为了研究益生菌联合益生元对肥胖的改善效果及其潜在作用机制,将植物乳杆菌(Lactobacillus plantarum)F3-2与甘露低聚糖(mannooligosaccharides,MOS)联合作用,干预高脂饮食诱导的肥胖小鼠。实验结束后,通过分析体质量、脂肪组织质量、血清脂质水平等指标,对益生菌与益生元联合作用后的效果进行评价。进一步对脂肪组织中脂代谢关键基因及小鼠粪便短链脂肪酸(short-chain fatty acids,SCFAs)水平进行检测分析,探究二者联合作用机制。结果表明,L.plantarum F3-2联合MOS作用能有效减轻小鼠体质量,此外脂肪组织质量、脂肪细胞大小及血清中甘油三酯含量相比MOS单独作用时均显著降低。两者联合作用有效提升了小鼠粪便中SCFAs特别是丁酸的水平,并增强了MOS对脂肪组织中脂代谢关键基因过氧化物酶体增殖物激活受体-γ(peroxisome proliferator-activated receptor-γ,PPARγ)及CCAAT/增强子结合蛋白-α(CCAAT/enhancer binding protein-α,C/EBPα)的抑制作用。L.plantarum F3-2与MOS有望作为合生元通过干预饮食结构改善肥胖病症。
赵茂臻
,
梁曦
,
张喆
,
吕优优
,
王引
,
刘同杰
,
易华西
,
公丕民
,
张兰威
. 植物乳杆菌F3-2联合甘露低聚糖通过上调短链脂肪酸水平抑制PPARγ改善小鼠肥胖[J]. 食品与发酵工业, 2021
, 47(23)
: 78
-82
.
DOI: 10.13995/j.cnki.11-1802/ts.027495
To explore the possible effect of probiotics and prebiotics on obesity, Lactobacillus plantarum F3-2 and mannooligosaccharides (MOS) to intervene in obese mice. The critical genes of lipid metabolism in adipose tissue and the levels of short-chain fatty acids (SCFAs) in mouse feces were analyzed. The results showed that L. plantarum F3-2 combined with MOS could effectively reduce the body weight of mice, the weight of adipose tissue, the size of adipocytes and the content of TG in serum compared with MOS alone. The levels of SCFAs, especially butyric acid, in mouse feces effectively increased. The inhibitory effect of MOS on critical genes of lipid metabolism in adipose tissue, including peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer binding protein-α(C/EBPα), were enhanced. L. plantarum F3-2 and MOS are expected to act as symbiotic to intervene in dietary structure and improve obesity.
[1] 王洪善,张晓娟,李恒,等.甘露寡糖对高脂饮食小鼠的益生作用[J].食品与发酵工业,2018,44(11):63-68.
WANG H S,ZHANG X J,LI H,et al.Prebiotic effects of mannan-oligosaccharide on high fat diet mice[J].Food and Fermentation Industries,2018,44(11):63-68.
[2] KUMAO T,FUJII S,OZAKI K,et al.Effect of diets with mannooligosaccharides from coffee mannan on fat in blood serum in healthy volunteers[J].Japan Journal of Food Engineering,2005,6(4):301-304.
[3] ST-ONGE M P,SALINARDI T,HERRON-RUBIN K,et al.A weight-loss diet including coffee-derived mannooligosaccharides enhances adipose tissue loss in overweight men but not women[J].Obesity,2012,20(2):343-348.
[4] ASANO I,FUJII S,OZAKI K,et al.Effects of “coffee beverage” containing mannooligosaccharides from coffee on human abdominal fat by long term ingestion[J].Japan Journal of Food Engineering,2005,6(2):133-141.
[5] AN H M,PARK S Y,LEE D K,et al.Antiobesity and lipid-lowering effects of Bifidobacterium spp.in high fat diet-induced obese rats[J].Lipids in Health and Disease,2011,10:116.
[6] WU C C,WENG W L,LAI W L,et al.Effect of Lactobacillus plantarum strain K21 on high-fat diet-fed obese mice[J].Evidence-Based Complementary and Alternative Medicine,2015,2015:391767.
[7] PARK J E,OH S H,CHA Y S.Lactobacillus plantarum LG42 isolated from gajami sik-hae decreases body and fat pad weights in diet-induced obese mice[J].Journal of Applied Microbiology,2014,116(1):145-156.
[8] 李亚男,马晓峰,王小英,等.乳杆菌联合甘露寡糖对非酒精性小鼠脂肪肝的影响[J].广东医学,2019,40(3):344-347.
LI Y N,MA X F,WANG X Y,et al.Effect of Lactobacillus and manna oligosaccharides on non-alcoholic fatty liver disease in mice[J].Guangdong Medical Journal,2019,40(3):344-347.
[9] POURABEDIN M,CHEN Q L,YANG M M,et al.Mannan-and xylooligosaccharides modulate caecal microbiota and expression of inflammatory-related cytokines and reduce caecal Salmonella Enteritidis colonisation in young chickens[J].FEMS Microbiology Ecology,2017,93(1):fiw226.
[10] LIANG X,LV Y,ZHANG Z,et al.Study on intestinal survival and cholesterol metabolism of probiotics[J].LWT,2020,124:109 132.
[11] PARK S,JI Y,JUNG H Y,et al.Lactobacillus plantarum HAC01 regulates gut microbiota and adipose tissue accumulation in a diet-induced obesity murine model[J].Applied Microbiology and Biotechnology,2017,101(4):1 605-1 614.
[12] SATO M,UZU K,YOSHIDA T,et al.Effects of milk fermented by Lactobacillus gasseri SBT2055 on adipocyte size in rats[J].The British Journal of Nutrition,2008,99(5):1 013-1 017.
[13] LIANG X,ZHANG Z,LV Y,et al.Reduction of intestinal trimethylamine by probiotics ameliorated lipid metabolic disorders associated with atherosclerosis[J].Nutrition,2020,79-80:110 941.
[14] ZHANG J,TANG H J,ZHANG Y Q,et al.Identification of suitable reference genes for quantitative RT-PCR during 3T3-L1 adipocyte differentiation[J].International Journal of Molecular Medicine,2014,33(5):1 209-1 218.
[15] PARK J E,OH S H,CHA Y S.Lactobacillus plantarum LG42 isolated from gajami sik-hae inhibits adipogenesis in 3T3-L1 adipocyte[J].BioMed Research International,2013,2013:460 927.
[16] WANG T,YAN H,LU Y Y,et al.Anti-obesity effect of Lactobacillus rhamnosus LS-8 and Lactobacillus crustorum MN047 on high-fat and high-fructose diet mice base on inflammatory response alleviation and gut microbiota regulation[J].European Journal of Nutrition,2020,59(6):2 709-2 728.
[17] CHAUHAN P S,SHARMA P,PURI N,et al.Purification and characterization of an alkali-thermostable β-mannanase from Bacillus nealsonii PN-11 and its application in mannooligosaccharides preparation having prebiotic potential[J].European Food Research and Technology,2014,238(6):927-936.
[18] GUO Y,HU Y,BAO W.Effects of konjac mannose-oligosaccharides in diets on immunity function and mecum microflora of three-yellow broilers’[J].Chinese Agricultural Science Bulletin,2011,27(17):58-61.
[19] DE VADDER F,KOVATCHEVA-DATCHARY P,GONCALVES D,et al.Microbiota-generated metabolites promote metabolic benefits via gut-brain neural circuits[J].Cell,2014,156(1-2):84-96.
[20] ASANO I,IKEDA Y,FUJII S,et al.Effects of mannooligosaccharides from coffee on microbiota and short chain fatty acids in rat cecum[J].Food Science and Technology Research,2004,10(3):273-277.
[21] DEN BESTEN G,BLEEKER A,GERDING A,et al.Short-chain fatty acids protect against high-fat diet-induced obesity via a PPARγ-dependent switch from lipogenesis to fat oxidation[J].Diabetes,2015,64(7):2 398-2 408.
