食品与发酵工业 >

2022 , Vol. 48 >Issue 8: 270 - 275

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028128

分析与检测

川产竹叶花椒挥发油中12种挥发性成分的测定与比较

  • 杨森 ,
  • 何晗 ,
  • 易达 ,
  • 王福 ,
  • 刘友平 ,
  • 陈林 ,
  • 陈鸿平
展开
  • 1(成都中医药大学 药学院,四川 成都,611137)
    2(四川省南充市食品药品检验所,四川 南充,637002)
第一作者:硕士研究生(陈林副教授和陈鸿平教授为共同通信作者,E-mail:chenlin@cdutcm.edu.cn;chen_hongping@126.com)

收稿日期: 2021-05-27

  修回日期: 2021-07-20

  网络出版日期: 2022-05-18

基金资助

四川省科技计划项目(2019YFS0159);成都中医药大学西南特色中药资源重点实验室开放研究基金资助项目(2020QNJS002)

收起

Determination and comparison of 12 volatile components in volatile oil from Zanthoxylum armatum DC.

  • YANG Sen ,
  • HE Han ,
  • YI Da ,
  • WANG Fu ,
  • LIU Youping ,
  • CHEN Lin ,
  • CHEN Hongping
Expand
  • 1(School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China)
    2(Nanchong City, Sichuan Province Food and Drug Inspection and Testing Institute, Nanchong 637002, China)

Received date: 2021-05-27

  Revised date: 2021-07-20

  Online published: 2022-05-18

Fold

摘要

为建立同时测定竹叶花椒挥发油中α-蒎烯、桧烯、β-蒎烯、月桂烯、D-柠檬烯、γ-松油烯、萜品油烯、芳樟醇、α-松油醇、乙酸芳樟酯、α-石竹烯、β-石竹烯12种成分含量的方法,采用水蒸气蒸馏法对不同产地的竹叶花椒中挥发油进行提取,以1-壬醇作为内标,采用气相色谱-质谱/选择离子监测模式法建立了竹叶花椒挥发油中的12种挥发性成分测定的方法,并对不同产地竹叶花椒挥发油中的挥发性成分进行测定。所建立的方法,能够使样品中的各指标成分达到良好的分离,在考察范围内,竹叶花椒挥发油中12种挥发性成分与内标物的峰面积比值与其质量浓度具有良好的线性关系(r>0.999 0),样品加标回收率为98.33%~107.09%,相对标准偏差均小于6%。所测定样品中均含有上述12种挥发性成分,且整体上芳樟醇(293.938~413.975 mg/mL)、桧烯(33.858~123.442 mg/mL)、D-柠檬烯(28.832~51.788 mg/mL)、月桂烯(13.570~33.445 mg/mL)、乙酸芳樟酯(2.540~24.212 mg/mL)、α-石竹烯(9.115~15.085 mg/mL)、β-石竹烯(6.111~16.675 mg/mL)含量较高。该方法简便、迅速,结果准确,可用于竹叶花椒挥发油的质量控制。

关键词： 竹叶花椒; 挥发油; 气相质谱-质谱联用; 挥发性成分; 定量方法

本文引用格式

杨森 , 何晗 , 易达 , 王福 , 刘友平 , 陈林 , 陈鸿平 . 川产竹叶花椒挥发油中12种挥发性成分的测定与比较[J]. 食品与发酵工业, 2022 , 48(8) : 270 -275 . DOI: 10.13995/j.cnki.11-1802/ts.028128

Abstract

The aim of this study was to establish a method for simultaneously determine the content of 12 components of α-pinene, Sabinene, β-pinene, myrcene, D-limonene, γ-terpinene, terpinolene, linalool, α-terpineol, linalyl acetate, α-caryophyllene, β-caryophyllene in the volatile oil of Zanthoxylum armatum DC.. Steam distillation was used to extract volatile oil from Zanthoxylum armatum DC. from different origins. Determine the content of 12 volatile components in the volatile oil of Zanthoxylum armatum DC. With 1-nonanol as the internal standard, gas chromatography-mass spectrometry/selected ion monitor technique was used to determine the content of 12 volatile components in the volatile oil of Zanthoxylum armatum DC.. The results showed that the established method could achieve a good separation of the various index components in the sample. In the scope of the investigation, the peak area ratio of the 12 volatile components in the volatile oil of Zanthoxylum armatum DC. and the internal standard substance had good linearity with the mass concentration. The relationship (r>0.999 0), the recovery rate of sample addition was 98.33%-107.09%, and the relative standard deviation was less than 6%. The above-mentioned 12 volatile components were found to be presented in all measured samples, and linalool (293.938-413.975 mg/mL), sabinene (33.858-123.442 mg/mL), D-limonene (28.832-51.788 mg/mL), myrcene (13.570-33.445 mg/mL), linalyl acetate (2.540-24.212 mg/mL), α-caryophyllene (9.115-15.085 mg/mL), β-caryophyllene (6.111-16.675 mg/mL) had higher contents. The method developed here is simple, rapid and accurate, and can be used for the quality control of the volatile oil of Zanthoxylum armatum DC..

Key words： Zanthoxylum armatum DC.; volatile oil; gas chromatoraphy-mass spectrometry; volatile components; quantitative me

参考文献

[1] 祝磊, 陈茜, 黎江华, 等.藤椒来源的考证与定义的思考[J].中草药, 2018, 49(4):987-992.
ZHU L, CHEN X, LI J H, et al.Textual research on origin of Zanthoxylum armatum and consideration of its definition[J].Chinese Traditional and Herbal Drugs, 2018, 49(4):987-992.
[2] 樊丹青, 刘友平, 陈鸿平.花椒本草考证[J].中药与临床, 2013, 4(6):59-61.
FAN D Q, LIU Y P, CHEN H P.Bencaological study of Zanthoxylum schinifolium Sieb.et Zucc.and Zanthoxylum bungeanum Maxim.[J].Pharmacy and Clinics of Chinese Materia Medica, 2013, 4(6):59-61.
[3] 姚佳, 蒲彪.青花椒的研究进展[J].中国调味品, 2010, 35(6):35-39.
YAO J, PU B.Research progress on Zanthoxylum schinifolium Sieb.et Zucc.[J].China Condiment, 2010, 35(6):35-39.
[4] 陈茜, 陶兴宝, 黄永亮, 等.花椒香气研究进展[J].中国调味品, 2018, 43(1):189-194.
CHEN Q, TAO X B, HUANG Y L, et al.Research progress on the aroma of Chinese prickly ash[J].China Condiment, 2018, 43(1):189-194.
[5] 杨玉贤, 高云涛, 邹维, 等.双溶剂微池顶空萃取气相色谱-质谱联用分析竹叶花椒中挥发性成分[J].食品与发酵工业, 2020, 46(22):214-219.
YANG Y X, GAO Y T, ZOU W, et al.GC-MS analysis of volatile components in Zanthoxylum armatum by double solvent micro cell headspace extraction[J].Food and Fermentation Industries, 2020, 46(22):214-219.
[6] 罗凯, 朱琳, 阚建全.水蒸汽蒸馏、溶剂萃取、同时蒸馏萃取法提取花椒挥发油的效果比较[J].食品科技, 2012,37(10):234-236;240.
LUO K, ZHU L, KAN J Q.Comparison on extraction of steam distillation, solvent, simultaneous distillation for Zanthoxylum essential oil[J].Food Science and Technology, 2012,37(10):234-236;240.
[7] 王世敏, 王嘉林, 程金朋, 等.GC-MS分析比较昭通产红花椒与青花椒的挥发油组分[J].海峡药学, 2018, 30(1):45-47.
WANG S M, WANG J L, CHENG J P, et al.Evaluation on the essential oils quality from Zhaotong Zanthoxylum bungeanum Maxim.and Zanthoxylum schinigolium Sieb.et Zucc by GC-MS[J].Strait Pharmaceutical Journal, 2018, 30(1):45-47.
[8] 边甜甜, 司昕蕾, 牛江涛, 等.花椒及不同炮制品挥发油与总生物碱的比较研究[J].时珍国医国药, 2018, 29(12):2 937-2 939.
BIAN T T, SI X L, NIU J T, et al.Comparative study on volatile oils and total alkaloids from Zanthoxylum bungeanum and different processed products[J].Lishizhen Medicine and Materia Medica Research, 2018, 29(12):2 937-2 939.
[9] 徐双美, 杨森, 李泽娜, 等.GC-MS分析四川主产地竹叶花椒的挥发性成分[J].中国调味品, 2020, 45(12):124-129;133.
XU S M, YANG S, LI Z N, et al.Analysis of volatile components from Zanthoxylum armatum DC.in Sichuan province by GC-MS[J].China Condiment, 2020, 45(12):124-129;133.
[10] 刘飞, 梅国荣, 卢俊宇, 等.GC-MS-AMDIS结合保留指数分析比较花椒与青椒挥发油的组成[J].中国实验方剂学杂志, 2017, 23(5):84-89.
LIU F, MEI G R, LU J Y, et al.Analysis on essential oil from Zanthoxylum bungeanum and Zanthoxylum schinifolium by GC-MS-AMDIS combined with kováts retention index[J].Chinese Journal of Experimental Traditional Medical Formulae, 2017, 23(5):84-89.
[11] 杨潇, 芮光伟, 蒋珍菊.三种不同方法提取花椒挥发油中化学成分的GCMS/AMDIS比较分析[J].中国调味品, 2014,39(5):118-121;133.
YANG X, RUI G W, JIANG Z J.Comparative analysis of GCMS/AMDIS for chemical components in volatile oils from Zanthoxylum bungeanum Maxim.with three different extraction methods[J].China Condiment, 2014,39(5):118-121;133.
[12] 哈立洋, 杨斌, 尹可欢, 等.竹叶花椒挥发油提取工艺优化及镇痛抗炎活性研究[J].中药药理与临床, 2021, 37(3):127-132.
HA L Y, YANG B, YIN K H, et al.Extraction process optimization of volatile oil from Zanthoxylum armatum DC.and study of analgesic and anti-inflammatory activity[J].Pharmacology and Clinics of Chinese Materia Medica, 2021, 37(3):127-132.
[13] 干信, 吴士筠, 高雯琪.花椒挥发油抑菌作用研究[J].食品科学, 2009, 30(21):128-130.
GAN X, WU S J, GAO W Q.Antimicrobial activity of volatile oil from peppers[J].Food Science, 2009, 30(21):128-130.
[14] 蒋丽艳, 刘继鑫, 张浩, 等.花椒挥发油对两种人体蠕形螨的体外抑杀作用[J].医学研究杂志, 2009, 38(2):78-79.
JIANG L Y, LIU J X, ZHANG H, et al.The effect of the volatile oil of Zanthoxylum bungeanum on human Demodex in vitro[J].Journal of Medical Research, 2009, 38(2):78-79.
[15] 邓凯文, 李静, 侯君, 等.花椒挥发油提取并测定其柠檬烯含量[J].西南国防医药, 2020, 30(9):793-796.
DENG K W, LI J, HOU J, et al.Extraction of essential oil from Zanthoxylum bungeanum Maxim.and determination of limonene content[J].Medical Journal of National Defending Forces in Southwest China, 2020, 30(9):793-796.
[16] 司昕蕾, 蔡秀荣, 曹瑞, 等.基于GC-MS技术对甘肃陇南大红袍花椒挥发油柠檬烯 芳樟醇含量的测定[J].中国现代中药, 2019, 21(2):173-175;193.
SI X L, CAI X R, CAO R, et al.Determination of limonene and linalool in volatile oil of Dahongpao Zanthoxylum bungeanum in Longnan by GC-MS[J].Modern Chinese Medicine, 2019, 21(2):173-175;193.
[17] 卢俊宇, 梅国荣, 刘飞, 等.GC-MS-AMDIS结合保留指数分析比较青椒与竹叶花椒挥发油的组成成分[J].中药与临床, 2015, 6(5):18-21;35.
LU J Y, MEI G R, LIU F, et al.The analysis and comparison of components of volatile oil in Zanthoxylum schinifolium sieb.et Zucc.and Zanthoxylum armatum DC.by combination of GC-MS-AMDIS and retention index[J].Pharmacy and Clinics of Chinese Materia Medica, 2015, 6(5):18-21;35.
[18] TIWARY M, NAIK S N, TEWARY D K, et al.Chemical composition and larvicidal activities of the essential oil of Zanthoxylum armatum DC.(Rutaceae) against three mosquito vectors[J].Journal of Vector Borne Diseases, 2007, 44(3):198-204.
[19] 国家药典委员会. 中华人民共和国药典(四部)[M].北京:中国医药科技出版社, 2020.
Chinese Pharmacopoeia Commission.Pharmacopoeia of the People′s Republic of China (IV)[M].Beijing:China Medical Science and Technology Press, 2020.
[20] 杨森, 冯靖雯, 刘友平, 等.热风干燥温度对竹叶花椒干燥特性及品质的影响[J].食品与发酵工业, 2021, 47(12):203-209.
YANG S, FENG J W, LIU Y P, et al.The effect of hot air drying temperature on the drying characteristics and quality of Zanthoxylum armatum DC.[J].Food and Fermentation Industries, 2021, 47(12):203-209.
[21] 李越峰, 张淑娟, 边甜甜, 等.花椒不同炮制品中挥发性成分GC-MS分析[J].天然产物研究与开发, 2020, 32(9):1 470-1 476.
LI Y F, ZHANG S J, BIAN T T, et al.GC-MS analysis of volatile components in different processed Zanthoxylum bungeanum[J].Natural Product Research and Development, 2020, 32(9):1 470-1 476.
[22] 吴素玲, 张锋伦, 孙晓明, 等.杀青等处理对云南昭通青花椒挥发性成分变化的影响[J].中国野生植物资源, 2016, 35(2):19-21;53.
WU S L, ZHANG F L, SUN X M, et al.Effects of fixation treatment on the changes of essential oil components of Zanthoxylum armatum var.novemfolius in Zhaotong, Yunnan[J].Chinese Wild Plant Resources, 2016, 35(2):19-21;53.
[23] TAN S Y, NIU Y J, LIU L, et al.Development of a GC-MS/SIM method for the determination of phytosteryl esters[J].Food Chemistry, 2019, 281:236-241.
[24] 任恒鑫, 吕玉光, 宗希明, 等.GC-MS/SIM法同时测定臭冷杉挥发油中柠檬烯和醋酸龙脑酯含量[J].中国野生植物资源, 2018, 37(1):41-43;50.
REN H X, LYU Y G, ZONG X M, et al.Simultaneous determation of limonene and bornyl acetate in the essential oil from Abies nephrolepis by GC-MS/SIM[J].Chinese Wild Plant Resources, 2018, 37(1):41-43;50.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司