食品与发酵工业 >

2022 , Vol. 48 >Issue 2: 8 - 14

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027307

研究报告

重组谷氨酸棒杆菌全细胞催化一步法合成高果糖浆

  • HABIMANA ,
  • 乔郅钠 ,
  • 徐美娟 ,
  • 杨套伟 ,
  • 张显 ,
  • 邵明龙 ,
  • 饶志明
展开
  • (江南大学 生物工程学院,江苏 无锡,214122)
硕士研究生(饶志明教授为通信作者,E-mail:raozhm@jiangnan.edu.cn)

收稿日期: 2021-03-10

  修回日期: 2021-05-05

  网络出版日期: 2022-02-28

基金资助

国家自然科学基金项目(31770058);江苏省自然科学基金项目(BK20181205)

收起

Recombinant Corynebacterium glutamicum whole cell catalyzes one-step synthesis of high fructose syrup

  • HABIMANA ,
  • QIAO Zhina ,
  • XU Meijuan ,
  • YANG Taowei ,
  • ZHANG Xian ,
  • SHAO Minglong ,
  • RAO Zhiming
Expand
  • (School of Biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2021-03-10

  Revised date: 2021-05-05

  Online published: 2022-02-28

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摘要

高果糖浆是一种可替代蔗糖的甜味剂,在食品和饮料行业应用广泛。该研究实现了经密码子优化的密苏里游动放线菌(Actinoplanes missouriensis) CICIM B0118(A)来源的葡萄糖异构酶在食品安全菌株谷氨酸棒杆菌(Corynebacterium glutamicum) 13032中的异源表达,构建了重组谷氨酸棒杆菌C.glutamicum 13032/pXMJ19-xylA。以其细胞菌体作为全细胞催化剂,对D-葡萄糖异构化生成D-果糖的全细胞转化体系进行优化,结果表明,菌体质量浓度40 g/L、底物D-葡萄糖质量浓度180 g/L、Mg2+浓度10 mmol/L、Co2+浓度1 mmol/L,70 ℃、220 r/min下连续转化18 h,D-果糖质量浓度达到103.32 g/L,转化率为57.4%,实现了高果糖浆的一步法安全生物合成。该研究为高果糖浆的工业化生产提供了实验和理论基础,具有重要的参考价值。

关键词： 葡萄糖异构酶; 异构化; 全细胞催化; 谷氨酸棒杆菌; 高果糖浆

本文引用格式

HABIMANA , 乔郅钠 , 徐美娟 , 杨套伟 , 张显 , 邵明龙 , 饶志明 . 重组谷氨酸棒杆菌全细胞催化一步法合成高果糖浆[J]. 食品与发酵工业, 2022 , 48(2) : 8 -14 . DOI: 10.13995/j.cnki.11-1802/ts.027307

Abstract

High fructose syrup is a sweetener that can replace sucrose, which is widely applied in the food and beverage industries. In this study, the codon-optimized glucose isomerase from Actinoplanes missouriensis CICIM B0118 (A) was expressed heterologously in the food-safe strain Corynebacterium glutamicum 13032, and the recombinant C. glutamicum 13032/pXMJ19-xylA was constructed. The whole cell conversion system of D-glucose isomerization to the biosynthesis of D-fructose was optimized. The results showed that the yield of D-fructose reached 103.32 g/L when the continuous conversion was performed at 70 ℃ and 220 r/min for 18 h, with 40 g/L bacterial cells, 180 g/L D-glucose, 10 mmol/L Mg2+ , and 1 mmol/L Co2+ . The conversion rate was 57.4%, and the safe one-step biosynthesis of high fructose syrup was achieved. This research provided an experimental and theoretical basis for the industrialized synthesis of high fructose syrup, and had important reference value.

Key words： glucose isomerase; isomerization; whole cell catalysis; Corynebacterium glutamicum; high fructose syrup

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