食品与发酵工业 >

2021 , Vol. 47 >Issue 23: 112 - 117

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027501

研究报告

常压室温等离子体诱变选育抗后酸化的乳酸菌

  • 周虹瑾 ,
  • 霍向东 ,
  • 赵丹 ,
  • 林青 ,
  • 娄恺 ,
  • 袁华伟
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  • 1(新疆大学 生命科学与技术学院,新疆 乌鲁木齐,830000)
    2(新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,新疆 乌鲁木齐,830091)
    3(宜宾学院 质量管理与检验检测学部/固态发酵资源利用四川省重点实验室,四川 宜宾,644000)
博士研究生(袁华伟研究员为通讯作者,E-mail: 843204747@qq.com)

收稿日期: 2021-03-24

  修回日期: 2021-05-21

  网络出版日期: 2021-12-31

基金资助

兵团重点研发计划专项(2020AB013);固态发酵资源利用四川省重点实验室开放基金项目(2018GTJ014);宜宾学院科研项目(2020YY04)

收起

Screening of lactic acid bacteria with anti-postacidification by atmospheric and room temperature plasma mutagenesis

  • ZHOU Hongjin ,
  • HUO Xiangdong ,
  • ZHAO Dan ,
  • LIN Qing ,
  • LOU Kai ,
  • YUAN Huawei
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  • 1(College of Life Science and Technology,Xinjiang University,Urumqi 830000,China)
    2(Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences/Xinjiang Laboratory of Special Environmental Microbiology,Urumqi 830091,China)
    3(Facuity of quality management and inspection quarantine/Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province,Yibin University,Yibin 644000,Sichuan)

Received date: 2021-03-24

  Revised date: 2021-05-21

  Online published: 2021-12-31

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摘要

为降低酸奶在运输及储藏期间的后酸化,采用常压室温等离子体(atmospheric and room temperature plasma,ARTP)和新霉素诱变筛选,通过致死率曲线与阳性筛选率曲线确定最佳处理条件为ARTP处理30 s,新霉素筛选质量浓度为300 mg/mL,该条件下共获得7株生长特性良好,具有较低H+-ATP酶活性的副干酪乳杆菌(Lactobacillus paracasei)突变株。通过全脂牛乳发酵,突变菌株BJT-7在20 d储藏期间酸度维持在70 °T以下,遗传稳定性良好。结果表明,ARTP是一种高效诱变方法,结合新霉素筛选可得到抗后酸化能力强的乳酸菌株。

关键词： 抗后酸化; 常压室温等离子体诱变; 副干酪乳杆菌; 新霉素

本文引用格式

周虹瑾 , 霍向东 , 赵丹 , 林青 , 娄恺 , 袁华伟 . 常压室温等离子体诱变选育抗后酸化的乳酸菌[J]. 食品与发酵工业, 2021 , 47(23) : 112 -117 . DOI: 10.13995/j.cnki.11-1802/ts.027501

Abstract

In order to reduce the post-acidification problem of yogurt during transportation and storage, atmospheric and room temperature plasma (ARTP) and neomycin mutagenesis were used. According to the lethality curve and the positive screening rate curve, the best treatment conditions were determined as follows: ARTP irradiation time 30 s, neomycin screening concentration was 300 mg/mL. Seven mutant strains of Lactobacillus paracasei with good growth characteristics and low H+-ATPase activity were obtained. Through the fermentation of whole milk, the acidity of the mutant strain BJT-7 was maintained below 70 °T during the 20 d of storage, and the genetic stability was good. The results showed that ARTP was an efficient mutagenesis method, combined with neomycin screening can obtain lactic acid strains with strong resistance to post-acidification.

Key words： anti-postacidification; atmospheric and room temperature plasma mutagenesis; Lactobacillus paracasei; neomycin

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