位科学家专项(CARS-50);农业农村部水产品加工重点实验室开放基金(NYJG201906);广东省重点领域研发计划资助(2019B020225001);广东省促进经济发展专项资金(粤农2019B14);中国水产科学研究院基本科研业务费资助(2020TD73)

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Degradation, structural characterization and hypoglycemic activity of polysaccharides from Codium cylindricum

  • YAN Shanglong ,
  • PAN Chuang ,
  • YANG Xianqing ,
  • CHEN Shengjun ,
  • QI Bo ,
  • HUANG Hui
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  • 1(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)
    2(South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs/National Research and Development Center for Aquatic Product Processing, Guangzhou 510300, China)

Received date: 2021-03-15

  Revised date: 2021-04-29

  Online published: 2021-10-18

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摘要

为提高长松藻(Codium cylindricum)多糖的生物活性并扩大长松藻多糖的应用范围,该文采用超声辅助H2O2-维生素C法以及H2O2-Fe2+法降解长松藻多糖,对多糖的理化性质、单糖组成、表观构象及糖苷键构型等进行测定,比较多糖的结构及体外降血糖活性差异。结果表明,降解后的长松藻多糖溶解性显著改善,粒径减小、Zeta电位降低。同时LC-MS、原子力显微镜、核磁共振测定结果表明降解处理后,单糖组成相同(甘露糖、半乳糖、葡萄糖、阿拉伯糖等),糖苷键构型不变(α和β型),而表面结构发生变化(分子高度和聚集程度降低)。体外降血糖活性实验结果表明,长松藻多糖具备一定的α-淀粉酶和α-葡萄糖苷酶抑制活性。降解处理后多糖的α-葡萄糖苷酶抑制能力显著增强,10 mg/mL时最大抑制率分别为47.74%、55.41%、60.07%。该研究为长松藻多糖的生物活性提供优化方向,为长松藻多糖的降解开发利用奠定理论基础。

关键词： 长松藻; 多糖; 降解; 理化性质; 结构解析; 降血糖活性

本文引用格式

严尚隆 , 潘创 , 杨贤庆 , 陈胜军 , 戚勃 , 黄卉 . 长松藻多糖降解、结构表征及降血糖活性测定[J]. 食品与发酵工业, 2021 , 47(18) : 119 -126 . DOI: 10.13995/j.cnki.11-1802/ts.027306

Abstract

To improve the biological activities, ultrasound-assisted H2O2-vitamin C and H2O2-Fe2+ methods were used to degrade the polysaccharides from Codium cylindricum. The physicochemical properties of polysaccharides were determined and the monosaccharide composition, surface conformation and glycosidic bond configuration were analyzed. The physicochemical properties determination indicated that the solubility of polysaccharides was significantly improved after degradation, and the particle size and Zeta potential had been found to decrease. The results of LC-MS, AFM and NMR showed that the degraded polysaccharides were composed of the same monosaccharide units and glycosidic bond configuration with Codium cylindricum polysaccharides. Meanwhile, the surface structure had been changed after degradation. The results of in vitro hypoglycemic activity determination demonstrated that polysaccharides from Codium cylindricum had moderate inhibitory activities of α-amylase and α-glucosidase, and the activities were enhanced after degradation. The obtained results could provide a theoretical foundation for the application of Codium cylindricum degraded polysaccharides.

Key words： Codium cylindricum; polysaccharides; degradation; physicochemical property; structural characterization; hypoglycemic activity

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