食品与发酵工业 >

2022 , Vol. 48 >Issue 1: 188 - 194

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027639

研究报告

微贮干玉米秸秆和废弃物单相厌氧共消化产沼气能力分析

  • 杨旭 ,
  • 孟祥玉 ,
  • 宋丽丽 ,
  • 张志平 ,
  • 马歌丽 ,
  • 魏涛
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  • 1(郑州轻工业大学 食品与生物工程学院,河南 郑州,450002)
    2(郑州市代谢工程和系统生物学重点实验室,河南 郑州,450002)
博士,讲师(魏涛教授为通信作者,E-mail:weit8008@zzuli.edu.cn)

收稿日期: 2021-04-07

  修回日期: 2021-05-06

  网络出版日期: 2022-01-27

基金资助

郑州轻工业大学博士科研启动基金项目(0123-13501050066);河南省高等学校重点科研项目(20B416003);河南省科技攻关项目(212102110310)

收起

Analysis of biogas production capacity of dry corn stover silage with wastes by single-phase anaerobic co-digestion

  • YANG Xu ,
  • MENG Xiangyu ,
  • SONG Lili ,
  • ZHANG Zhiping ,
  • MA Geli ,
  • WEI Tao
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  • 1(College of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China)
    2(Zhengzhou Key Laboratory of Metabolic Engineering and Systems Biology,Zhengzhou 450002,China)

Received date: 2021-04-07

  Revised date: 2021-05-06

  Online published: 2022-01-27

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摘要

以干玉米秸秆和剩余污泥/猪粪作为发酵原料对预处理方式和厌氧消化工艺进行研究,旨在为现代沼气工程稳定运行提供一定的科学依据。以芽孢杆菌和乳酸菌等微生物进行复配对干玉米秸秆进行微贮预处理,研究秸秆微贮前后的组成变化及微生物多样性,在此基础上考察两相和单相厌氧消化对原料发酵沼气产量的影响,最后采用微贮秸秆和剩余污泥/猪粪进行厌氧共消化以进一步提高沼气产率。结果表明,微贮秸秆pH值降低,有机酸浓度提高,纤维素得到有效保护未被降解;微贮原料中乳酸菌为优势菌群;微贮秸秆两相和单相厌氧消化累积沼气产量分别达到292.06和411.46 mL/g TS;微贮秸秆和剩余污泥/猪粪按照比例混合进行单相厌氧共消化,累积沼气产量分别达到500.97和599.39 mL/g TS,为提高干玉米秸秆和剩余污泥/猪粪混合发酵产气量和发酵效率提供参考。

关键词： 干玉米秸秆; 微贮; 高通量测序; 厌氧共消化; 沼气

本文引用格式

杨旭 , 孟祥玉 , 宋丽丽 , 张志平 , 马歌丽 , 魏涛 . 微贮干玉米秸秆和废弃物单相厌氧共消化产沼气能力分析[J]. 食品与发酵工业, 2022 , 48(1) : 188 -194 . DOI: 10.13995/j.cnki.11-1802/ts.027639

Abstract

In this study, dry corn stover (DCS), excess sludge (ES) and pig manure (PM) were used as fermentation raw materials to study the pretreatment method and anaerobic digestion process in order to provide a scientific basis for the stable operation of modern biogas project. The DCS was pretreated with micro-organisms such as Bacillus and lactic acid bacteria. The composition changes and microbial diversity were studied before and after micro-storage. On this basis, the effects of two-phase and single-phase AD on biogas yield were investigated. Finally, anaerobic co-digestion was carried out to further improve the biogas yield with DCS silage, ES and PM. The results showed that the pH value of DCS silage was reduced, while the concentration of organic acids was increased. Cellulose was protected effectively without degradation. The lactic acid bacteria were the dominant bacteria in the DCS silage. The cumulative biogas yield of DCS by two-phase AD and DCS silage single-phase AD reached 292.06 and 411.46 mL/g TS, respectively. The DCS silage and ES/PM were mixed in proportion for single-phase anaerobic co-digestion, the cumulative biogas production reached 500.97 and 599.39 mL/g TS, respectively. These results provided a reference for improving the gas production and fermentation efficiency of the mixed materials.

Key words： dry corn stover; silage; high throughput sequencing technology; anaerobic digestion; biogas

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