食品与发酵工业 >

2021 , Vol. 47 >Issue 23: 252 - 257

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027884

分析与检测

分散固相萃取-气相色谱法测定鸡蛋中氟虫腈及其代谢物

  • 郝莉花 ,
  • 贾利平
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  • 1(河南省产品质量监督检验院,河南 郑州,450047)
    2(河南省食品安全数据智能重点实验室,河南 郑州,450047)
高级工程师(通讯作者,E-mail:330767838@163.com)

收稿日期: 2021-05-04

  修回日期: 2021-08-11

  网络出版日期: 2021-12-31

基金资助

河南省重点研发与推广专项(科技攻关)项目(202102310295);河南省重点研发与推广专项(科技攻关)项目(212102410005);河南省市场监督管理局科技计划项目(2020sj22)

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Determination of fipronil and its metabolites in eggs by QuEChERS combined with gas chromatography

  • HAO Lihua ,
  • JIA Liping
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  • 1(Henan Institute of Quality Supervision and Inspection,Zhengzhou 450047,China)
    2(Henan key laboratory of food safety data intelligence,Zhengzhou 450047,China)

Received date: 2021-05-04

  Revised date: 2021-08-11

  Online published: 2021-12-31

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摘要

采用气相色谱法对鸡蛋中氟虫腈及其代谢物(氟甲腈、氟虫腈亚砜和氟虫腈砜)进行了检测。试样经QuEChERS净化、HP-5弹性石英柱分离,外标法定量,氟虫腈、氟甲腈、氟虫腈亚砜和氟虫腈砜4种物质在0.005~0.2 μg/mL线性良好。采用该方法,氟虫腈、氟甲腈、氟虫腈亚砜和氟虫腈砜的检出限均为0.01 mg/kg,且在0.025、0.4、1.0 mg/kg水平下进行加标回收实验,氟虫腈、氟甲腈、氟虫腈亚砜和氟虫腈砜平均回收率为88.8%~115.1%,相对标准偏差为0.86%~5.22%。

关键词： 气相色谱法; 鸡蛋; 氟虫腈; 代谢物

本文引用格式

郝莉花 , 贾利平 . 分散固相萃取-气相色谱法测定鸡蛋中氟虫腈及其代谢物[J]. 食品与发酵工业, 2021 , 47(23) : 252 -257 . DOI: 10.13995/j.cnki.11-1802/ts.027884

Abstract

A gas chromatograph method for the determination of fipronil and its metabolites in eggs was developed. The samples were cleaned up by QuEChERS, separated on an HP-5 flexible quartz column, and quantified by a standard external method. The four fipronil's linearity and its metabolites were acceptable in the range of 0.005-0.2 μg/mL. The detection limits and limits of quantification of the four fipronil and its metabolites were 0.01 μg/mL and 0.025 mg/kg, respectively. The average recoveries were 88.8%-115.1% at the levels of 0.025, 0.4, and 1.0 mg/kg which with the relative standard deviation(n=3) of 0.86%-5.22%.

Key words： gas chromatography; eggs; fipronil; metabolites

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