食品与发酵工业 >

2022 , Vol. 48 >Issue 1: 98 - 103

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027693

研究报告

蒲公英根活性组分体外抗菌及抗炎作用

  • 马艳妮 ,
  • 魏悦 ,
  • 李智宁 ,
  • 王志尧 ,
  • 景炳年 ,
  • 宁二娟 ,
  • 杨锐 ,
  • 范毅
展开
  • 1(河南省科学院,河南 郑州,450002)
    2(河南省纳普生物技术有限公司,河南 郑州,450002)
    3(成都理工大学 材料与化学化工学院,四川 成都,610059)
博士,助理研究员(范毅副研究员为通信作者,E-mail:420001839@qq.com)

收稿日期: 2021-04-15

  修回日期: 2021-05-10

  网络出版日期: 2022-01-27

基金资助

河南省科学院人才队伍建设专项(200813002);河南省科学院科研开发专项(210913009)

收起

In vitro antibacterial and anti-inflammatory activity of active components from dandelion root

  • MA Yanni ,
  • WEI Yue ,
  • LI Zhining ,
  • WANG Zhiyao ,
  • JING Bingnian ,
  • NING Erjuan ,
  • YANG Rui ,
  • FAN Yi
Expand
  • 1(Henan Academy of Sciences,Zhengzhou 450002,China)
    2(Henan Natural Product Biotechnology Co.Ltd.,Zhengzhou 450002,China)
    3(College of Materials,Chemistry & Chemical Engineering,Chengdu University of Technology,Chengdu 610059,China)

Received date: 2021-04-15

  Revised date: 2021-05-10

  Online published: 2022-01-27

Fold

摘要

以市售蒲公英根为研究对象,采用乙醇提取、大孔树脂柱分离和活性跟踪相结合的方法优选蒲公英根抗菌抗炎活性组分,通过倍半稀释法测定蒲公英根不同组分对人、畜禽等9种常见病原菌的抑菌作用,通过脂多糖诱导的小鼠巨噬细胞RAW264.7炎症细胞模型评价各组分的抗炎活性。结果表明,蒲公英根抗菌抗炎活性组分的分离工艺为:70%乙醇回流提取后,用D101大孔树脂柱分离,其中,水洗脱之后的70%(体积分数)乙醇洗脱部位为蒲公英根的抗菌抗炎活性部位,即蒲公英根抗菌抗炎活性组分;该组分黄酮含量相对较高,且对9种供试菌具有较强的广谱抗菌性,大多数最小抑菌质量浓度为1.95 mg/mL,最小杀菌质量浓度均为31.25 mg/mL,对脂多糖诱导的小鼠巨噬细胞RAW264.7的炎症因子IL-6、IL-1β和TNF-α的浓度和转录水平均有显著抑制作用(P<0.05)。优选的蒲公英根抗菌抗炎活性组分分离工艺简便、高效,而且具有较强的广谱抗菌作用和良好的抗炎作用。

关键词： 蒲公英根; 含量测定; 活性组分; 抗菌活性; 抗炎活性

本文引用格式

马艳妮 , 魏悦 , 李智宁 , 王志尧 , 景炳年 , 宁二娟 , 杨锐 , 范毅 . 蒲公英根活性组分体外抗菌及抗炎作用[J]. 食品与发酵工业, 2022 , 48(1) : 98 -103 . DOI: 10.13995/j.cnki.11-1802/ts.027693

Abstract

Ethanol extraction, macroporous resin column separation and activity tracking were used for the selection of antibacterial and anti-inflammatory components from dandelion root. The antibacterial and anti-inflammatory activities in vitro against nine common pathogens were determined by the half dilution method, and the anti-inflammatory activity in vitro was assayed by the lipopolysaccharides (LPS)-induced macrophagocyte RAW 264.7. The results showed that the antibacterial and anti-inflammatory active components from dandelion root were separated by D101 macroporous resin column after 70% ethanol reflux extraction. The results showed that 70% ethanol elution fraction, after water elution, presented antibacterial and anti-inflammatory activities. The active components had more flavonoids and broad-spectrum antimicrobial activity against nine tested bacteria (most MIC values were 1.95 mg/mL and all MBC values were 31.25 mg/mL) and the outstanding inhibition of the concentration and transcription levels of inflammatory cytokines IL-6, IL-1β and TNF-α in LPS-induced macrophagocyte RAW 264.7 (P<0.05). The antimicrobial and anti-inflammatory components from the dandelion root have simple and efficient separation technology and broad-spectrum antimicrobial activity and outstanding anti-inflammatory activity.

Key words： dandelion root; content determination; active components; antibacterial activity; anti-inflammatory activity

参考文献

[1] 国家药典委员会.中华人民共和国药典(2020年版 一部)[S].北京:中国医药科技出版社,2020.
Chinese Pharmacopoeia Commision.Pharmacopoeia of the People’s Republic of China(2020,Volume I)[S].Beijing:China Medical Science Press,2020.
[2] 康文锦,徐兴军,刘佳人,等.蒲公英多糖对小鼠体内抗氧化酶活性及相关基因表达的影响[J].动物营养学报,2020,32(12):5 910-5 915.
KANG W J,XU X J,LIU J R,et al.Effects of dandelion polysaccharide on antioxidant enzyme activity and related gene expression in mice[J].Chinese Journal of Animal Nutrition,2020,32(12):5 910-5 915.
[3] 杨超,闫庆梓,唐洁,等.蒲公英挥发油成分分析及其抗炎抗肿瘤活性研究[J].中华中医药杂志,2018,33(7):3 106-3 111.
YANG C,YAN Q Z,TANG J,et al.Study on chemical composition of volatile oil from Taraxaci herba and its antiinflammatory and anti-tumor activities[J].China Journal of Traditional Chinese Medicine and Pharmacy,2018,33(7):3 106-3 111.
[4] REN F,LI J,YUAN X,et al.Dandelion polysaccharides exert anticancer effect on Hepatocellular carcinoma by inhibiting PI3K/AKT/mTOR pathway and enhancing immune response[J].Journal of Functional Foods,2019,55:263-274.
[5] 李超,董自波,蒋金来,等.HPLC法测定蒲公英中菊苣酸、咖啡酸与绿原酸[J].中草药,2015,46(23):3 577-3 580.
LI C,DONG Z B,JIANG J L,et al.Determination of cichoric acid,caffeic acid,and chlorogenic acid in Taraxaci herba by HPLC[J].Chinese Traditional and Herbal Drugs,2015,46(23):3 577-3 580.
[6] 陶亮亮,李鹏.超声辅助提取蒲公英中总黄酮的工艺研究[J].食品与发酵科技,2011,47(4):49-51;67.
TAO L L,LI P.Studying on the extraction process of total flavonoids from Taraxacum by ultrasonic wave[J].Food and Fermentation Technology,2011,47(4):49-51;67.
[7] 李侠,牛秋云,冯建国,等.超声波辅助提取蒲公英中生物碱的工艺研究[J].吉林农业大学学报,2013,35(2):233-235;240.
LI X,NIU Q Y,FENG J G,et al.Study on ultrasound extraction alkaloids from dandelion[J].Journal of Jilin Agricultural University,2013,35(2):233-235;240.
[8] 闫爽,李光耀,戴丛书,等.蒲公英提取物对2型糖尿病大鼠降血糖的作用[J].食品与机械,2020,36(11):138-142.
YAN S,LI G Y,DAI C S,et al.Study on the hypoglycemic effect of dandelion extract on type 2 diabetic rats[J].Food & Machinery,2020,36(11):138-142.
[9] IDDRISU I,ODURO I,TANDO H M A.The effect of dandelion leaves and roots on blood glucose in type 2 diabetic patients[J].Journal of Nutritional Ecology and Food Research,2016,3(2):125-132.
[10] 钟华晨,张三粉,冯小慧,等.植物提取物对奶牛乳房炎致病菌的抑菌效果研究[J].黑龙江畜牧兽医,2020(23):117-121;126.
ZHONG H C,ZHANG S F,FENG X H,et al.Study on the antibacterial effect of plant extracts on the pathogenic bacteria of dairy cow mastitis[J].Heilongjiang Animal Science and Veterinary Medicine,2020(23):117-121;126.
[11] LIANG Y K,DUAN H B,ZHANG P,et al.Extraction and isolation of the active ingredients of dandelion and its antifungal activity against Candida albicans[J].Molecular Medicine Reports,2020,21(1):229-239.
[12] 李欢,许艳艳,高进勇,等.蒲公英根多糖的体外抗炎作用研究[J].动物医学进展,2019,40(5):75-78.
LI H,XU Y Y,GAO J Y,et al.Anti-inflammatory effect of Taraxacum root polysaccharide in vitro[J].Progress in Veterinary Medicine,2019,40(5):75-78.
[13] YANG N,DONG Z B,TIAN G,et al.Protective effects of organic acid component from Taraxacum mongolicum Hand.-Mazz.against LPS-induced inflammation:Regulating the TLR4/IKK/NF-κB signal pathway[J].Journal of Ethnopharmacology,2016,194:395-402.
[14] CHEN J F,WU W B,ZHANG M M,et al.Taraxasterol suppresses inflammation in IL-1β-induced rheumatoid arthritis fibroblast-like synoviocytes and rheumatoid arthritis progression in mice[J].International Immunopharmacology,2019,70:274-283.
[15] KENNY O,J SMYTH T,M HEWAGE C,et al.Antioxidant properties and quantitative UPLC-MS/MS analysis of phenolic compounds in dandelion(Taraxacum officinale) root extracts[J].Free Radicals and Antioxidants,2014,4(1):55-61.
[16] DUAN L,ZHANG C M,ZHAO Y,et al.Comparison of bioactive phenolic compounds and antioxidant activities of different parts of Taraxacum mongolicum[J].Molecules(Basel,Switzerland),2020,25(14):E3260.
[17] 张智慧,邸彦橙,闫本纯,等.蒲公英萜醇对膀胱癌T24细胞增殖和凋亡的影响[J].中国临床药理学杂志,2020,36(17):2 682-2 685.
ZHANG Z H,DI Y C,YAN B C,et al.Effects of taraxerol on proliferation and apoptosis of bladder cancer T24 cells[J].The Chinese Journal of Clinical Pharmacology,2020,36(17):2 682-2 685.
[18] REN F,WU K X,YANG Y,et al.Dandelion polysaccharide exerts anti-angiogenesis effect on hepatocellular carcinoma by regulating VEGF/HIF-1α expression[J].Frontiers in Pharmacology,2020,11:460.
[19] 石丹,张宇.蒲公英多糖对小鼠肠道微生态的调节作用[J].微生物学免疫学进展,2016,44(3):49-53.
SHI D,ZHANG Y.Investigation of regulation from dandelion polysaccharides on mouse intestinal microecology[J].Progress in Microbiology and Immunology,2016,44(3):49-53.
[20] 刘珊珊,刘亚琼.蒲公英根类咖啡产品工艺优化及特性分析[J].食品科技,2018,43(9):134-141.
LIU S S,LIU Y Q.Process optimization and characteristic analysis for coffee-like flavored products of dandelion root[J].Food Science and Technology,2018,43(9):134-141.
[21] 吴海婷,张心媛,崔艳艳.类大麦茶风味蒲公英饮料的研究与开发[J].农产品加工,2019(7):20-23.
WU H T,ZHANG X Y,CUI Y Y.Research and development of dandelion beverage with barley tea flavor[J].Farm Products Processing,2019(7):20-23.
[22] 刘婷,张继秀,李先哲,等.蒲公英的开发与利用[J].沈阳药科大学学报,2019,36(5):450-458.
LIU T,ZHANG J X,LI X Z,et al.The progress on development and utilization of dandelion[J].Journal of Shenyang Pharmaceutical University,2019,36(5):450-458.
[23] 李宁,穆淑琴,闫峻,等.植物提取物和酸制剂替代抗生素对断奶仔猪健康的影响[J].饲料研究,2019,42(11):22-26.
LI N,MU S Q,YAN J,et al.Effect of plant extracts and acid preparations as substitutes for antibiotics on the health of weaned piglets[J].Feed Research,2019,42(11):22-26.
[24] 张静,温暖,刘阳洋,等.蒲公英根多糖提取及蒲公英多糖饮料的研制[J].农产品加工,2018(1):13-17.
ZHANG J,WEN N,LIU Y Y,et al.Extraction of polysaccharide from the dandelion root and development of its beverage[J].Farm Products Processing,2018(1):13-17.
[25] 葛明明,缪月英,孙丽娜,等.蒲公英根多糖的抗氧化活性研究[J].黑龙江医药科学,2014,37(2):39-41.
GE M M,MIAO Y Y,SUN L N,et al.Studies on antioxidant activity of polysaccharides from dandelion root[J].Heilongjiang Medicine and Pharmacy,2014,37(2):39-41.
[26] 马艳妮,王志尧,郭展展,等.忍冬叶总黄酮的测定及其体外抗菌活性[J].中国实验方剂学杂志,2017,23(6):55-59.
MA Y N,WANG Z Y,GUO Z Z,et al.Determination of total flavonoids of Lonicera japonica leaves and their in vitro antibacterial activity[J].Chinese Journal of Experimental Traditional Medical Formulae,2017,23(6):55-59.
[27] 黄传奇,张林碧,章习哲,等.北美圆柏与侧柏挥发性成分体外抑菌活性的比较[J].湖北中医药大学学报,2013,15(2):42-44.
HUANG C Q,ZHANG L B,ZHANG X Z,et al.Vitro antibacterial activity comparasion of volatile components between Sabina virginiana and Platycladus orientalis[J].Journal of Hubei University of Chinese Medicine,2013,15(2):42-44.
[28] 张静,邵永斌,谷新利,等.紫花地丁总黄酮体外抗炎活性研究[J].中国畜牧兽医,2020,47(4):1 258-1 266.
ZHANG J,SHAO Y B,GU X L,et al.Study on anti-inflammatory activity of total flavonoids from Viola yedoensis in vitro[J].China Animal Husbandry & Veterinary Medicine,2020,47(4):1 258-1 266.
[29] 刘文强,张懿玲,熊华,等.基于LPS诱导RAW 264.7巨噬细胞炎症模型的榴莲壳多酚抗炎作用及其分子机制[J].食品与机械,2020,36(4):15-20;50.
LIU W Q,ZHANG Y L,XIONG H,et al.Anti-inflammatory effect and molecular mechanism of durian hull polyphenols on LPS-induced RAW 264.7 macrophages[J].Food & Machinery,2020,36(4):15-20;50.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司