中华块菌是我国重要的商业块菌之一,攀枝花和会东地区是中华块菌的主要产地,为探究攀枝花与会东地区中华块菌的组成成分及差异性,利用气相色谱质谱联用仪(GC-MS)技术对这2个地区新鲜中华块菌成分进行检测和分析。主成分分析表明两地中华块菌组成成分差异较小,两地中华块菌均鉴定出83种有效化合物,包括:15种氨基酸(其中8种为必需氨基酸)、10种糖类、22种有机酸等。偏最小二乘法分析表明两地中华块菌在肌醇半乳糖苷、尿苷、亚油酸、9-(E)-十八烯酸、花生四烯酸等物质含量上有显著性差异。该研究通过分析不同地区中华块菌的组成成分及含量差异,探究中华块菌成分组成的同时还能辅助区分中华块菌的产地,弥补了传统的真菌内转录间隔区鉴定法不能对中华块菌进行产地区分的缺点。
Tuber sinense is one of the most important truffles in commercial, Panzhihua and Huidong are two main producing regions of T. sinense. The truffles from Panzhihua and Huidong were analyzed by GC-MS. The results of PCA indicated that the composition difference between the two truffles were not significant. The truffles, from both regions, contained 83 of effective compounds, 15 of amino acids (8 of which are essential ones), 10 of saccharides, and 22 of organic acids. The results of PLS-DA indicated a significant difference in the content of galactinol, uridine, octadecadien, 9-(E)-octadece, and arabinonic acid were detected. These results mitigated the limitations of the traditional ITS identification method that cannot distinguish the producing area of Tuber sinense.
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