该文通过水蒸馏法提取天祝祁连圆柏精油,采用GC-MS分析精油成分,并通过滤纸片熏蒸法和最低抑菌浓度(minimal inhibitory concentration, MIC)法、最低杀菌浓度(minimal,fungicidal concentration,MFC)法研究祁连圆柏精油的抑菌活性。结果表明,祁连圆柏精油得率为2.83%,从中共检测出47种化学成分,其中相对含量较高的物质为桧烯(19.59%)、α-杜松醇(11.81%)、(-)-4-萜品醇(10.90%)、γ-松油烯(5.55%)、香茅醇(5.06%)、萜品油烯(3.58%)、月桂烯(3.705%)、D-柠檬烯(3.34%)等物质。祁连圆柏精油对4种果蔬腐败真菌的抑菌活性存在显著浓度依赖性,对扩展青霉(Penicillium expansum)和硫色镰刀菌(Fusarium sulphureum)的MIC均为0.875 μL/mL,MFC均为1 μL/mL;互隔交链孢(Alternaria alternata)和粉红单端孢(Trichosporon roseum)的MIC均为1.125 μL/mL, MFC均为1.25 μL/mL。金黄色葡萄球菌(Staphylococcus aureus)和枯草芽孢杆菌(Bacillus subtilis)的MIC均为2.34 μL/mL,最低杀细菌浓度(minimun bactericidal concentration,MBC)均为4.68 μL/mL;粪产碱杆菌(Alcaligenes faecalis)和大肠杆菌(Escherichia coli)的MIC均为4.68 μL/mL,MBC分别为4.68、9.36 μL/mL。该研究表明祁连圆柏有较高的精油含量,精油成分丰富且对真菌和细菌均有较好的抑制活性,为祁连圆柏精油的开发和利用提供了依据。
Plant essential oil is considered as a good potential substitute in antibacteria field since it has many superior characteristics. Due to rich essential oil content, Cupressaceae plant had aroused wide attention. In the present study, the essential oil of Sabina przewalskii Kom. was extracted by hydro-distillation, and its components were analysed by gas chromatography-mass spectrometry. The antibacterial activity of S. przewalskii essential oil was studied by filter paper fumigation, minimum inhibitory concentration (MIC) method and minimum bactericidal /fungicidal concentration method(MBC/MFC). The results showed that the yield of S. przewalskii essential oil was 2.83%. Forty-seven chemical constituents were identified in the essential oil, among which 4(10)-arborvitae (19.59%), α-juniperol (11.81%), (-)-4- terpineol (10.90%), and γ-terpineol (5.55%), citronellol (5.06%), terpinolene (3.58%), myrcene (3.705%), D-limonene (3.34%) were the most abundant compounds. The antimicrobial activity of S. przewalskii essential oil had significant concentration-dependent on four spoilage fungi of fruit and vegetables. The MIC of the essential oil on both Penicillium expansum and Fusarium sulphureum was 0.875 μL/mL, and the MFC on both fungi was 1 μL/mL. The MIC of the essential oil on both Alternaria alternata and Trichosporon roseum was 1.125 μL/mL, and the MFC was 1.25 μL/mL. The MIC of the essential oil on Staphylococcus aureus and Bacillus subtilis was 2.34 μL/mL, and the MBC was 4.68 μL/mL. The MIC of the essential oil on Alcaligenes faecalis and Escherichia coli was 4.68 μL/mL, and the MBC was 9.36 and 4.68 μL/mL, respectively. This study showed that S. przewalskii had a high content of essential oil, which had good inhibitory activity on fungi and bacteria. It provides a basis for S. przewalskii development and utilization.
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