食品与发酵工业 >

2021 , Vol. 47 >Issue 24: 48 - 55

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027169

研究报告

漠河蓝果忍冬多酚成分鉴定及其体外生物活性分析

  • 朱冠冰 ,
  • 乔锦莉 ,
  • 呼延文静 ,
  • 刘佩 ,
  • 张妍 ,
  • 霍俊伟
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  • 1(寒地小浆果开发利用国家地方联合工程研究中心,黑龙江 哈尔滨,150030)
    2(东北农业大学 园艺园林学院,黑龙江 哈尔滨,150030)
    3(东北农业大学 农业部东北地区园艺作物生物学与种质创制重点实验室,黑龙江 哈尔滨,150030)
硕士研究生(张妍副教授和霍俊伟教授为共同通讯作者,E-mail:zhang_yan@neau.edu.cn;huojunwei@neau.edu.cn)

收稿日期: 2021-02-26

  修回日期: 2021-04-14

  网络出版日期: 2022-01-21

基金资助

国家自然科学基金项目(31801553);国家重点研发计划项目(2016YFC500304-05);黑龙江省自然科学基金项目(C2017020;YQ2020C011)

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Identification and in vitro biological activity analysis of polyphenolic from Mohe blue honeysuckle

  • ZHU Guanbing ,
  • QIAO Jinli ,
  • HUYAN Wenjing ,
  • LIU Pei ,
  • ZHANG Yan ,
  • HUO Junwei
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  • 1(National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, Harbin 150030, China)
    2(College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China)
    3(Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, China)

Received date: 2021-02-26

  Revised date: 2021-04-14

  Online published: 2022-01-21

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摘要

该文以漠河蓝果忍冬果实为研究对象,采用高效液相色谱-电喷雾质谱对其花色苷和非花色苷多酚的组分进行分离鉴定,并且分析其抗氧化活性、抗脂肪酶活性、抗α-淀粉酶活性及抗α-葡萄糖苷酶活性。结果表明,漠河市蓝果忍冬果实中含有8种花色苷,其中矢车菊-3-葡萄糖苷所占总和的比例最大,而24种非花色苷多酚中5-咖啡酰奎宁酸含量最高。漠河蓝果忍冬总酚含量高达(108.52±6.80) mg GAE/100g DW,总花青素含量为(23.36±0.54) mg C3G/100g DW。在抗氧化方面具有较强的生物活性:清除DPPH自由基的能力为(201.19±9.97) μmol TE/g DW、铁离子还原能力为(5.16±0.16) μmol TE/g DW、总抗氧化能力为(658.18±9.73) μmol TE/g DW、抗氧化能力为(414.79±2.08) μmol TE/g DW,并且在抑制碳水化合物水解酶(抑制α-淀粉酶的IC50值为6.399 mg/mL、抑制α-葡萄糖苷酶的IC50值为11.73 mg/mL)和脂肪酶(IC50值为1.912 mg/mL)方面也表现出一定的生物活性。

关键词： 蓝果忍冬; 多酚; 抗氧化活性; 抗淀粉酶活性; 抗脂肪酶活性

本文引用格式

朱冠冰 , 乔锦莉 , 呼延文静 , 刘佩 , 张妍 , 霍俊伟 . 漠河蓝果忍冬多酚成分鉴定及其体外生物活性分析[J]. 食品与发酵工业, 2021 , 47(24) : 48 -55 . DOI: 10.13995/j.cnki.11-1802/ts.027169

Abstract

In this study, the anthocyanins and non anthocyanin polyphenols of Mohe blue honeysuckle were isolated and identified by high performance liquid chromatography-electrospray tandem mass spectrometry (HPLC-ESI-MS2), and their antioxidant activity, lipase inhibition activity, α-amylase inhibition activity, and α-glucosidase inhibition activity were analyzed. The results showed that blue honeysuckle fruits in Mohe contained 8 kinds of anthocyanins, among which the proportion of cyanidin-3-O-glucoside was significantly high, and the content of 5-caffeoylquinic acid was the highest among 24 kinds of non-anthocyanins. The contents of the total phenolics and the total anthocyanins in crude extracts of blue honeysuckle fruits in Mohe reached (108.52±6.80) mg GAE/100g DW and (23.36±0.54) mg C3G/100g DW, which showed strong antioxidant activity (201.19±9.97) μmol TE/g DW, (5.16±0.16) μmol TE/g DW, (658.18±9.73) μmol TE/g DW, and (414.79±2.08) μmol TE/g DW against DPPH, FRAP, ABTS, and ORAC assays, respectively. The results also showed certain biological activity of the inhibition activity of α-amylase, α-glucosidase and lipase. The semi-inhibitory concentrations of α-amylase, α-glucosidase and lipase were 6.399 mg/mL, 11.73 mg/mL and 1.912 mg/mL, respectively. The results of this study provides scientific references and valuable insights for blue honeysuckle fruit in Mohe.

Key words： blue honeysuckle; polyphenols; antioxidant activity; starch hydrolase inhibition activity; lipase inhibition activity

参考文献

[1] 王苒, 杜鹏飞, 李明文.蓝靛果忍冬新品种比较试验研究[J].安徽农学通报, 2019, 25(13):32-35.
WANG R, DU P F, LI M W.Breeding research of the fine variety of Lonicera caerulea[J].Anhui Agricultural Science Bulletin, 2019, 25(13):32-35.
[2] 张星, 毕金峰, 陈芹芹, 等.4种浆果成分分析及抗氧化活性研究[J].食品科技, 2020, 45(6):52-58.
ZHANG X, BI J F, CHEN Q Q, et al.Components analysis and antioxidant activities of four varieties of berries[J].Food Science and Technology, 2020, 45(6):52-58.
[3] 朱力国, 郭阳, 唐思媛, 等.不同品种蓝靛果化学成分及抗氧化活性比较[J].中国酿造, 2018, 37(10):153-157.
ZHU L G, GUO Y, TANG S Y, et al.Comparison of chemical constituents and antioxidant activities of different Lonicera caerulea varieties[J].China Brewing, 2018, 37(10):153-157.
[4] KIM J W, LEE Y S, SEOL D J, et al.Anti-obesity and fatty liver-preventing activities of Lonicera caerulea in high-fat diet-fed mice[J].International Journal of Molecular Medicine, 2018, 42(6):3 047-3 064.
[5] ZHOU L P, WANG H, YI J J, et al.Anti-tumor properties of anthocyanins from Lonicera caerulea ‘Beilei’ fruit on human hepatocellular carcinoma:In vitro and in vivo study[J].Biomedicine & Pharmacotherapy, 2018, 104(5):520-529.
[6] PACE E, JIANG Y Y, CLEMENS A, et al.Impact of thermal degradation of cyanidin-3-O-glucoside of haskap berry on cytotoxicity of hepatocellular carcinoma HepG2 and breast cancer MDA-MB-231 cells[J].Antioxidants, 2018, 7(2):24.
[7] ZHAO J, LIN Y, ZHAO Y B, et al.Polyphenol-rich blue honeysuckle extract alleviates silica particle-induced inflammatory responses and macrophage apoptosis via NRF2/HO-1 and MAPK signaling[J].Journal of Functional Foods, 2018, 46(6):463-474.
[8] 杨文康, 李超, 喻柯柯, 等.罗汉果皂甙粗提物的α-葡萄糖苷酶体外抑制活性研究[J].食品工业科技, 2016, 37(24):111-115.
YANG W K, LI C, YU K K, et al.Inhibitory effect of mogrosides crude extract on α-glucosidase in vitro[J].Science and Technology of Food Industry, 2016, 37(24):111-115.
[9] 高媛. 岩高兰多酚的提取纯化、成分鉴定及其生物活性研究[D].哈尔滨:东北林业大学, 2020.
GAO Y.Study on extraction, purification, identification and bioactivities of polyphenols from Empetrum nigrum aerial parts[D].Harbin:Northeast Forestry University, 2020.
[10] SONG H H, BI J F, CHEN Q Q, et al.Structural and health functionality of dried goji berries as affected by coupled dewaxing pre-treatment and hybrid drying methods[J].International Journal of Food Properties, 2018, 21(1):2 527-2 538.
[11] PETROVA K, IVANOVA P, MIHALEV K, et al., Antioxidant capacity, polyphenol and anthocyanin content in blackberries[J].Journal of Mountain Agriculture on the Balkans, 2016,19(2):221-232.
[12] KIM D, LEE C Y.Extraction and isolation of polyphenolics[J].Current Protocols in Food Analytical Chemistry, 2002, 6(1):1 210-1 212.
[13] BRANDWILLIAMS W, CUVELIER M, BERSET C, et al.Use of a free radical method to evaluate antioxidant activity[J].LWT-Food Science and Technology, 1995, 28(1):25-30.
[14] HUANG D J, OU B X, HAMPSCH-WOODILL M, et al.High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format[J].Journal of Agricultural and Food Chemistry, 2002, 50(16):4 437-4 444.
[15] NAYAK B, BERRIOS J D J, POWERS J R, et al.Thermal degradation of anthocyanins from purple potato (Cv.Purple Majesty) and impact on antioxidant capacity[J].Journal of Agricultural and Food Chemistry, 2011, 59(20):11 040-11 049.
[16] RE R, PELLEGRINI N, PROTEGGENTE A, et al.Antioxidant activity applying an improved ABTS radical cation decolorization assay[J].Free Radical Biology and Medicine, 1999, 26(9-10):1 231-1 237.
[17] LIU T T, SONG L X, WANG H Y, et al.A high-throughput assay for quantification of starch hydrolase inhibition based on turbidity measurement[J].Journal of Agricultural & Food Chemistry, 2011, 59(18):9 756-9 762.
[18] WANG Y H,ZHU J Y,MENG X J,et al.Comparison of polyphenol, anthocyanin and antioxidant capacity in four varieties of Lonicera caerulea berry extracts[J].Food Chemistry, 2016, 197:522-529.
[19] YU R,CHEN L,XIN X L.Comparative assessment of chemical compositions,antioxidant and antimicrobial activity in ten berries grown in China[J].Flavour & Fragrance Journal, 2020, 35(2):197-208.
[20] 刘佩, 张妍, 霍俊伟, 等.五种寒地果树果实的多酚含量、抗氧化活性及抗α-淀粉酶活性分析[J].食品工业科技, 2020, 41(4):282-288.
LIU P,ZHANG Y,HUO J W, et al.Analysis of polyphenolic content, antioxidant capacity and α-amylase inhibitory activity of five fruits[J].Science and Technology of Food Industry, 2020, 41(4):282-288.
[21] AUZANNEAU N, WEBER P, KOSINSKA A, et al.Bioactive compounds and antioxidant capacity of Lonicera caerulea,berries:Comparison of seven cultivars over three harvesting years[J].Journal of Food Composition & Analysis, 2017, 66(12):81-89.
[22] RUPASINGHE H P V, ARUMUGGAM N, AMARARATHNA M, et al.The potential health benefits of haskap (Lonicera caerulea L.):Role of cyanidin-3-O-glucoside[J].Journal of Functional Foods, 2018, 44(5):24-39.
[23] MCDOUGALL G J, KULKARNI N N, STEWART D.Berry polyphenols inhibit pancreatic lipase activity in vitro[J].Food Chemistry, 2009, 115(1):193-199.
[24] KITHMA A B, SILVA H D, RUPASINGHE H P V.Polyphenols composition and anti-diabetic properties in vitro of haskap (Lonicera caerulea L.) berries in relation to cultivar and harvesting date[J].Journal of Food Composition and Analysis, 2020, 88(12):10 340.
[25] CAPRIOLI G, IANNARELLI R, INNOCENTI M, et al.Blue honeysuckle fruit (Lonicera caerulea L.) from eastern Russia:Phenolic composition, nutritional value and biological activities of its polar extracts [J].Food & Function, 2016, 7(4):1 892-1 903.
[26] SENICA M, BAVEC M, STAMPAR F, et al.Blue honeysuckle (Lonicera caerulea subsp.edulis (Turcz.Ex Herder) Hultén.) berries and changes in their ingredients across different locations[J].Journal of the Science of Food & Agriculture, 2018, 98(9):3 333-3 342.
[27] DEL BO′ C, MARTINI D, PORRINI M, et al.Berries and oxidative stress markers:An overview of human intervention studies[J].Food & Function, 2015, 6(9):2 890-2 917.
[28] CHEN H J, CHEN B H, INBARAJ B S.Determination of phenolic acids and flavonoids in Taraxacum formosanum Kitam by liquid chromatography-tandem mass spectrometry coupled with a post-column derivatization technique[J].International Journal of Molecular Sciences, 2012, 13(12):260-285.
[29] LOU H X, YAMAZAK Y, SASAKI Y.A-type proanthocyanidins from peanut skins[J].Phytochemistry, 1999, 51(2):297-308.
[30] DUAN L, GUO L, LIU L L, et al.An UHPLC-MS/MS method for simultaneous determination of quercetin 3-O-rutinoside, kaempferol 3-O-rutinoside, isorhamnetin 3-O-rutinoside, bilobalide and ligustrazine in rat plasma, and its application to pharmacokinetic study of Xingxiong injection[J].Chinese Journal of Natural Medicines, 2017, 15(9):710-720.
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