食品与发酵工业 >

2022 , Vol. 48 >Issue 5: 187 - 192

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029212

研究报告

不同脱腥方法对海参肠卵酶解液脱腥效果的比较

  • 陈增鑫 ,
  • 刘咏霖 ,
  • 潘芸芸 ,
  • 姜竹茂 ,
  • 洪杏德
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  • 1(烟台大学 生命科学学院,山东 烟台,264005)
    2(山东承锦海洋生物科技有限公司,山东 烟台,264000)
硕士研究生(姜竹茂副教授为通信作者,E-mail:271277394@qq.com)

网络出版日期: 2022-04-06

基金资助

山东省自然科学基金重点项目(ZR2020KC013);烟台市科技创新发展计划项目(2020XDRH110)

收起

Effect of different deodorization methods on enzymatic hydrolysate of sea cucumber (Stichopus japonicas) intestines and gonads

  • CHEN Zengxin ,
  • LIU Yonglin ,
  • PAN Yunyun ,
  • JIANG Zhumao ,
  • HONG Xingde
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  • 1(College of Life Science,Yantai University,Yantai 264005,China)
    2(Shandong Chengjin Marine Biotechnology Co.Ltd.,Yantai 264000,China)

Online published: 2022-04-06

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摘要

为提高海参副产物的综合利用率,以海参肠卵酶解液为原料,采用生姜掩盖法、活性炭吸附法、大孔树脂吸附法、酵母发酵法和乳酸菌发酵法进行脱腥处理,以感官值协同电子舌、电子鼻和顶空固相微萃取气相色谱-质谱联用技术(headspace-solid phase micro-extraction/GC-MS,HS-SPME/GC-MS)数据为评定依据进行脱腥效果分析,择出较优的脱腥方法。结果表明,生姜掩盖法的腥味值较高,且有较重生姜味;活性炭和大孔树脂吸附法脱腥效果一般;酵母法脱腥易引入菌体异味,影响脱腥效果;乳酸菌发酵法的脱腥效果较好,最适宜的条件为乳酸菌添加量0.4%、40 ℃、10 h,腥味值较低,且庚醛、反,顺-2,6-壬二烯醛等腥味物质显著降低,刺激性气味减少。综合分析可得,乳酸菌发酵法更适用于海参肠卵酶解液的脱腥。

关键词： 脱腥; 海参肠卵酶解液; 电子鼻; 电子舌; 顶空固相微萃取/气相色谱-质谱联用技术(HS-SPME/GC-MS)

本文引用格式

陈增鑫 , 刘咏霖 , 潘芸芸 , 姜竹茂 , 洪杏德 . 不同脱腥方法对海参肠卵酶解液脱腥效果的比较[J]. 食品与发酵工业, 2022 , 48(5) : 187 -192 . DOI: 10.13995/j.cnki.11-1802/ts.029212

Abstract

In order to improve the comprehensive utilization rate of the by-products from sea cucumber, sea cucumber (Stichopus japonicas) intestine and gonads enzymatic hydrolysate was used as raw material. The ginger masking, active carbon adsorption, macroporous resin adsorption, yeast fermentation and lactic acid bacteria fermentation method were used for deodorization. The deodorization effect was analyzed based on the sensory value, the date of electronic tongue, electronic nose and headspace-solid phase micro-extraction/gas chromatography-mass spectrometry, and the optimal deodorization method was selected. The results showed that the ginger masking method had higher odor value with heavier ginger flavor. Deodorization effect of activated carbon and macroporous resin adsorption method was general. The yeast deodorization could easily introduce bacteria odor, which had side effect on deodorization. The deodorization effect of lactic acid bacteria fermentation was better. The optimum conditions were 0.4% lactic acid bacteria, 40 ℃, 10 h. The odor value was lower. The odor substances and the irritant odor decreased significantly, such as heptanal and (E,Z)-2,6-nonadienal. Comprehensive analysis showed that lactic acid bacteria fermentation was more suitable for deodorization of sea cucumber intestinal and gonads enzymatic hydrolysate.

Key words： deodorization; sea cucumber (Stichopus japonicas) intestine and gonads enzymatic hydrolysate; electronic nose; electronic tongue; headspace-solid phase micro-extraction/GC-MS (HS-SPME/GC-MS)

参考文献

[1] BORDBAR S,ANWAR F,SAARI N.High-value components and bioactives from sea cucumbers for functional foods-a review[J].Marine Drugs,2011,9(10):1 761-1 805.
[2] DONG X F,QI H,FENG D D,et al.Oxidative stress involved in textural changes of sea cucumber Stichopus japonicus body wall during low-temperature treatment[J].International Journal of Food Properties,2018,21(1):2 646-2 659.
[3] 杨兰苹,郑立勇,饶煜,等.海参内脏的活性成分、保健功能与开发研究进展[J].食品安全质量检测学报,2018,9(10):2 426-2 432.
YANG L P,ZHENG L Y,RAO Y,et al.Research progress of active components,health function and development of sea cucumber viscera[J].Journal of Food Safety& Quality,2018,9(10):2 426-2 432.
[4] 赵晓玥.海参肠、卵酶解物的制备工艺及性质研究[D].大连:大连海洋大学,2016.
ZHAO X Y.Study on preparation and properties of seacucumber(Stichopus japonicas) intestines and gonads enzymatic hydrolyate[D].Dalian:Dalian Ocean University,2016.
[5] YAN M Y,TAO H T,QIN S.Effect of enzyme type on the antioxidant activities and functional properties of enzymatic hydrolysates from sea cucumber(Cucumaria frondosa) viscera[J].Journal of Aquatic Food Product Technology,2016,25(6):940-952.
[6] LIANG S Q,ZHANG T,FU X D,et al.Partially degraded chitosan-based flocculation to achieve effective deodorization of oyster(Crassostrea gigas) hydrolysates[J].Carbohydrate Polymers,2020,234:115948.
[7] ZHANG Y,HE S D,BONNEIL,et al.Generation of antioxidative peptides from Atlantic sea cucumber using alcalase versus trypsin:In vitro activity,de novo sequencing,and in silico docking for in vivo function prediction[J].Food Chemistry,2020,306:125581.
[8] 毛毛,侯虎,郭红,等.脂肪酶在海参内脏多肽气味与口感可接受性优化中的应用[J].食品工业科技,2019,40(13):94-99.
MAO M,HOU H,GUO H,et al.Application of lipase on smell and mouth feel modification of sea cucumber viscera peptide[J].Science and Technology of Food Industry,2019,40(13):94-99.
[9] 洪伟,周春霞,洪鹏志,等.水产品腥味物质的形成及脱腥技术的研究进展[J].食品工业科技,2013,34(8):386-389;399.
HONG W,ZHOU C X,HONG P Z,et al.Research progress in the formation and deodorization technology of fishy odor for aquatic product[J].Science and Technology of Food Industry,2013,34(8):386-389;399.
[10] 王文勇,张英慧,赖长生.水产品腥味物质去除及提取分析的研究进展[J].肉类工业,2017(3):50-53.
WANG W Y,ZHANG Y H,LAI C S.Research progress on removing and extraction and analysis of fishy odor materials in aquatic products[J].Meat Industry,2017(3):50-53.
[11] 苏怡,姜启兴,夏文水.不同脱腥方法对鲟鱼肉脱腥效果的比较研究[J].食品科技,2019,44(10):138-146.
SU Y,JIANG Q X,XIA W S.Comparison of effects of different deodorization methods on deodorizing sturgeon fish(Acipenser sinensis)[J].Food Science and Technology,2019,44(10):138-146.
[12] 付湘晋.白鲢鱼脱腥及其低盐鱼糜制备的研究[D].无锡:江南大学,2009.
FU X J.Removing the off-flavor of silver carp and the preparation of low salt surimi[D].Wuxi:Jiangnan University,2009.
[13] 王小康,林绮莲,黎颖,等.罗非鱼皮胶原蛋白中腥味物质的鉴定及不同脱腥方法的脱腥效果比较[J].食品科技,2016,41(5):234-239.
WANG X K,LIN Q L,LI Y,et al.Identifi cation and elimination of odorous compounds from Tilapia skin collagen[J].Food Science and Technology,2016,41(5):234-239.
[14] ESHTIAGHI M N,KULDILOKE J,YOSWATHANA N.Deodorization of coconut oil using activated charcoal and charcoal regeneration[J].Journal of Food,Agriculture & Environment,2012,10(3-4):178-181.
[15] 王晓君,夏杨毅,张丹,等.不同脱腥方法对兔肉脱腥效果的比较[J].现代食品科技,2016,32(5):205-212;179.
WANG X J,XIA Y Y,ZHANG D,et al.Comparison of different deodorization methods for deodorizing rabbit meat[J].Modern Food Science and Technology,2016,32(5):205-212;179.
[16] 赵彦珺.基于气相离子迁移谱研究酶解及发酵对海参肠卵挥发性物质的影响[D].烟台:烟台大学,2020.
ZHAO Y J.The effect ofenzymolysis and fermentation on the volatile compounds in the intestines and gonads of sea cucumber using headspace-gas chromatography-ion mobility spectroscopy(HS-GC-IMS)[D].Yantai:Yantai University,2020.
[17] 梁杰,赵晓旭,杨志强,等.鲍鱼内脏酶解液抗氧化能力及脱腥工艺研究[J].食品研究与开发,2019,40(2):112-119.
LIANG J,ZHAO X X,YANG Z Q,et al.Study on antioxidant ability and deodorization process of abalone visceral enzymatic hydrolysate[J].Food Research and Development,2019,40(2):112-119.
[18] 闫泽文,郑思凡,姜竹茂,等.一种海参肠卵酵素及其制备方法:中国,CN201910656913.1[P].2019-09-24.
YAN Z W,ZHENG S F,JIANG Z M,et al.Sea cucumber intestinal enzymes and its preparation methods:China,CN201910656913.1[P].2019-09-24.
[19] 崔方超,李婷婷,杨兵,等.电子鼻结合GC-MS分析草鱼脱腥前后风味变化[J].食品科学,2014,35(20):126-130.
CUI F C,LI T T,YANG B,et al.Flavor compounds of fresh and deodorized grass carps as determined by electronic nose combined with GC-MS[J].Food Science,2014,35(20):126-130.
[20] 钱攀,马旭婷,许刚,等.美国鲢鱼挥发性成分和脱腥方法研究[J].中国食品学报,2016,16(12):169-176.
QIAN P,MA X T,XU G,et al.Study on volatile components and deodorization of silver carp[J].Journal of Chinese Institute of Food Science and Technology,2016,16(12):169-176.
[21] 刘慧,秦小明,林华娟,等.牡蛎蛋白酶解液脱腥技术的研究[J].中国食品学报,2012,12(9):78-86.
LIU H,QIN X M,LIN H J,et al.Study on deodorization techniques for oyster enzymatic hydrolysate[J].Journal of Chinese Institute of Food Science and Technology,2012,12(9):78-86.
[22] 王琼,徐宝才,于海,等.电子鼻和电子舌结合模糊数学感官评价优化培根烟熏工艺[J].中国农业科学,2017,50(1):161-170.
WANG Q,XU B C,YU H,et al.Electronic nose and electronic tongue combined with fuzzy mathematics sensory evaluation to optimize bacon smoking procedure[J].Scientia Agricultura Sinica,2017,50(1):161-170.
[23] 王佳音,王凤玲,王福东,等.电子鼻对不同种类抹茶香气成分的辨别与分析[J].食品与发酵工业,2019,45(19):270-276.
WANG J Y,WANG F L,WANG F D,et al.Identification and analysis of the aroma components of different species of matcha by electronic nose[J].Food and Fermentation Industries,2019,45(19):270-276.
[24] 王晓君,王振华,王亚娜,等.泥鳅不同脱腥方法比较及腥味物质分析[J].食品科学,2016,37(15):124-129.
WANG X J,WANG Z H,WANG Y N,et al.Comparison of different deodorization methods for loach and analysis of odor substances[J].Food Science,2016,37(15):124-129.
[25] 顾赛麒,胡彬超,张月婷,等.基于电子鼻、气-质联用技术和感官评价方法优化海带发酵脱腥工艺[J].食品与发酵工业,2020,46(19):124-129.
GU S Q,HU B C,ZHANG Y T,et al.Optimization of fermentation deodorization of Laminaria japonica based on electronic nose,GC-MS and sensory evaluation[J].Food and Fermentation Industries,2020,46(19):124-129.
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