乳铁蛋白(lactoferrin,Lf)是一种对婴幼儿有重要生理功能的活性乳蛋白,掌握Lf特异性分离技术对打破国外技术垄断、降低成本、促进我国婴幼儿配方奶粉发展具有重要意义。该研究以乳清为原料,基于最大吸附量、吸附率、特异吸附为指标,对不同吸附剂做了筛选,探讨了工艺参数对吸附效果的影响及洗脱液离子浓度对洗脱效果的影响,研究了阳离子交换树脂X从乳清中静态吸附分离Lf工艺条件。结果表明,最佳的吸附条件为pH 5.17、搅拌转速为300 r/min、树脂与乳清的体积比为1∶15、作用时间为3 h;最佳的洗脱条件为0.3 mol/L NaCl+0.02 mol/L磷酸盐缓冲液(phosphate buffer saline,PBS)、蒸馏水、0.9 mol/L NaCl +0.02 mol/L PBS顺次洗脱,乳清经过上述工艺处理获得Lf的蛋白纯度为95.5%,回收率为55.3%,获得的Lf纯度符合国家标准,吸附剂成本较低,具备工业化分离Lf的潜力。
杜健
,
张光艳
,
董博
,
王瑞琦
,
刘银雪
,
刘同杰
,
易华西
,
公丕民
,
张兰威
. 阳离子交换树脂静态吸附法特异性分离乳清中乳铁蛋白的关键技术[J]. 食品与发酵工业, 2022
, 48(3)
: 99
-106
.
DOI: 10.13995/j.cnki.11-1802/ts.028588
Lactoferrin (Lf) is an active milk protein, which has important physiological functions for infants. Mastering the specific separation technology of Lf is of significantly important to break the foreign technology monopoly, reduce the cost and promote the development of infant formula in China. In this study, whey was used as raw material to explore the influence of process parameters on the adsorption effect and the influence of ion concentration of eluent on the elution effect by using the maximum adsorption capacity, adsorption yield, and specific adsorption as indicators. Different adsorbents were also been screened. And the process conditions for the separation of Lf from whey by cation exchange resin X by static adsorption were studied. The results showed that the optimum adsorption conditions were as follows: reacted at pH 5.17 for 3h with the rotating at 300 r/min with the volume ratio of resin to whey equaled to 1∶15. The best elution conditions were as follows: 0.3 mol/L NaCl+0.02 mol/L phosphate buffer saline (PBS), distilled water, 0.9 mol/L NaCl+0.02 mol/L PBS eluted sequentially. The protein purity of Lf obtained was 95.5%, and the recovery rate was 55.3%. The purity of the obtained Lf meets the standards of the nation, the adsorbent cost is low, and it has the potential for industrial separation of Lf.
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