食品与发酵工业 >

2022 , Vol. 48 >Issue 2: 139 - 143

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027539

研究报告

两种产地黄小米多酚的提取及其对神经细胞氧化损伤的保护作用

  • 梁婷 ,
  • 陆奕成 ,
  • 刘彤 ,
  • 管彤 ,
  • 李森
展开
  • (上海理工大学 医疗器械与食品学院,上海,200093)
硕士研究生(李森副教授为通信作者,E-mail:lisen_1027@126.com)

收稿日期: 2021-04-04

  修回日期: 2021-04-13

  网络出版日期: 2022-02-28

基金资助

国家自然科学基金项目(31901609);2020年上海理工大学大学生创新创业训练项目(XJ2020297)

收起

Polyphenols extraction from two kinds of origins of millet yellow and their protective effects on oxidative injury on nerve cells

  • LIANG Ting ,
  • LU Yicheng ,
  • LIU Tong ,
  • GUAN Tong ,
  • LI Sen
Expand
  • (School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)

Received date: 2021-04-04

  Revised date: 2021-04-13

  Online published: 2022-02-28

Fold

摘要

该研究选取具有代表性的山东金乡金小米和河北蔚县桃花小米,比较二者多酚的含量及种类,并通过建立过氧化氢(H2O2)诱导的SH-SY5Y氧化应激损伤的神经细胞模型,探究不同处理方式下小米多酚对神经细胞氧化损伤的保护作用及最佳作用浓度。结果表明,桃花小米多酚含量更高,金小米中多酚种类较多,小米多酚中黄酮类化合物占主要成分。预处理的方式下200~300 μg/mL 2种小米多酚都可以保护SH-SY5Y免受H2O2的氧化损伤。

关键词： 小米多酚; 高效液相色谱; 黄酮; 氧化应激; 神经保护

本文引用格式

梁婷 , 陆奕成 , 刘彤 , 管彤 , 李森 . 两种产地黄小米多酚的提取及其对神经细胞氧化损伤的保护作用[J]. 食品与发酵工业, 2022 , 48(2) : 139 -143 . DOI: 10.13995/j.cnki.11-1802/ts.027539

Abstract

The representative millet jinxiang golden millet in Shandong province and millet peach blossom in Yuxian county, Hebei province were used as raw materials to compare the content and types of polyphenols in them. And the oxidative stress damage model of SH-SY5Y induced by hydrogen peroxide (H2O2) was established to explore the protective effect of millet polyphenols and the optimal concentration under different treatments. The results showed that the content of polyphenols in Taohua millet was higher than that in golden millet, and the polyphenols in millet were mainly composed of flavonoids. Both two millet polyphenols could protect SH-SY5Y from the oxidative damage of H2O2 when the cells were pretreated with a concentration of 200-300 μg/mL.

Key words： millet polyphenol; HPLC; flavonoids; oxidative stress; nerve protection

参考文献

[1] SKOVRONSKY D M, LEE V M Y, TROJANOWSKI J Q.Neurodegenerative diseases:New concepts of pathogenesis and their therapeutic implications[J].Annual Review of Pathology:Mechanisms of Disese, 2006, 1:151-170.
[2] CHEIGNON C, TOMAS M, BONNEFONT-ROUSSELOT D, et al.Oxidative stress and the amyloid beta peptide in Alzheimer's disease[J].Redox Biology, 2018, 14:450-464.
[3] LIN M T, BEAL M F.Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases[J].Nature, 2006, 443(7 113):787-795.
[4] SILVA R F M, POGAČNIK L.Polyphenols from food and natural products:Neuroprotection and safety[J].Antioxidants (Basel), 2020, 9(1):61.
[5] ABIB R T, PERES K C, BARBOSA A M, et al.Epigallocatechin-3-gallate protects rat brain mitochondria against cadmium-induced damage[J].Food Chemical Toxicology, 2011, 49(10):2 618-2 623.
[6] EL-MISSIRY M A, OTHMAN A I, EL-SAWY M R, et al.Neuroprotective effect of epigallocatechin-3-gallate (EGCG) on radiation-induced damage and apoptosis in the rat Hippocampus[J].International Journal of Radiation Biology, 2018, 94(9):798-808.
[7] CHUNG W G, MIRANDA C L, MAIER C S.Epigallocatechin gallate (EGCG) potentiates the cytotoxicity of rotenone in neuroblastoma SH-SY5Y cells[J].Brain Research, 2007, 1 176:133-142.
[8] LI S, XIAN F R, GUAN X, et al.Neural protective effects of millet and millet polyphenols on high-fat diet-induced oxidative stress in the brain[J].Plant Foods for Human Nutrition, 2020, 75(2):208-214.
[9] DYKES L, ROONEY L W.Sorghum and millet phenols and antioxidants[J].Journal of Cereal Science, 2006, 44(3):236-251.
[10] 田志琴. 小米多酚类活性物质的提取及抗氧化性研究[D].郑州:河南工业大学, 2011.
TIAN Z Q.The studies on extraction and antioxidant activities of foxtail millet ployphenol[D].Zhengzhou:He'nan University of Technology, 2011.
[11] 延莎, 祁鹏煜, 张苏慧,等.不同米色小米多酚提取物的体外抗氧化活性[J].中国粮油学报, 2017, 32(10):33-38;44.
YAN S, QI P Y, ZHANG S H, et al.Antioxidant activity of polyphenol extracts from different beige millet in vitro[J].Journal of the Chinese Cereals and Oils Association, 2017, 32(10):33-38;44.
[12] DEWANTO V, WU X Z, LIU R H.Processed sweet corn has higher antioxidant activity[J].Journal of Agricultural and Food Chemistry, 2002, 50(17):4 959-4 964.
[13] BOX J D.Investigation of the folin-ciocalteau phenol reagent for the determination of polyphenolic substances in natural waters[J].Water Research, 1983, 17(5):511-525.
[14] ZHA H H, CAI Y P, YIN Y D, et al.SWATH to MRM:Development of high-coverage targeted metabolomics method using SWATH technology for biomarker discovery[J].Analytical Chemistry, 2018, 90(6):4 062-4 070.
[15] LUO P, YIN P Y, ZHANG W J, et al.Optimization of large-scale pseudotargeted metabolomics method based on liquid chromatography-mass spectrometry[J].Journal of Chromatography A, 2016, 1 437:127-136.
[16] XIANG J L, APEA-BAH F B, NDOLO V U, et al.Profile of phenolic compounds and antioxidant activity of finger millet varieties[J].Food Chemistry, 2019, 275:361-368.
[17] 朱俊玲, 梁凯, 闫巧珍,等.小米多酚的分离纯化及其组分分析[J].中国粮油学报 2021, 36(2):34-40.
ZHU J L, LIANG K, YAN Q Z, et al.Isolation, purification and component analysis of polyphenols from millet [J].Journal of the Chinese of Cereals and Oils Association, 2021, 36(2):34-40.
[18] PENG Y, GAN R Y, LI H B, et al.Absorption, metabolism, and bioactivity of vitexin:Recent advances in understanding the efficacy of an important nutraceutical[J].Critical Reviews in Food Science and Nutrition, 2021, 61(6):1 049-1 064.
[19] YAHAYA M A F, ZOLKIFFLY S Z I, MOKLAS M A M, et al.Possible epigenetic role of vitexin in regulating neuroinflammation in alzheimer's disease[J].Journal of Immunology Research, 2020:9469210.
[20] LEI X B, YANG Y.Vitexin and an HMG-Co a reductase inhibitor prevent the risks of atherosclerosis in high-fat atherogenic diet fed rats[J].Journal of King Saud University - Science, 2020, 32(3):2 088-2 095.
[21] ZHANG G N, LI D Y, CHEN H, et al.Vitexin induces G2/M-phase arrest and apoptosis via Akt/mTOR signaling pathway in human glioblastoma cells[J].Molecular Medicine Reports, 2018, 17(3):4 599-4 604.
[22] 艾凤伟, 佟雷, 杨开勇,等.甲基-β-环糊精对香叶木苷的增溶作用[J].中国实验方剂学杂志 2012, 18(7):41-43.
AI F W, TONG L, YANG K Y, et al.Solubilization effect of methyl-β-cyclodextrin on diosmin[J].Chinese Journal of Experimental Formulae, 2012, 18(7):41-43.
[23] CHEUNG Y T, LAU W K W, YU M S, et al.Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma as in vitro model in neurotoxicity research[J].Neuro Toxicology, 2009, 30(1):127-135.
[24] 吴枝娟, 余靖, 王瑞幸, 等.阿魏酸预处理对阿霉素诱导H9c2心肌细胞损伤的保护作用[J].中国药理学通报 2014,30(8):1 059-1 065.
WU Z J, YU J, WANG R X, et al.Protective effect of ferulic acid on doxorubicin induced cellular injury in H9c2 myocardial cells[J].Chinese Pharmacological Bulletin 2014,30(8):1 059-1 065.
[25] AGATI G, AZZARELLO E, POLLASTRI S, et al.Flavonoids as antioxidants in plants:Location and functional significance[J].Plant Science, 2012, 196:67-76.
[26] HU M, LI F M, WANG W D.Vitexin protects dopaminergic neurons in MPTP-induced Parkinson's disease through PI3K/Akt signaling pathway[J].Drug Design Development and Therapy, 2018, 12:565-573.
[27] ZHAO H F, WANG Q, CHENG X J, et al.Inhibitive effect of resveratrol on the inflammation in cultured astrocytes and microglia induced by Abeta1-42[J].Neuroscience, 2018, 379:390-404.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司