为探究复合益生菌剂量与动物肠道菌群及生理代谢之间的关系,选取6周龄BALB/C小鼠,每天按108(低)、109(中)、1010 CFU/mL(高)分别灌胃,持续6周,测定小鼠体重增加量、血清生化指标、脏器结构以及肠道菌群。结果表明,中、低剂量组小鼠的体重增加量和饲料利用率显著高于对照组,且能够增强小鼠肠道微生物的代谢功能,而血清总蛋白、白蛋白与对照组差异不显著。但高剂量组小鼠的血清指标、肠道和肝肾脏器官结构发生变化。其中,高剂量组血清中丙氨酸氨基转移酶和天门冬氨酸氨基转移酶活性分别为69.8±5.2和52.7±3.1,显著高于对照组和中、低剂量组;肝脏组织周围有轻微纤维化,肾小球有轻微萎缩,表明高剂量组小鼠肝肾细胞受损;肠道菌群结构分析表明,高剂量组的厚壁菌门减少,拟杆菌门、变形菌门增加,且代谢功能丰度降低。研究表明,适量补充益生菌对机体健康具有一定促进作用,但剂量过高可能会带来潜在的健康风险。
To explore the relationship between the dose of compound probiotics and intestinal microflora, growth performance, metabolism of experimental animal, 6-week-old BALB/C mice were intragastric administration with 108 (low dose), 109 (medium dose) and 1010 CFU/mL (high dose) compound probiotics every day for 6 weeks. The weight gain, serum biochemical indexes, organ structure and intestinal microflora were assessed. The results showed that the weight gain and feed utilization rate of mice in medium and low dose group were significantly higher than that of control, and the metabolic function of intestinal microorganisms in mice was enhanced. There was no significant difference of serum total protein and albumin. However, changes in the structure of intestine, liver and kidney in high dose groups were observed. The activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were (69.8±5.2) and (52.7±3.1) in high dose group, which was significantly higher than those in other groups. There was slight fibrosis around liver tissue and slight atrophy of glomerulus, which indicated that damage of liver and kidney cells were induced by high dose compound probiotics intragastric administration. The intestinal flora structure analysis suggested that the Firmicutes decreased, the Bacteroides and Proteus increased, and the metabolic function abundance decreased in high dose group. Therefore, supplement probiotics properly can promote health of body. Excessive doses may pose a risk to the health of human and animal.
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