食品与发酵工业 >

2022 , Vol. 48 >Issue 8: 55 - 63

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028724

研究报告

羧甲基纤维素诱导培养下罗伦隐球酵母的转录组差异分析

  • 马电通 ,
  • 常雪苗 ,
  • 李文清 ,
  • 黄二宾 ,
  • 杜嵘宇 ,
  • 杨清 ,
  • 王芳 ,
  • 邓佳
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  • 1(西南林业大学 林学院,云南 昆明,650224)
    2(西南地区生物多样性保育国家林业局重点实验室,云南 昆明,650224)
    3(西南山地森林资源保育与利用教育部重点实验室,云南 昆明,650224)
第一作者:硕士研究生(邓佳教授为通信作者,E-mail:dengjia1983@163.com)

收稿日期: 2021-07-27

  修回日期: 2021-08-17

  网络出版日期: 2022-05-18

基金资助

国家自然科学基金(31960326;32160394)

收起

Differential analysis of the transcriptome of Cryptococcus laurentii under carboxymethyl cellulose induced culture

  • MA Diantong ,
  • CHANG Xuemiao ,
  • LI Wenqing ,
  • HUANG Erbin ,
  • DU Rongyu ,
  • YANG Qing ,
  • WANG Fang ,
  • DENG Jia
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  • 1(College of Forestry, Forestry College of Southwest, Kunming 650224, China)
    2(Key laboratory of Biodiversity Conservation in Southwest China, State Forest Administration, Kunming 650224, China)
    3(Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education, Kunming 650224, China)

Received date: 2021-07-27

  Revised date: 2021-08-17

  Online published: 2022-05-18

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摘要

为探究罗伦隐球酵母(Cryptococcus laurentii)响应羧甲基纤维素(carboxymethyl cellulose,CMC)诱导培养的分子机理,为多糖诱导C.laurentii生理代谢相关基因的挖掘提供理论依据。该实验以不加CMC培养的C.laurentii为对照组,加0.5%(质量分数) CMC培养的C.laurentii为处理组(0.5% CMC),观察了CMC诱导培养对C.laurentii细胞生长形态变化。在此基础上,通过Illumina高通量测序技术,对2组酵母菌进行转录组测序,并采用生物信息学方法对数据分析。结果表明,添加0.5%(质量分数) CMC培养后,部分酵母细胞形态呈椭圆形,0.5% CMC诱导培养24～72 h,酵母细胞形态变化率显著(P＜0.05)高于对照组。转录组数据分析表明,CMC诱导培养24 h,C.laurentii共有58个差异基因(differential genes,DEGs),其中55个为上调表达,3个为下调表达。DEGs被注释到24条GO(gene ontology)分类中和14条KEGG(Kyoto Encyclopedia of Genes and Genomes)通路中,分析结果表明,0.5% CMC诱导培养24 h C.laurentii涉及的DEGs功能主要与细胞能量代谢和生长、繁殖等有关。上述结果可以为多糖诱导培养对C.laurentii的生长影响及提高酵母拮抗效力分子机制的进一步研究提供科学依据,其可为C.laurentii形态变化相关基因挖掘提供理论基础,并为今后C.laurentii的商业化应用提供了一定的现实指导意义。

关键词： 罗伦隐球酵母; 羧甲基纤维素; 转录组; 诱导; 生物学信息分析

本文引用格式

马电通 , 常雪苗 , 李文清 , 黄二宾 , 杜嵘宇 , 杨清 , 王芳 , 邓佳 . 羧甲基纤维素诱导培养下罗伦隐球酵母的转录组差异分析[J]. 食品与发酵工业, 2022 , 48(8) : 55 -63 . DOI: 10.13995/j.cnki.11-1802/ts.028724

Abstract

To investigate the molecular mechanism of Cryptococcus laurentii in response to carboxymethyl cellulose (CMC) induction culture, and to provide a theoretical basis for the excavation of genes related to polysaccharide-induced physiological metabolism of C. laurentii, C. laurentii cultured without CMC was set as the control group and C. laurentii yeast cultured with 0.5% CMC as the treatment group (0.5% CMC), and the morphological changes of CMC-induced culture on the cell growth of C. laurentii were observed. The transcriptomes of these two yeast groups were sequenced by Illumina high-throughput sequencing, and the data were analyzed by bioinformatics methods. The results showed that some yeast cells were oval in morphology after the addition of 0.5% CMC in culture, and the rate of morphological change of yeast cells was significantly (P<0.05) higher in 0.5% CMC induced culture for 24-72 h than that of the control group. Transcriptome data analysis showed that there were 58 differential expressed genes (DEGs) in C. laurentii at 24 h of CMC induction, of which 55 were up-regulated and three were down-regulated. DEGs were annotated into 24 GO classifications and 14 KEGG pathways, and the analysis showed that the functions of DEGs in C. laurentii were mainly related to cellular energy metabolism and growth and reproduction when induced by 0.5% CMC for 24 h. The results provide a scientific basis for further research on the effect of polysaccharide-induced culture on the growth of C. laurentii and the molecular mechanism to improve the antagonistic potency of yeast, which can provide a theoretical basis for the excavation of genes related to morphological changes in C. laurentii and practical guidance for the future commercial application of C. laurentii.

Key words： Cryptococcus laurentii; carboxymethyl cellulose; transcriptome; induced; biological information analysis

参考文献

[1] 毛淑波. 罗伦隐球酵母结合热空气处理对草莓采后病害的防治及其机理研究[D].南京:南京农业大学, 2013.
MAO S B.Study on effect and mechanism of antagonistic yeast in combination with hot air treatment on postharvest disease of strawberry[D].Nanjing:Nanjing Agricultural University, 2013.
[2] 胡浩. 西藏罗伦隐球酵母对水果采后病害的防治效果及相关机理研究[D].杭州:浙江大学,2016.
HU H.Biocontrol of fruit postharvest decay by Tibetan Cryptococcus laurentii and its action mechanism[D].Hangzhou:Zhejiang University, 2016.
[3] 刘普, 方静凡, 程运江, 等.生防酵母菌防治果品采后病害机理的研究进展[J].华中农业大学学报, 2013, 32(2):134-140.
LIU P, FANG J F, CHENG Y J, et al.Research progress on action mechanism of biocontrol yeast against postharvest fruit pathogen[J].Journal of Huazhong Agricultural University, 2013, 32(2):134-140.
[4] SUI Y, WISNIEWSKI M, DROBY S, et al.Responses of yeast biocontrol agents to environmental stress[J].Applied and Environmental Microbiology, 2015, 81(9):2 968-2 975.
[5] LI B Q, TIAN S P.Effects of trehalose on stress tolerance and biocontrol efficacy of Cryptococcus laurentii[J].Journal of Applied Microbiology, 2006, 100(4):854-861.
[6] FIORI S, SCHERM B, LIU J, et al.Identification of differentially expressed genes associated with changes in the morphology of Pichia fermentans on apple and peach fruit[J].FEMS Yeast Research, 2012, 12(7):785-795.
[7] 迟孟山. 酵母拮抗菌形态转变对逆境耐受性和生防效力的影响研究[D].合肥:合肥工业大学,2017.
CHI M S.Effect of morphology change of antagonistic yeast on stress tolerance and biocontrol efficacy[D].Hefei:Hefei University of Technology, 2017.
[8] 王东升, 田晓娟, 黄江丽, 等.MIG1基因和葡萄糖对扣囊复膜孢酵母细胞形态变化的影响及机理探究[J].微生物学通报, 2014, 41(9):1 757-1 763.
WANG D S, TIAN X J, HUANG J L, et al.Effects of MIG1 gene and glucose on cell morphology of Saccharomycopsis fibuligera and mechanism exploration[J].Microbiology, 2014, 41(9):1 757-1 763.
[9] 杨金姝. 羧甲基纤维素钠在食品工业中的应用研究[J].农产品加工·学刊(下), 2014(11):76-78.
YANG J S.Application of sodium carboxymethyl cellulose in food industry[J].Academic Periodical of Farm Products Processing, 2014(11):76-78.
[10] 王芸. β-葡聚糖诱导提高Cryptococcus podzolicus对苹果青霉病的防治效力及其机制研究[D].镇江:江苏大学, 2018.
WANG Y.Exploring the effect of β-glucan on the biocontrol activity of Cryptococcus podzolicus against postharvest decay of apples and the possible mechanisms involved[D].Zhenjiang:Jiangsu University, 2018.
[11] 杨新, 陈莉, 杨双全, 等.不同培养条件下酿酒酵母菌的转录组差异分析[J].食品与发酵工业, 2021, 47(4):102-109.
YANG X, CHEN L, YANG S Q, et al.Transcriptome analysis of Saccharomyces cerevisiae under different culture conditions[J].Food and Fermentation Industries, 2021, 47(4):102-109.
[12] 王子恒, 杜宥呈, 陈璐璐, 等.肿瘤微环境对肿瘤细胞能量代谢调节的研究进展[J].交通医学, 2020, 34(2):155-158.
WANG Z H, DU Y C, CHEN L L, et al.Research progress on the regulation of energy metabolism in tumor cells by tumor microenvironment[J].Medical Journal of Communications, 2020, 34(2):155-158.
[13] 文禹粱, 刘秀, 王继卿, 等.哺乳动物线粒体动力学和氧化磷酸化研究进展[J].畜牧兽医学报, 2021, 52(2):273-285.
WEN Y L, LIU X, WANG J Q, et al.Research progress of mitochondrial dynamics and oxidative phosphorylation in mammal[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2):273-285.
[14] CHABAN Y, BOEKEMA E J, DUDKINA N V.Structures of mitochondrial oxidative phosphorylation super complexes and mechanisms for their stabilisation[J].Biochimica et Biophysica Acta, 2014, 1 837(4):418-426.
[15] 曾琪琦, 蒋湘宁, 盖颖.毛白杨细胞色素C还原酶的基因克隆、表达和亚细胞定位[J].分子植物育种, 2020, 18(8):2 423-2 430.
ZENG Q Q, JIANG X N, GAI Y.Gene cloning, expression and subcellular location of cytochrome C reductase from Populus tomentosa[J].Molecular Plant Breeding, 2020, 18(8):2 423-2 430.
[16] 龙红, 牛熙, 黄世会, 等.沙福芽孢杆菌氧化磷酸化通路相关基因对锰胁迫的应答[J].工业微生物, 2020, 50(4):27-33.
LONG H, NIU X, HUANG S H, et al.Response of genes related to oxidative phosphorylation pathway of Bacillus safensis under manganese stress[J].Industrial Microbiology, 2020, 50(4):27-33.
[17] 李佳萍, 余功, 谢斌.清燥救肺汤对Lewis肺癌荷瘤小鼠肺癌组织氧化磷酸化能量代谢的影响[J].中医杂志, 2021, 62(5):439-444.
LI J P, YU G, XIE B.Effect of Qingzao Jiufei decoctionon energy metabolism of oxidative phosphorylation in lung carcinoma tissue of Lewis tumor-bearing mice[J].Journal of Traditional Chinese Medicine, 2021, 62(5):439-444.
[18] WALKER J E.The ATP synthase:The understood, the uncertain and the unknown[J].Biochemical Society Transactions, 2013, 41(1):1-16.
[19] 宋志强, 丁祥, 唐贤, 等.松乳菇子实体两个发育时期的转录组分析[J].浙江农业学报, 2020, 32(2):337-347.
SONG Z Q, DING X, TANG X, et al.Transcriptome analysis of fruiting bodies of Lactarius deliciosus at two developmental stages[J].Acta Agriculturae Zhejiangensis, 2020, 32(2):337-347.
[20] 闫岩, 王明力, 李岑, 等.几丁质诱导汉逊德巴利酵母拮抗活性的研究[J].现代食品科技, 2014, 30(1):91-95.
YAN Y, WANG M L, LI C, et al.Antagonistic activity of Debaryomyceshansenii induced by chitin[J].Modern Food Science and Technology, 2014, 30(1):91-95.
[21] 韩秀娟, 刘清, 郑树涛, 等.CALM1在食管鳞癌细胞及组织水平中的表达[J].新疆医科大学学报, 2020, 43(4):439-443;448.
HAN X J, LIU Q, ZHENG S T, et al.Expression of CALM1 in esophageal squamous cell carcinoma cells and tissues[J].Journal of Xinjiang Medical University, 2020, 43(4):439-443;448.
[22] 胡亮, 郭东伟, 李江南, 等.猪繁殖与呼吸综合征病毒Nsp9与宿主CALM2基因编码钙调蛋白的相互作用研究[J].中国预防兽医学报, 2015, 37(9):674-678.
HU L, GUO D W, LI J N, et al.Identification of the host CALM2 gene-encoded calmodulin interacting with porcine reproductive and respiratory syndrome virus Nsp9[J].Chinese Journal of Preventive Veterinary Medicine, 2015, 37(9):674-678.
[23] 周凤, 郑韦韦, 刘鹏飞, 等.钙调蛋白质与富勒烯结合模式的分子对接研究[J].湖北医药学院学报, 2019, 38(5):422-425;414;515.
ZHOU F, ZHENG W W, LIU P F, et al.Molecular docking study on the binding mode of calmodulin and fullerene[J].Journal of Hubei University of Medicine, 2019, 38(5):422-425;414;515.
[24] KURSULA P.The many structural faces of calmodulin:A multitasking molecular jackknife[J].Amino Acids, 2014, 46(10):2 295-2 304.
[25] 樊纯. 酿酒酵母AFR1过量表达与MPK1及MIH1缺失导致的合成致死[D].天津:天津大学, 2009.
FAN C.Synthetic lethality resulted from AFR1 over-expression and MIH1/MPK1 deletion in Saccharomyces cerevisiae[D].Tianjin:Tianjin University, 2009.
[26] PAL G, PARAZ M T Z, KELLOGG D R.Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1[J].The Journal of Cell Biology, 2008, 180(5):931-945.
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