响应面法优化合浦珠母贝肉水解工艺

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  • 1 (中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,国家水产品加工技术研发中心,广东广州 510300) 2(上海海洋大学 食品学院 , 上海, 201306)
王安凤(1991-),女,硕士研究生,研究方向为水产品精深加工与利用,E-mail:anfengw@126.com。

网络出版日期: 2017-11-30

基金资助

广东省海洋渔业科技与产业发展专项(Z2014001,Z2015007,Z2015008,A201501C08);广东省公益研究与能力建设专项(2014A020217009);广东省自然科学基金项目(2016A030313144);中央级公益性科研院所基本科研业务费专项资金项目(2014TS25)。

Optimization of hydrolysis process of pinctadafucata by response surface method

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  • (1.Key Laboratory of Aquatic Product Processing, Ministry of Agriculture; National R&D Center for Aquatic Product Processing; South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China) (2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)

Online published: 2017-11-30

摘要

采用响应曲面法对合浦珠母贝肉的酶解工艺进行优化。以水解度为评价指标,分别考察加酶量、液料比、酶解时间和酶解温度对水解效果的影响。结果表明:贝肉最佳水解条件为加酶量5680 U/g、液料比2.8:1(Vm)、酶解时间6.5 h、酶解温度55 ℃,在此条件下水解度为44.27%±1.23%,与预测值基本吻合。该工艺简单方便,水解效果好,可用于指导生产实践。

本文引用格式

王安凤, 赵永强, 陈胜军, 等 . 响应面法优化合浦珠母贝肉水解工艺[J]. 食品与发酵工业, 2017 , 43(11) : 165 . DOI: 10.13995/j.cnki.11-1802/ts.014525

Abstract

Response surface method was used to optimize the hydroiysis conditions of Pinctadafucata. The effects of the dose of protease, liquid-material ratio, enzymolysis time and enzymolysis temperature were investigated by degree of hydrolysis. The results showed that the optimized conditions were as follows : 5680 U/g of the amount of enzyme, liquid-material ratio 2.8:1(Vm), 6.5 h ofenzymolysis time, 55 ℃ of the enzymolysis temperature. Under this optimized conditions, the degree of hydrolysis was 44.27±1.23% which was almost same with the theoretical value. The hydrolysis process was simple, reliable, high hydrolysis rate and could beapply to practical production .
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