食品与发酵工业 >

2021 , Vol. 47 >Issue 18: 159 - 164

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027704

研究报告

核桃青皮提取物对非酒精性脂肪肝的作用

  • 方琼莲 ,
  • 王蒙蒙 ,
  • 乔雪 ,
  • 李欣坪 ,
  • 黄丰 ,
  • 林玉萍 ,
  • 赵声兰
展开
  • (云南中医药大学 中药学院,云南省南药可持续利用重点实验室,云南 昆明,650500)
硕士研究生(林玉萍副教授和赵声兰教授为共同通讯作者,E-mail:11217005@qq.com;13330431529@163.com)

收稿日期: 2021-04-15

  修回日期: 2021-05-14

  网络出版日期: 2021-10-18

基金资助

云南省科技厅中医联合专项(2017FF117-022;2017FF116-016);生物资源数字化开发应用项目(202002AA100007)

收起

Effect of walnut green husk extracts on non-alcoholic fatty liver disease

  • FANG Qionglian ,
  • WANG Mengmeng ,
  • QIAO Xue ,
  • LI Xinping ,
  • HUANG Feng ,
  • LIN Yuping ,
  • ZHAO Shenglan
Expand
  • (School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Kunming 650500, China)

Received date: 2021-04-15

  Revised date: 2021-05-14

  Online published: 2021-10-18

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摘要

采用醇提法提取核桃青皮有效成分,用乙酸乙酯、正丁醇萃取得到不同萃取物。SPF级雄性SD大鼠随机分为正常对照组、模型组、阳性对照组、核桃青皮乙醇提取物组、乙酸乙酯萃取物组、正丁醇萃取物组,给予高脂饲料12周,灌胃给药6周后,检测大鼠血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、谷氨酸氨基转移酶(glutamate aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST);肝组织中丙二醛(malondialdehyde,MDA)含量,超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性;用苏木精-伊红染色法(hematoxylin-eosin staining,HE)进行肝组织病理学切片分析。结果表明,与正常组相比,模型组大鼠TG、TC、HDL-C、ALT、AST和MDA含量显著升高(P＜0.01),LDL-C含量和GSH-Px、SOD活性显著降低(P＜0.001),肝组织病理损伤明显;与模型组相比,核桃青皮乙醇提取物组、乙酸乙酯萃取物组、正丁醇萃取物组的TG、TC、HDL-C、ALT、AST、MDA含量显著降低(P＜0.05),LDL-C含量和GSH-Px、SOD活性显著升高(P＜0.01);肝组织病理损伤有明显改善;其中,正丁醇萃取物效果更好。

关键词： 核桃青皮; 非酒精性脂肪肝; 高脂诱导; 改善肝损伤

本文引用格式

方琼莲 , 王蒙蒙 , 乔雪 , 李欣坪 , 黄丰 , 林玉萍 , 赵声兰 . 核桃青皮提取物对非酒精性脂肪肝的作用[J]. 食品与发酵工业, 2021 , 47(18) : 159 -164 . DOI: 10.13995/j.cnki.11-1802/ts.027704

Abstract

The effective components of walnut green husk were extracted by alcohol extraction, and different extracts were obtained by extraction with ethyl acetate and n-butanol. The effect of different extracts from walnut green husk on non-alcoholic fatty liver disease (NAFLD) were investigated. Specific pathogen free (SPF) male Sprague Dawley (SD) rats were randomly divided into normal control group, model group, positive control group and walnut green husk ethanol, ethyl acetate and n-butanol group. The rats were fed with high-fat diet for 12 weeks and given intragastric administration for 6 weeks. The serum levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), glutamate aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. The content of malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver tissue were checked. Pathological sections were analyzed by hematoxylin-eosin staining (HE). Compared with the normal group, the contents of TG, TC, HDL-C, ALT, AST and MDA in the model group were significantly increased (P<0.01), while the contents of LDL-C, GSH-Px and SOD activities were significantly decreased (P<0.001), and the pathological damage of liver tissue was obvious. Compared with the model group, the contents of TG, TC, HDL-C, ALT, AST and MDA in the ethanol, ethyl acetate and n-butanol extracts of walnut green husk decreased significantly (P<0.05), while the contents of LDL-C and the activities of GSH-Px and SOD increased significantly (P<0.01). Pathological damage was improved obviously. The n-butanol extract had more obvious effect than other extracts.

Key words： walnut green husk; NAFLD; HFD-induced; improving liver injury

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