食品与发酵工业 >

2021 , Vol. 47 >Issue 20: 120 - 125

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026675

研究报告

罗非鱼鳞胶原肽螯合锌抗氧化及抑菌活性研究

  • 郭洪辉 ,
  • 陈晖 ,
  • 赵雪 ,
  • 方华 ,
  • 洪专
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  • (自然资源部第三海洋研究所 海洋生物资源开发利用工程技术创新中心,福建 厦门,361005)
博士,副研究员(洪专教授级高级工程师为通讯作者,E-mail:zhong@tio.org.cn)

收稿日期: 2021-01-06

  修回日期: 2021-02-19

  网络出版日期: 2021-11-18

基金资助

福建省科技计划引导性项目(2019N0033);厦门海洋高技术产业基地创业创新共享服务平台项目(16PFW008SF15);“十三五”北海市海洋经济创新发展示范项目(Bhsfs009)

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Antioxidant and antibacterial activities of collagen peptide chelated zinc from tilapia scales

  • GUO Honghui ,
  • CHEN Hui ,
  • ZHAO Xue ,
  • FANG Hua ,
  • HONG Zhuan
Expand
  • (Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography,Ministry of Natural Resources, Xiamen 361005, China)

Received date: 2021-01-06

  Revised date: 2021-02-19

  Online published: 2021-11-18

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摘要

以罗非鱼鳞为原料,制备了胶原肽及胶原肽螯合锌,研究并比较了其体外抗氧化及抑菌活性。基质辅助激光解析电离飞行时间质谱图表明了罗非鱼鳞胶原肽螯合锌的生成。氨基酸分析显示,罗非鱼鳞胶原肽富含羟脯氨酸、甘氨酸以及天冬氨酸,与锌离子螯合后,氨基酸的组成与比例有所变化,其中天冬氨酸与谷氨酸占总氨基酸的比例分别由19.72%和8.79%增加到22.73%和9.71%。体外抗氧化实验表明,罗非鱼鳞胶原肽螯合锌对DPPH自由基、羟自由基的清除率IC50 值分别为3.69和6.59 g/L,并且具有较强的Fe3+还原能力,螯合锌的抗氧化活性明显优于胶原肽。抑菌实验显示罗非鱼鳞胶原肽螯合锌对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌和白色假丝酵母等4种指示菌均表现出明显的抑制作用,而罗非鱼鳞胶原肽无抑菌效果。由此可见,罗非鱼鳞胶原肽螯合锌有望作为一种潜在的抗氧化、抑菌剂。

关键词： 罗非鱼鳞; 胶原肽螯合锌; 氨基酸分析; 抗氧化; 抑菌活性

本文引用格式

郭洪辉 , 陈晖 , 赵雪 , 方华 , 洪专 . 罗非鱼鳞胶原肽螯合锌抗氧化及抑菌活性研究[J]. 食品与发酵工业, 2021 , 47(20) : 120 -125 . DOI: 10.13995/j.cnki.11-1802/ts.026675

Abstract

Collagen peptide and collagen peptide chelated zinc were prepared from tilapia scales and evaluated in terms of their in vitro antioxidant and antibacterial activities. The matrix assisted laser desorption ionization time-of-flight mass spectra confirmed the generation of collagen peptide chelated zinc. Amino acid analysis indicated that the collagen peptide was rich in hydroxyproline, glycine and aspartic acid. After chelation with zinc ions, the amino acid composition and proportion were significantly changed. Especially, the proportion of aspartic acid and glutamic acid in total amino acids increased from 19.72% and 8.79% to 22.73% and 9.71%, respectively. In vitro antioxidant results showed that the IC50 values of collagen peptide chelated zinc were 3.69 and 6.59 g/L for DPPH and ·OH free radical scavenging ability, respectively. Furthermore, collagen peptide chelated zinc displayed strong ferric iron reducing power. The antioxidant activity of collagen peptide chelated zinc was superior to that of collagen peptide. Although collagen peptide from tilapia scales showed no antibacterial activity, collagen peptide chelated zinc exhibited significant antibacterial activity against Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Candida albicans. Therefore, collagen peptide chelated zinc from tilapia scales could be used as a potential antioxidant and antibacterial agent.

Key words： tilapia scale; collagen peptide chelated zinc; amino acid analysis; antioxidant; antibacterial activity

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