研究报告

两歧双歧杆菌F35氮源利用的差异性解析

  • 汪文丽 ,
  • 崔树茂 ,
  • 唐鑫 ,
  • 毛丙永 ,
  • 赵建新 ,
  • 陈卫
展开
  • (江南大学 食品学院,江苏 无锡,214122)
硕士研究生(崔树茂副研究员为通讯作者,E-mail:cuishumao@jiangnan.edu.cn)

收稿日期: 2020-12-03

  修回日期: 2021-01-06

  网络出版日期: 2021-11-04

基金资助

国家食品科学与工程一流学科建设项目(JUFSTR20180102);国家青年科学基金项目(31801530)

Various nitrogen source utilization in Bifidobacterium bifidum F35

  • WANG Wenli ,
  • CUI Shumao ,
  • TANG Xin ,
  • MAO Bingyong ,
  • ZHAO Jianxin ,
  • CHEN Wei
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  • (School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

Received date: 2020-12-03

  Revised date: 2021-01-06

  Online published: 2021-11-04

摘要

为研究两歧双歧杆菌氮源利用的特征,并解析其机制,首先测定菌株利用单位质量不同氮源的增殖效果,并通过高效液相色谱测定不同氮源的氨基酸和肽组成,进一步分析了氨基酸氮源和多肽氮源对菌株增殖的影响,最后通过全基因组测序分析菌株的氨基酸转运及生物合成能力。结果表明,两歧双歧杆菌几乎不利用酵母浸粉类氮源,偏好利用蛋白胨,原因在于两歧双歧杆菌利用多肽的增殖效率高于氨基酸,而酵母浸粉类氮源中游离氨基酸含量为其他氮源的2~5倍,但2 000 Da以下小分子肽含量仅为其他氮源的一半;大量疏水性氨基酸的存在会抑制菌株以氨基酸为氮源的增殖,但不会影响菌株对多肽的利用;该菌株具有17种氨基酸的合成能力和丰富的氨基酸转运系统,其基因型及表型均表现为半胱氨酸营养缺陷型。该研究为今后指导两歧双歧杆菌相关氮源的生产提供了理论指导。

本文引用格式

汪文丽 , 崔树茂 , 唐鑫 , 毛丙永 , 赵建新 , 陈卫 . 两歧双歧杆菌F35氮源利用的差异性解析[J]. 食品与发酵工业, 2021 , 47(19) : 21 -28 . DOI: 10.13995/j.cnki.11-1802/ts.026359

Abstract

To study the features of nitrogen source utilization and mechanism of Bifidobacterium bifidum F35. The proliferation effects, the amino acid and peptide composition, the influence of amino acid nitrogen sources and peptide nitrogen sources on the proliferation, the amino acid transport and biosynthesis capacity of B. bifidum were analyzed. The results showed that B. bifidum used little yeast extract, preferring to use peptone; because B. bifidum used peptides more efficiently than amino acids, and the free amino acid of yeast extract were 2-5 times than that of other nitrogen sources, but the content of small molecule peptides below 2 000 Da were only half of others. The presence of a large number of hydrophobic amino acids would inhibit the growth of strains, but not affect the strain's utilization of polypeptides; it had the ability to synthesize 17 amino acids and rich amino acid transport system. Its genotype and phenotype were both cysteine auxotroph. The results provide theoretical guidance for the future production of nitrogen sources related to B.bifidum

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