食品与发酵工业 >

2022 , Vol. 48 >Issue 8: 90 - 97

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028601

研究报告

植物乳杆菌发酵豆乳对多囊卵巢综合征大鼠肠道菌群紊乱的改善作用

  • 祁亚劲 ,
  • 贺禹丰 ,
  • 杨羽倩 ,
  • 石刘婷 ,
  • 詹语 ,
  • 鲁佳欣 ,
  • 郭敏 ,
  • 王慧玲 ,
  • 赵建新 ,
  • 张灏 ,
  • 王刚 ,
  • 陈卫
展开
  • 1(江南大学 食品学院,江苏 无锡,214122)
    2(苏州市立医院, 南京医科大学附属苏州医院,南京医科大学 姑苏学院,江苏 苏州,215002)
第一作者:本科生(王慧玲主任医师和王刚教授为共同通信作者,E-mail:13806132001@139.com;wanggang@jiangnan.edu.cn)

收稿日期: 2021-07-14

  修回日期: 2021-08-18

  网络出版日期: 2022-05-18

基金资助

省级大学生创新创业训练计划项目(202010295134Y);苏州市科技计划-关键技术应用研究(SS201873)

收起

Alleviation effects of soymilk fermented by Lactobacillus plantarum on gut microbiota dysbiosis in rats with polycystic ovary syndrome

  • QI Yajin ,
  • HE Yufeng ,
  • YANG Yuqian ,
  • SHI Liuting ,
  • ZHAN Yu ,
  • LU Jiaxin ,
  • GUO Min ,
  • WANG Huiling ,
  • ZHAO Jianxin ,
  • ZHANG Hao ,
  • WANG Gang ,
  • CHEN Wei
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  • 1(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    2(Suzhou Municipal Hospital, Suzhou Hospital Affiliated to Nanjing Medical University, Gusu College of Nanjing Medical University, Suzhou 215002, China)

Received date: 2021-07-14

  Revised date: 2021-08-18

  Online published: 2022-05-18

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摘要

前期研究发现若干植物乳杆菌CCFM1019、LP4、LP5的发酵豆乳可以改善多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠模型的部分病理指标。为进一步研究病理指标改善与肠道内环境变化之间的关系,对实验大鼠肠道菌群进行了分析并测定了粪便短链脂肪酸水平。此外,对模型大鼠肠道菌群丰度、短链脂肪酸变化及性激素水平进行相关性分析。结果表明,植物乳杆菌CCFM1019、LP4、LP5发酵豆乳显著提高了PCOS大鼠肠道菌群的多样性。与模型组相比,3株植物乳杆菌发酵豆乳恢复了Lactobacillus、Prevotellaceae UCG-001的正常相对丰度。相关性分析结果显示,Lactobacillus的相对丰度与睾酮(testosterone,T)水平呈正相关;Lachnospira的相对丰度与雌二醇(estradiol,E2)水平呈正相关,与促黄体生成素(luteinizing hormone,LH)水平呈负相关;Prevotellaceae UCG-001、Ruminococcaceae UCG-014的相对丰度与促性腺激素释放激素(gonadotropin-releasing hormone,GnRH)水平呈正相关。然而,植物乳杆菌发酵豆乳对短链脂肪酸水平的影响并不显著。综上,植物乳杆菌CCFM1019、LP4、LP5发酵豆乳对PCOS大鼠肠道菌群的多样性及组成影响显著,同时这些影响与性激素水平存在显著相关性。这些结果提示,调节肠道菌群可能是植物乳杆菌发酵豆乳改善PCOS大鼠激素水平紊乱的潜在作用机制。

关键词： 植物乳杆菌; 发酵豆乳; 多囊卵巢综合征; 肠道菌群; 性激素

本文引用格式

祁亚劲 , 贺禹丰 , 杨羽倩 , 石刘婷 , 詹语 , 鲁佳欣 , 郭敏 , 王慧玲 , 赵建新 , 张灏 , 王刚 , 陈卫 . 植物乳杆菌发酵豆乳对多囊卵巢综合征大鼠肠道菌群紊乱的改善作用[J]. 食品与发酵工业, 2022 , 48(8) : 90 -97 . DOI: 10.13995/j.cnki.11-1802/ts.028601

Abstract

It has been found that soymilk fermented by Lactobacillus plantarum CCFM1019, LP4 or LP5 can improve some pathological indicators of polycystic ovary syndrome (PCOS) rat model in previous study. In order to further explore the relationship between the improvement of pathological indicators and the changes in the intestinal environment, the gut microbiota and the level of fecal short-chain fatty acids (SCFAs) were analyzed. In addition, the correlation of the gut microbiota, SCFAs and sex hormone levels in rats were analyzed. The results showed that soymilk fermented by L. plantarum CCFM1019, LP4 or LP5 significantly increased the diversity of gut microbiota in PCOS rats. Compared with the letrozole group, soymilk fermented by three strains of L. plantarum significantly restored the relative abundance of Lactobacillus and Prevotellaceae UCG-001 to normal levels. It was indicated that the relative abundance of Lactobacillus was positively correlated with the level of testosterone (T). The relative abundance of Lachnospira was positively correlated with the estradiol level and negatively correlated with the luteinizing hormone level. The relative abundance of Prevotellaceae UCG-001 and Ruminococcaceae UCG-014 were positively correlated with the gonadotropin-releasing hormone level. However, the effects of L. plantarum fermented soymilk on the level of SCFAs were not significant. In conclusion, soymilk fermented by L. plantarum CCFM1019, LP4 or LP5 significantly affected the diversity and composition of gut microbiota in PCOS rats. And these effects were significantly correlated with sex hormone levels. These results suggest that regulating the gut microbiota may be the potential mechanism of L. plantarum fermented soymilk to alleviate the disorder of hormone levels in PCOS rats.

Key words： Lactobacillus plantarum; fermented soymilk; polycystic ovary syndrome; gut microbiota; sex hormones

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