食品与发酵工业 >

2022 , Vol. 48 >Issue 2: 156 - 162

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027445

研究报告

嗜黏蛋白阿克曼氏菌ATCC BAA-835肠道益生作用的体外评价

  • 吴艳丽 ,
  • 刘朋 ,
  • 苏咏欣 ,
  • 李恒 ,
  • 耿燕 ,
  • 任怡琳 ,
  • 许泓瑜 ,
  • 许正宏 ,
  • 史劲松
展开
  • 1(江南大学 生命科学与健康工程学院,江苏 无锡,214122)
    2(糖化学与生物技术教育部重点实验室,江苏 无锡,214122)
    3(粮食发酵与食品生物制造国家工程研究中心,江苏 无锡,214122)
    4(江南大学 生物工程学院,江苏 无锡,214122)
硕士研究生(李恒副教授和史劲松教授为共同通信作者,E-mail:liheng@jiangnan.edu.cn;shijs@163.com)

收稿日期: 2021-03-24

  修回日期: 2021-04-16

  网络出版日期: 2022-02-28

基金资助

江苏省重点研发项目(BE2018622);宁夏回族自治区重点研发项目(2020BFH02011);宁夏回族自治区特色农产品生物加工创新团队项目(kjt201701);江苏高校境外研究计划

收起

Intestinal probiotic effect of Akkermansia muciniphila ATCC BAA-835 in vitro

  • WU Yanli ,
  • LIU Peng ,
  • SU Yongxin ,
  • LI Heng ,
  • GENG Yan ,
  • REN Yilin ,
  • XU Hongyu ,
  • XU Zhenghong ,
  • SHI Jinsong
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  • 1(School of Life Science and Health Engineering, Jiangnan University, Wuxi 214122, China)
    2(Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Wuxi 214122, China)
    3(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Wuxi 214122, China)
    4(School of Biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2021-03-24

  Revised date: 2021-04-16

  Online published: 2022-02-28

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摘要

嗜黏蛋白阿克曼氏菌(Akkermansia muciniphila,Akk)具有良好的肠道益生作用,与宿主肠道健康、代谢、神经等生理状态密切相关。研究采用体外厌氧培养的方法评价1株Akk模式菌株(ATCC BAA-835)的肠道益生作用,从菌株水平考查菌株对模拟胃肠环境的耐受性、对Caco-2细胞的黏附性,以及利用低聚糖产短链脂肪酸和形成生物膜的能力。结果表明,Akk对人工胃肠液和胆盐的耐受性良好,能够黏附在分化的Caco-2细胞微绒毛尖端上。Akk在低聚糖培养基中生长良好,培养12 h内快速消耗还原糖并产生短链脂肪酸,乙酸产量最高,其次是丁酸。Akk自身具有弱生物膜形成能力,低聚糖可显著提高其成膜能力,在壳寡糖质量浓度为250 μg/mL时,Akk具有中等生物膜形成能力。该研究借助微生物纯培养与细胞模型等体外研究方法,进一步了解了Akk发挥肠道益生作用的微生物学基础。

关键词： 嗜黏蛋白阿克曼氏菌; 纯培养; 体外评价; 低聚糖; 益生作用

本文引用格式

吴艳丽 , 刘朋 , 苏咏欣 , 李恒 , 耿燕 , 任怡琳 , 许泓瑜 , 许正宏 , 史劲松 . 嗜黏蛋白阿克曼氏菌ATCC BAA-835肠道益生作用的体外评价[J]. 食品与发酵工业, 2022 , 48(2) : 156 -162 . DOI: 10.13995/j.cnki.11-1802/ts.027445

Abstract

Akkermansia muciniphila (Akk) possesses superior intestinal probiotic effect which is closely related to the host's physiological conditions, including intestinal, metabolic and neurological health. In this study, the intestinal probiotic effect of an Akk model strain (ATCC BAA-835) was evaluated using anaerobic cultivation method in vitro. The probiotic abilities of Akk were investigated, including the tolerance to simulated gastrointestinal environment, adhesion to Caco-2 cells, production of short-chain fatty acids and formation of biofilms utilizing oligosaccharides. The results showed that this Akk stain performed good tolerance to artificial gastrointestinal fluids and bile salts. It could adhere to the microvilli tip of differentiated Caco-2 cells. This Akk strain grew well in the medium with various oligosaccharides. It could quickly consume reducing sugars within 12 h and produce short-chain fatty acids, of which the output of acetic acid was the highest, followed by butyric acid. Akk possessed weak biofilm-forming ability, which could be improved significantly with the addition of oligosaccharides. And Akk could form biofilms at a medium level when the concentration of chito-oligosaccharides was 250 μg/mL. This study could provide further understanding for the microbiological basis of Akk's intestinal probiotic effect with assessments in vitro including pure microbial culture and cell models.

Key words： Akkermansia muciniphila; pure culture; assessment in vitro; oligosaccharides; probiotic effect

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